EMAGE mouse gene expression spatial database

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EMAGE:31425

Plac8 ( MGI:2445289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005548_01	euxassay_005548_02	euxassay_005548_03	euxassay_005548_04	euxassay_005548_05

euxassay_005548_06	euxassay_005548_07	euxassay_005548_08	euxassay_005548_09	euxassay_005548_10

euxassay_005548_11	euxassay_005548_12	euxassay_005548_13	euxassay_005548_14	euxassay_005548_15

euxassay_005548_16	euxassay_005548_17	euxassay_005548_18	euxassay_005548_19	euxassay_005548_20

euxassay_005548_21	euxassay_005548_22	euxassay_005548_23	euxassay_005548_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 12 13 14

hindgut

strong

regional

strong expression: see section 11 12 13 14 15 16

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

liver right lobe

strong

single cell

strong expression: see section 12 13 14 15 16 17 18 19 20 21 22 23 24

stomach

strong

regional

strong expression: see section 09 10 moderate expression: see section 01 02 03 04 05 06 07 08

kidney calyx

moderate

regional

moderate expression: see section 05 06 07 08 09 15 16 17 18 19 weak expression: see section 14

rectum

strong

regional

strong expression: see section 13

midgut

strong

regional

strong expression: see section 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 08

oculomotor iii nerve

strong

regional

strong expression: see section 11 12 13 14 15 moderate expression: see section 10 16 weak expression: see section 09

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2720
Entity Detected:	Plac8, ( MGI:2445289)
Sequence:	sense strand is shown >T2720 TCNAGCCAGATTCGGCACGAGGGGTCTTTTGAGACCTCGCATCGAAGATGGCTCAGGCACCAACAGTTAT CGTGACTCAACCTGGATTCGTTCGTGCTCCCCAAAATTCCAACTGGCAGACCAGCCTGTGTGATTGCTTC AGTGACTGCGGAGTCTGCCTCTGTGGGACCTTTTGTTTCACTTGTCTTGGATGTCAAGTGGCAGCTGACA TGAATGAGTGTTGTCTGTGTGGAACAACGGTGGCCATGAGGACTCTCTACCGAACCCGATACGGCATTCC TGGATCTATTTGTGATGACTACATGGTCACACTCTTCTGTCCTGTTTGCTCTGTGTGCCAACTCAAGAGA GACATTAACAGGAGGAGAGCCATGAACGCTTTCTAAGGAGCTGGATGGCAAGAGCTCTGGCTGAAGAAGC TCAACTCAGCACACACTCCTTCAGCCTGAGATTTTTCAAATCTTTGGCAACTGAGATGGGATGGATCCAT TTAATTAGAGAACGGTGAAATCTTTCTAGTTGGGCTTTTTGATTTATTTTAAATGGATA
Notes:	The probe template was PCR amplified from IMAGE:1512918 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1512918 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005548 same experiment
	EMAGE:29570 same embryo
	EMAGE:31510 same embryo
	EMAGE:29740 same embryo
	EMAGE:31525 same embryo
	EMAGE:29569 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source