EMAGE mouse gene expression spatial database

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EMAGE:31839

D12Ertd647e ( MGI:1277180)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001958_01	euxassay_001958_02	euxassay_001958_03	euxassay_001958_04	euxassay_001958_05

euxassay_001958_06	euxassay_001958_07	euxassay_001958_08	euxassay_001958_09	euxassay_001958_10

euxassay_001958_11	euxassay_001958_12	euxassay_001958_13	euxassay_001958_14	euxassay_001958_15

euxassay_001958_16	euxassay_001958_17	euxassay_001958_18	euxassay_001958_19	euxassay_001958_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

weak

regional

weak expression: see section 02 03 04 10 11

testis

moderate

regional

moderate expression: see section 01 02 03 04 13 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T741
Entity Detected:	D12Ertd647e, ( MGI:1277180)
Sequence:	sense strand is shown >T741 TCCTCGAGNCTGTTGGCCTACTGGTTATTTCAGCAGCTGTCTTCAGTCACTGGGTGTGTCTTCCTGCACA GTGGACTTGACGGGCNTGGAGGGAGGAGAGGAAATTGAAACCTATCTCATCCTCTAGTTTATTTACATCA TTGGATTCGGTTCCTGTTTGGAAACTTAACTGGTCCTCATGGCGTTTTCGGGCACAGGGACACTGGTGGC TTCCATTGTCTCCAAGATGACATCTTCAGCAGCCATGGTCAAAGCTGGAGGGGCAGTCTCTGGCACCATT CTAGCTGGTTCGAAGGGGCTCATCCTGTTAACACAGAGTGCCCTGGGCTCTGCAACATCTGCACTTGGAG CACTGAAGGTTGGCACCATTTTATCTGGGTTCTCTGCCTCCACCTTGGCTGCTTCCCCCATTGGAGAGGA ACCTGCTTTGGTGGGCAGGGAGAAGAAAAGTGACTTCAGATTTTCCTTCCCCGCAGCAGGCAAAGCAGCA AGAAGGCAGAGAAAGGAAGGACAAAGCAAAATCTACCTGAAGGGTTTTGAGTTCTCCAGAGCCAAGGCTG TTGTGGC
Notes:	The probe template was PCR amplified from IMAGE:1890890 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1890890 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001958 same experiment
	EMAGE:31565 same embryo
	EMAGE:31399 same embryo
	EMAGE:31870 same embryo
	EMAGE:31766 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source