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EMAGE:31551

Ccdc80 ( MGI:1915146)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31551 EMAGE:31551 EMAGE:31551 EMAGE:31551 EMAGE:31551
euxassay_006368_01 euxassay_006368_02 euxassay_006368_03 euxassay_006368_04 euxassay_006368_05
EMAGE:31551 EMAGE:31551 EMAGE:31551 EMAGE:31551 EMAGE:31551
euxassay_006368_06 euxassay_006368_07 euxassay_006368_08 euxassay_006368_09 euxassay_006368_10
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euxassay_006368_11 euxassay_006368_12 euxassay_006368_13 euxassay_006368_14 euxassay_006368_15
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euxassay_006368_16 euxassay_006368_17 euxassay_006368_18 euxassay_006368_19 euxassay_006368_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lens
strong strong
regionalstrong expression: see section 01
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 weak expression: see section 01 07 08 09 18 19 20
extrinsic ocular muscle
strong strong
regionalstrong expression: see section 04 05 19 20
mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 07 weak expression: see section 06 12
male reproductive system
strong strong
regionalstrong expression: see section 08 12
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 14 15 16 17 weak expression: see section 13 18
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 10 12
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 08 09 10 11
bladder
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 12
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35062
Entity Detected:Ccdc80, ( MGI:1915146)
Sequence:sense strand is shown

>T35062
CGTCTCAGAGCAGCTATACCCTCTACCTCGGAAGGAGCAACAGAGAGAGAAGCCACAGGCCACCAGGAGG
CCCAGCAAAGCCACCAACTATGGAAGCTTCACAGCCACCCCACCTCCCACCCTCTGGGAGGTCAGCGCAA
GAGTTGTGGGCACAAGCCGTTTCCGGGACAACCGGACAGACAAACGGGAACATGGCCATCAGGACCCAAA
TGCGGTGCCAGGTCCTCACAAGCCAGTAAAGGGGAAGCTGCCCAAAAAGAAGGACAGAATTCTCAGCAAC
GAATATGAAGACAAGTATGACCTCAGCCAGCCCACCTCCTCTCAGGGGGAGGAGGAGCGGCGGGTGGATA
GCGTTCCCTCGCAGAATGCCAAGGAGTCGAAAAAGCTTGAAAAGCTCGAGAAACCTGAGAAGGAGAAGAA
GAAAAAAGGGAAGAGTGCAAAACAAGACAAGTTACTCAAGAGTGAAAAGCAAGCGAAGAAAGCTGAGAAA
AAGACCAAACAGGAGAAAGACAAGAATAAGAAGAAAAAGGCGGGTAAGACAGAGCAGGATGACAATCAGA
AGCCCACCGCAAAACATCTCGCTCCAAGTCCCAAGAAGTCAGTGGCCGACCTGTTGGGGTCTTTCGAAGG
CAAACGAAGACTCCTCCTGATCACCACTCCCAAGGCTGAGAACAATATGTATGTGCAGCAGCGGGATGAA
TATCTGGAGAGCTTCTGCAAGATGGCCACCAGGAGGATCTCTGTGGTCACCATCTTTGGTCCTGTCAACA
ACAGCTCCATGAAAATTGATCACTTCCAGCTAGATAATGAGAAACCCATGCGTGTGGTGGATGATGATGA
CTTGGTGGACCAACATCTCATCAGCGAGCTGAGGAAGGAGTATGGAATGACCTACGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91281. Forward Primer - name:091281_F_cDNA_2610001E17Rik, sequence:CGTCTCAGAGCAGCTATACCCT; Reverse Primer - name:091281_N_SP6_cDNA_2610001E17Rik, sequence:TCGTAGGTCATTCCATACTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006368 same experiment
 EMAGE:30713 same embryo
 EMAGE:31549 same embryo
 EMAGE:30449 same embryo
 EMAGE:30465 same embryo
 EMAGE:32119 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS