EMAGE mouse gene expression spatial database

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EMAGE:31857

Mkrn3 ( MGI:2181178)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006166_01	euxassay_006166_02	euxassay_006166_03	euxassay_006166_04	euxassay_006166_05

euxassay_006166_06	euxassay_006166_07	euxassay_006166_08	euxassay_006166_09	euxassay_006166_10

euxassay_006166_11	euxassay_006166_12	euxassay_006166_13	euxassay_006166_14	euxassay_006166_15

euxassay_006166_16	euxassay_006166_17	euxassay_006166_18	euxassay_006166_19	euxassay_006166_20

euxassay_006166_21	euxassay_006166_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

neural retina

strong

regional

strong expression: see section 01 02 03 20 21 22

vestibulocochlear viii ganglion

strong

homogeneous

strong expression: see section 05 06 07 16 17 18

stomach

moderate

spotted

moderate expression: see section 01 02 03 04 05 06 07

cervico-thoracic ganglion

strong

regional

strong expression: see section 06 07 11 12 13

tongue

strong

spotted

strong expression: see section 12

spinal cord

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

strong

homogeneous

strong expression: see section 06 16 17

trigeminal v nerve

strong

homogeneous

strong expression: see section 08 15

thoracic ganglion

strong

regional

strong expression: see section 08 09 10 11 12

midgut

moderate

spotted

moderate expression: see section 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16

facial vii ganglion

strong

homogeneous

strong expression: see section 04 05 06 17 18 19

not examined

regional

not examined expression: see section 01 02 03 04 20 21 22

vagus x ganglion

strong

homogeneous

strong expression: see section 06 16

trigeminal v ganglion

strong

homogeneous

strong expression: see section 03 04 05 06 07 16 17 18 19 20 21

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17

cervical ganglion

strong

regional

strong expression: see section 06 07 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8890
Entity Detected:	Mkrn3, ( MGI:2181178)
Sequence:	sense strand is shown >T8890 CGAGAAGAAGCTGGTCGAGCCATCTCCAGCTCCGCAATTCAAGCACAAAAAAGATATAATATTCGGGTTT TTGGACAGTGTTAGAGGCACGCTGTAAGATGGAAGAGTCTACAGCTCCCATTGAGGCCCACGCGGCCGCC GGCGCCGAGGCAGGTGCTGAAGGGGGGGAGGGTGTGTCTGTTCCCCCCCCTCCTCAGTTTGAGGCTGCTG GAGCTTCTGCGGGAGTCAGCTCGGCCCCTCTGCAGCAGGCATCGGGTCTGGCACCGCTCCTGGTGACTCC AGGCCCAGCGATCCGGCGCGCGGCGAGTTTGAGGCCAGCGCCAGCCGAAGGGGGCGGGGCCCGGTCTGGT CCGGAGCGCAACAGTGGCAGCTGGACGAAGCAAATCCTCTGCAGGTATTATCTGCATGGGCAGTGCAAGG AGGGCGATAACTGTCGCTACTCTCACGACCTTTCTGGGCGGCGGAGATCTAGGGGGGGGCAAGATGCTCA GCCTCGGGCCTCTGCAGACAGAGGACCCAAGATGGCCACTCGCTGGGAGCCCCCGACTCAGGAAGTGGCG GAAGCCCCCCCTGCTGCATCCTCAAGCTCCTTGCCTCTGATTGGCTCGGCTGCTGAAAGGGGCTTCACTG AAGCTGAGATTGACAATGCAGGCATTCGCTCAGCTGCTGAAAGGGGCTTCTCTGAAGCCGAGATTGACAA TGCAAGCCTTGCAGCAGGTGCTGCAGCGGGAGCGGGTGCAGAAGGCTGGGAGGGGGCAATTGAATTTGTT CCTGGACAGCCTTACCGAGGTCGCATGGTCCCCCCGCATGGTCCCGAGGCTCCTCTGCAGAGCCCGGCAA TTGAGAGAGAGCATATGGCTATGGGCATGGGGATGCCGATGCCAGTGCCAATGCCAATGCCAGTGCCAAT GCCAGTGCCAATGCCGCTGCCGCTTTGCCGCTATGCTGCCCGGGGACAATGCCTCCGTGGGGACAGGTGT GCATACCCCCATGGAGAAATATGCGACATGTGCGGGCAGCAGGCCTTGCACCCTTGGGATGCAGCTCAGC AGGAAGCTCATAGAAGGGCCTGCGTTGAAGCACACGAGAGAGATATGGAACTCTCTTTTGCTGTGCAGCG CAGCATGGACAAAGTGTGTGGCATCTGCATGGAGGTGGTCTATGAGAAAGCTGACCCCAGCGACCGCCGC TTTGGCATTCTTTTCAGCTGCAACCACACCTACTGTCTTAGGTGTATCCGCAGGTGGAGAAGTGCCACAC AGTTTGAGAACAGGATCAGTAAGTCCTGCCCACAGTGCAGGGTCTCCTCTGGCTTTGTCATTCCTAGTGA GTTTTGGGTGGAGGAGGAAGAGGAGAAGGAGAAACTTGTTCAGCAGTATAAGGAGGGGATGAGCCAGAAA GCGTGCAGGTATTTTGCTGGAGGCCTAGGTCACTGCCCATTTGGAGAATTCTGTTTTTACAAGCATGAAT ACCCCGAGGGCTGGAGAGATCAGCCTCCAAGACCAGATGGTGGTGGATCATCAAGCGCATACTGGCATCA AGTTTTGGAGCCTGTGCAGTTGCGAGAGGGAAACGTGCTGTTTAAAAGCCGTAAAAAAGAGCATTCTGTG CTTCGCCTGGCCAATCAGTTGCTTAAGAAGTTGCTTTGCCTGAGAGGCAGTTCTTCCTTCTCTGATGACC GTTGGCTCTTGCTTCAGTACCAGCTGGAAGAATATTTCAGTTTGAATCTGTAGTATCACGCTGTGGCATG TGGTCTGGTCTGCTAAAGCTCTGTTGTCTGCTGTTGCTTATTCCCCTTTATTGACTGAAAAACCCTATCG CTTTCAAAGGCTACTGAGGCAATCTTTATGCAGTATTGCTGTTTTCTTCTCTATTTCCTATTTCTTCTTG CTAGGATTGTGATGTTTCTCTGTGTTACAAAATAACAAGAAATCTTATAAGTATTGTTTGGTGGAATTAA TATTTCTGTCAACTGATGACTTGTGATGTCTTTTTTCCAACAGGATTCAATCACTTCTAGTCTAGTGTTT ACAGTTTCTCATTGTGAAGTCCCTTAAGAGTAAATGTGACAAGGTGACAAAAGTCTTTAACTGAATTATG AGCTTTTTGCTATATAGCCTTAAAAAAAATGGACCCTTACAGGACCTTGCAGCTGCTGTCTGTGTTTGTT TACATGTTTGTTTCTTCCCTTCTCTTTCAAGTGCACTTGTTAACTTGTGGTTACTTTGTGGTTCTGTTTT ACTTGACCAAAATGAGGTGCATATGTTTACATGTTTTTATCCTGTTTGGCTTGATGTGAAATTATTTATA TTTGGAAATATATATTTAAGAATGATATATATGCGTATATGTATACAAAGATGTATTTGAAAAAAACTGG AAATAAATATACACCATAATATATATGTTTGAAGTATTGAAATAAATGCAGAACTGAAAGCCCAGTGTTA AAGTCCAAGTGTGTGGGCTGTATGTGAATTGTGTGGATAATCTGCCCCTGCACTGATGTCAGTGATTAGC ATCTAATAAAAATGACTGCTAAGTGTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6415520 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6415520 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006166 same experiment
	EMAGE:29580 same embryo
	EMAGE:29751 same embryo
	EMAGE:29971 same embryo
	EMAGE:29742 same embryo
	EMAGE:30891 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source