EMAGE mouse gene expression spatial database

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EMAGE:31852

Chordc1 ( MGI:1914167)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005070_01	euxassay_005070_02	euxassay_005070_03	euxassay_005070_04	euxassay_005070_05

euxassay_005070_06	euxassay_005070_07	euxassay_005070_08	euxassay_005070_09	euxassay_005070_10

euxassay_005070_11	euxassay_005070_12	euxassay_005070_13	euxassay_005070_14	euxassay_005070_15

euxassay_005070_16	euxassay_005070_17	euxassay_005070_18	euxassay_005070_19	euxassay_005070_20

euxassay_005070_21	euxassay_005070_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vomeronasal organ

moderate

regional

moderate expression: see section 14

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 16 weak expression: see section 09 10 11 12 17 18

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7476
Entity Detected:	Chordc1, ( MGI:1914167)
Sequence:	sense strand is shown >T7476 GACTAGTTCTAGTCCTGAAGCAGTACCGGCGGCTTCCGGAAGCTAGCTGCGGCCTGAACAGTAGTGCTGG TGATTGCTGCGGTGATTCACTTCTAGTCCCGGGTGGTGTGGATTTCGCCGAGAAGACGGAATGGCGCTCT TGTGTTACAACCGCGGCTGTGGCCAGCGTTTCGACCCAGAGGCCAACTCGGACGATGCTTGCACATACCA CCCAGGTGTTCCTGTCTTTCATGATGCCCTAAAGGGTTGGTCTTGCTGTAAAAGAAGGACAACTGATTTC TCTGACTTCTTAAGCATTGTAGGCTGTACAAAAGGTAGGCATAATAGTGAGAAGCCACCTGAACCGGTCA AGCCTGAAGTCAAGACTACTGAGAAGAAGGAACTATCTGAATTAAAACCCAAATTTCAGGAGCACATCAT TCAAGCCCCTAAGCCAGTAGAAGCAATAAAAAGGCCAAGCCCAGATGAGCCAATGACAAACTTGGAGTTA AAAATATCTGCTTCCCTAAAACAAGCACTTGATAAACTTAAACTGTCATCTGGGAGTGAAGAAGATAAAA AAGAAGAAGACAGTGATGAAATTAAGATTGGGACTTCCTGTAAAAATGGAGGGTGTTCAAAGACATACCA GGGTCTACAGAGTCTAGAAGAAGTCTGTGTATATCATTCTGGAGTACCTATTTTCCACGAGGGGATGAAA TATTGGAGCTGTTGTAGAAGAAAAACTTCAGATTTTAATACATTCTTAGCCCAAGAGGGCTGTACAAGAG GGAAGCATGTATGGACTAAAAAAGATGCAGGGAAAAAAGTTGTTCCGTGTAGACACGACTGGCATCAGAC TGGGGGTGAAGTTACCATCTCAGTGTATGCTAAAAATTCCCTGCCAGAACTCAGTCAAGTAGAAGCCAAC AGTACTTTGTTAAATGTACACATTGTATTTGAAGGAGAGAAAGAATTTCATCAAAATGTGAAACTGTGGG GTGTAATTGATGTTAAACGAAGTTATGTAACTATGACTGCAACAAAGATTGAGATCACTATGAGAAAGGC GGAACCCATGCAGTGGGCAAGTCTTGAACTACCTACAACCAAAAAGCAGGAGAAACAAAAAGATATAGCT GATTGATTAGAAGAGAAATGGAATTCTTAATGTGTGTGGTGAAATAGTTGCTTGCTGCTGTAATCTTTTT GTTGTTTTTAGTCTGTCGTTTTGGGGTCATCATGAGGTTCTTCAAAGCCAAAGTCTGTTAAATGTTTGTG CCTCTATCTTCCTTTCGAGTTGCATAATATAATTATTCAAGTCTCCTCACTAGAACTATAGTTGTTGCCC TTACACTTGAAGGGAGGGAAAGAAACCATAAGTATAATTGCAGTATTTTTTAGTATAAATTATTATAAGC AGGAAGTGAAAGAAGTTTTGTTTTGTTTTGTTTAATCCTGATAACTCCTTTTTCCCTTAAATGGGAAAGC AAAGTGGTATATAAAAAACTGAGTTGGCCTCTCCATGAACTTTTGTTATTATAATTCTTGAAAATCTTTA AGGAAAAGACTTTTTGTTTCTTCCTATGCAGTAGTTACTTTTTGTAGTACTAATTCTGAAGTGGAGTCAA ACCGTCTGTGTGTGCTGGCTGAGTTTATAATTCATTTGCAGTTAAATTAATTTTAGAAAGTTGCTTCAAT ACTTGCATTACCTGGCAAGCAGTATAATTTAAGTCTATAGGAAATAAACTAAGATTATAGAATTACAATT TGGTTAAAATTCATCATAAGAGTAAGCAATAATGTTAAAATCTCTTTCCTGATTATTTAAAGAACTCTTT ATGGTGTTTTGACAAAATTATATCTGATTTATAGTCTAAACACTCTTATGGGTGATATCTGTCTCCCTGA TGAAAAACTGTATCTGTTAAATTGTGAATGTGTAGCTTCGTTGAAATACATTAAGGTGTGTGTTGCGGCT TGAATCTGAAATGTACCTGGAGGCTTGTTTTGAGCTGTTGGTCTTTCAACTTAGGGCTCTATTTTGAAGG CTGTGAAACCTTTAGCATATTGAGGGGTGAGCCTTTAAGGATTATACAGTCTTGCTGGTCTGATCCAGTC AGGACATATGAACCACCTCTGTCCCATACTCCCACTGCTGCCTCCTCAGCCATTATAGATTGTATCCTCT GAAACCGTGAGCCAAAATAAACCTTACCTCCCTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4236535 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4236535 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005070 same experiment
	EMAGE:30593 same embryo
	EMAGE:31032 same embryo
	EMAGE:30408 same embryo
	EMAGE:30410 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source