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EMAGE:31699

Gtf2b general transcription factor IIB ( MGI:2385191)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
testis
moderate moderate
regionalmoderate expression: see section 06 07 08 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T233
Entity Detected:Gtf2b, general transcription factor IIB ( MGI:2385191)
Sequence:sense strand is shown

>T233
CGGAGCTGTTGGCGGAGCCGCGAAGATGGCGTCGACCAGCCGTTTGGATGCTCTCCCAAGAGTCACATGT
CCGAATCATCCAGATGCAATTTTAGTTGAAGACTACAGAGCTGGCGATATGATCTGTCCCGAATGTGGCC
TAGTTGTAGGGGACCGAGTTATTGATGTGGGATCTGAATGGAGAACTTTCAGCAATGACAAAGCAACAAA
AGACCCATCTAGAGTTGGAGACTCTCAGAATCCTCTTCTGAGTGATGGAGATTTGTCCACCATGATTGGC
AAGGGTACAGGAGCTGCAAGTTTTGATGAGTTTGGCAATTCTAAGTATCAGAACCGGAGAACAATGAGTA
GCTCTGATCGAGCAATGATGAACGCATTCAAGGAAATCACGACCATGGCGGACAGAATCAACCTCCCTCG
CAATATAGTTGATCGAACAAATAATTTATTCAAGCAAGTGTATGAGCAGAAGAGCCTGAAGGGCAGAGCT
AATGACGCGA
Notes:The probe template was PCR amplified from IMAGE:2650779 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2650779 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:NMRI
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_008180 same experiment
 EMAGE:31281 same embryo
 EMAGE:31291 same embryo
 EMAGE:31290 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS