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EMAGE:31559

Crispld2 ( MGI:1926142)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31559 EMAGE:31559 EMAGE:31559 EMAGE:31559 EMAGE:31559
euxassay_006369_01 euxassay_006369_02 euxassay_006369_03 euxassay_006369_04 euxassay_006369_05
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euxassay_006369_06 euxassay_006369_07 euxassay_006369_08 euxassay_006369_09 euxassay_006369_10
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euxassay_006369_11 euxassay_006369_12 euxassay_006369_13 euxassay_006369_14 euxassay_006369_15
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euxassay_006369_21 euxassay_006369_22 euxassay_006369_23 euxassay_006369_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
esophagus
moderate moderate
regionalmoderate expression: see section 11 12
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 15 16 17
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 weak expression: see section 01
rectum
moderate moderate
regionalmoderate expression: see section 13
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
bladder
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35015
Entity Detected:Crispld2, ( MGI:1926142)
Sequence:sense strand is shown

>T35015
TGCTATCCACTACGGTGTCATCGATGATCGAGGTGGCCTGGTGGATGTCACCAGGAATGGGATGGTACCC
TTCTTTGTCAAGTCTCAGAAAAATGGCATGGAGTCCCTGAGCAAATACAAGCCGTCTAGCTCCTTCACTG
TGTCAAAAGTGACAGAGACGGCCGTGGACTGCCACGCCACGGTCGCACAGCTGTGCCCCTTCGAGAAGCC
GGCCACCCACTGCCCGAGAATCCAGTGTCCTGCGCGCTGTGGAGAAGAGCCATCCTATTGGGCTCCTGTG
TATGGAACCAACATCTATGCTGATACTTCCAGCATTTGTAAGGCCGCTGTGCACGCAGGTGTCATCGTTG
ACGAGGTTGGTGGCTATGCAGATGTGATGCCCGTGGACAAAAAGAAGAGCTACGTGGGCTCCCTCAGGAA
CGGGGTGCAGTCGGAGAGCCTGAACACTCCTCAGAACGGAAACGCCTTCCGGATCTTCGCCGTCAGGCAG
TGAATTTGGCGACCTGGGGAGAAGGGGGTGTCATCTACTGGGATTCGGGGTCCTGCTTTTTATCTGTACT
TTGTCCTTTGGGGGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 66235. Forward Primer - name:066235_F_cDNA_1810049K24Rik, sequence:TGCTATCCACTACGGTGTCATC; Reverse Primer - name:066235_N_SP6_cDNA_1810049K24Rik, sequence:ACCCCCAAAGGACAAAGTAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006369 same experiment
 EMAGE:32111 same embryo
 EMAGE:31586 same embryo
 EMAGE:30711 same embryo
 EMAGE:31621 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS