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EMAGE:31525

Slc28a2 ( MGI:1913105)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31525 EMAGE:31525 EMAGE:31525 EMAGE:31525 EMAGE:31525
euxassay_001709_01 euxassay_001709_02 euxassay_001709_03 euxassay_001709_04 euxassay_001709_05
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euxassay_001709_06 euxassay_001709_07 euxassay_001709_08 euxassay_001709_09 euxassay_001709_10
EMAGE:31525 EMAGE:31525 EMAGE:31525 EMAGE:31525 EMAGE:31525
euxassay_001709_11 euxassay_001709_12 euxassay_001709_13 euxassay_001709_14 euxassay_001709_15
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euxassay_001709_16 euxassay_001709_17 euxassay_001709_18 euxassay_001709_19 euxassay_001709_20
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euxassay_001709_21 euxassay_001709_22 euxassay_001709_23 euxassay_001709_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
foregut-midgut junction
strong strong
regionalstrong expression: see section 13 14 15
midgut
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 17 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2721
Entity Detected:Slc28a2, ( MGI:1913105)
Sequence:sense strand is shown

>T2721
GGCCTCGAGCCAGATTCGGCACGAGGGGAAGAGTGACTTGTGCAAGATTGTGGTCAGGGCCCTCTTCACA
GGTGCCTGTGTTTCCTTTATCAGTGCCTGCATGGCAGGAATCCTCTATGTTCCCAGAGGCGCTGAAACTG
ACTGTGTCTCATTCCTAAACACAAATTTCACCAATAGAACCTATGAGACATATGTGTGCTGCAGAGAGCT
TTTCCAGAGTACTTCGCTGAATGGCACCAACATGCCTTCTTTCTCAGGCCCCTGGCAAGACAATGTGTCC
AGCCTCAGGAATCTTGCTAGCTGCTGTGACCTCTATACCAGCACTGTGTGTGCATGAGGCTGGTGGGCCG
CTCCAAGACAGTTCTCATAGAACAGATTCATATTACCAAATACATTTTCATGTACTTCCTATTCTCTGAG
AACCCCACTAAACTCCACAAAGTGAAATTTCTGAGCTCACTATAGCTCTGTATAAAAATCAGCCCTTACC
TTTCCAGCGGCATGACATGAAATTCCGGTTACCAGACATGAAATTCATTATAGACCCTCTTGAGCCAAAG
TATGGTGG
Notes:The probe template was PCR amplified from IMAGE:1512943 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1512943 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001709 same experiment
 EMAGE:29570 same embryo
 EMAGE:31510 same embryo
 EMAGE:29740 same embryo
 EMAGE:31425 same embryo
 EMAGE:29569 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS