EMAGE mouse gene expression spatial database

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EMAGE:31673

Slc25a31 ( MGI:1920583)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008163_01	euxassay_008163_02	euxassay_008163_03	euxassay_008163_04	euxassay_008163_05

euxassay_008163_06	euxassay_008163_07	euxassay_008163_08	euxassay_008163_09	euxassay_008163_10

euxassay_008163_11	euxassay_008163_12	euxassay_008163_13	euxassay_008163_14	euxassay_008163_15

euxassay_008163_16	euxassay_008163_17	euxassay_008163_18	euxassay_008163_19	euxassay_008163_20

euxassay_008163_21	euxassay_008163_22	euxassay_008163_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

spotted

moderate expression: see section 09 10 11 12 13 15 16 17 18 19 20 21 weak expression: see section 01 02 03 04 22

right lung

moderate

spotted

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22

pituitary gland

moderate

spotted

moderate expression: see section 13 14 15 17 weak expression: see section 12 16

urethra of male

weak

spotted

weak expression: see section 10 11 12

testis

moderate

regional

moderate expression: see section 04 05 06 07 19 20 weak expression: see section 21

left lung

moderate

spotted

moderate expression: see section 05 06 07 09 10 11 weak expression: see section 04

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T29
Entity Detected:	Slc25a31, ( MGI:1920583)
Sequence:	sense strand is shown >T29 TCCNNACANCTGTGGCGCCCATCGAGCGTGTGAAGCTGCTGCTGCAGGTGCAGGCGTCCTCCAAGCAGAT AAGCCCTGAGGCGCGCTACAAGGGCATGCTGGACTGCCTGGTGCGCATTCCTCGTGAGCAAGGATTTTTA AGTTATTGGCGTGGCAATTTGGCAAATGTTATTCGATACTTTCCAACACAAGCCTTAAACTTCGCTTTTA AGGACAAATACAAAGAACTTTTCATGTCTGGTGTTAATAAAGAAAAACAGTTCTGGAGATGGTTTCTAGC AAACCTGGCTTCTGGAGGGGCTGCTGGAGCAACATCCTTGTGTGTAGTATACCCACTAGATTTTGCCAGA ACCCGATTAGGTGTTGATATTGGAAAAGGTCCTGAGCAGCGGCAGTTCACGGGTTTGGGTGACTGCATTA TGAAAATAGCCAAGTCAGATGGACTGATTGGTCTATACCAAGGGTTTGGTGTCTCTGTTCAGGGTATCAT TGTTTACCGAGCCTCTTACTTTGGAGCTTATGACACCGT
Notes:	The probe template was PCR amplified from IMAGE:944172 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:944172 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008163 same experiment
	EMAGE:31675 same embryo
	EMAGE:29940 same embryo
	EMAGE:31760 same embryo
	EMAGE:31683 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source