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EMAGE:31813

PKHD1 (PKHD1)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813
euxassay_006196_01 euxassay_006196_02 euxassay_006196_03 euxassay_006196_04 euxassay_006196_05
EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813
euxassay_006196_06 euxassay_006196_07 euxassay_006196_08 euxassay_006196_09 euxassay_006196_10
EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813
euxassay_006196_11 euxassay_006196_12 euxassay_006196_13 euxassay_006196_14 euxassay_006196_15
EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813 EMAGE:31813
euxassay_006196_16 euxassay_006196_17 euxassay_006196_18 euxassay_006196_19 euxassay_006196_20
EMAGE:31813 EMAGE:31813
euxassay_006196_21 euxassay_006196_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
diaphragm
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 17 18 19 20 21 22
pancreas
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 13 14 15 16
vertebral axis musculature
moderate moderate
spottedmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
kidney calyx
strong strong
regionalstrong expression: see section 01 moderate expression: see section 02 03 04 05 06 07 08 13 14 15 16 17 18
tongue muscle
weak weak
spottedweak expression: see section 11 12 13 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T490
Entity Detected:PKHD1, (PKHD1)
Sequence:sense strand is shown

>T490
GGCCTGTCACCGAATAGCGCGGCTTCGTTTAGAAGGGGACGGTTTCTCTCATTTGAGGCACAAGGCTGAC
ACAGCATCAGGCACAAAAACTACATGGCACAAATCAACCCTAAAAATGCTTCTAATATCTGAGGAATCTC
AAGAGAGCTGAGCTAACTGGACATGATGCTTGCCTGGCTGGTCTCTCTGCTGAGTATGGAAGTACTACTG
TTAGCAAAGCCTTACTCGAGTTTCCAGTTTGAACCCGCAGAAGGTAGCCTTGCAGGAGGAACATGGATCA
CAGTTGTATTTGACGGTTTGGATAGAAGTATTCTTTACCCCAACAATGGCTCTCAGCTGCAGATAGACCT
GGTGAGTGTGGCAATACCTACCTTGAGGATCCCCTGTGACGTCTCTCCGGCCTTCGTGGATTTGCCTGTG
GTGACATGCCAGACGAGGTCTCTTCCATCAGAAGCAGATGCAGGGCCATACTCCCTGGAAATGCGCTCTG
GGGAGCAAGTG
Notes:The probe template was PCR amplified from IMAGE:1481154 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1481154 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006196 same experiment
 EMAGE:31901 same embryo
 EMAGE:31824 same embryo
 EMAGE:31781 same embryo
 EMAGE:30918 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS