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EMAGE:31867

C4bp ( MGI:88229)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867
euxassay_000871_01 euxassay_000871_02 euxassay_000871_03 euxassay_000871_04 euxassay_000871_05
EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867
euxassay_000871_06 euxassay_000871_07 euxassay_000871_08 euxassay_000871_09 euxassay_000871_10
EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867
euxassay_000871_11 euxassay_000871_12 euxassay_000871_13 euxassay_000871_14 euxassay_000871_15
EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867 EMAGE:31867
euxassay_000871_16 euxassay_000871_17 euxassay_000871_18 euxassay_000871_19 euxassay_000871_20
EMAGE:31867
euxassay_000871_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
kidney pelvis
moderate moderate
regionalmoderate expression: see section 12
foregut-midgut junction
weak weak
regionalweak expression: see section 01 18
kidney calyx
moderate moderate
regionalmoderate expression: see section 12 13 weak expression: see section 09 10 11 18 19 20 21
midgut
weak weak
regionalweak expression: see section 01 02 12 13 14 15 16 17 18
midgut loop
moderate moderate
regionalmoderate expression: see section 12 13 14 15 16 weak expression: see section 17 18
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T912
Entity Detected:C4bp, ( MGI:88229)
Sequence:sense strand is shown

>T912
TCCTCGAGNCTGTTGGCCTACTGGAACTGCTTAACCAGAAACTTGTGTGGGTGGAATGCAAGGATCACTT
GCGTGGGTAGACTTCAGAAGCTTTTTTGATGGAGCAAGTACTGGCCAGCTTTAGGATAATATGATCAACT
CTCATCTTGGGAGAAACTAGTACAGTAGAGATCCAAGTACCACCACAGAGTCTTTCTCTGCTATTGCAGG
TTTCCTGGCAGAACAAATGTGTGCAAAGCAGCAGCAGACCCTGCTCCCCACAAGAGCTGCACATGGGAGG
CTTCATAGAAACAGAGATGCTGTGGCCTGGCCCTTTTCTACGCTCTGCAGAGTCTCTGGCCCAACTTTGT
TCCAAATGACCTTTACTGCTGCTCTTTGGGTTGCTGTATTTGGCAAATGTGGTCCACCACCTGCTATACC
CAATGCCCTGCCAGCAAGTGACGTGAATCGGACAGACTTCGAAAGTCACACCACCCTGGAATACGAATGC
CTCCCTGGCTATGGTAGGGGAATATCAAGGATGATGGTTTACTGCAAACCTTCG
Notes:The probe template was PCR amplified from IMAGE:1924716 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1924716 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000871 same experiment
 EMAGE:31516 same embryo
 EMAGE:31834 same embryo
 EMAGE:31840 same embryo
 EMAGE:30057 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS