EMAGE mouse gene expression spatial database

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EMAGE:31886

Fmo2 ( MGI:1916776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003906_01	euxassay_003906_02	euxassay_003906_03	euxassay_003906_04	euxassay_003906_05

euxassay_003906_06	euxassay_003906_07	euxassay_003906_08	euxassay_003906_09	euxassay_003906_10

euxassay_003906_11	euxassay_003906_12	euxassay_003906_13	euxassay_003906_14	euxassay_003906_15

euxassay_003906_16	euxassay_003906_17	euxassay_003906_18	euxassay_003906_19	euxassay_003906_20

euxassay_003906_21	euxassay_003906_22	euxassay_003906_23	euxassay_003906_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 10

greater sac mesothelium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 18 19 20 21 22 23 24 not examined expression: see section 17

foot

moderate

regional

moderate expression: see section 06 07 08 21 22 23 24

pancreas

moderate

regional

moderate expression: see section 08 09 13 14 15

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 14

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 13 15 16 17 moderate expression: see section 06

mesothelium

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

hand

moderate

regional

moderate expression: see section 01 02 03 20 23 24

pericardial cavity

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

omental bursa mesothelium

moderate

regional

moderate expression: see section 17 not examined expression: see section 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6988
Entity Detected:	Fmo2, ( MGI:1916776)
Sequence:	sense strand is shown >T6988 CTCTTCTGCCAAGAGGACAGTGCTAAGAAGCACAAATAAAGGAGACAATGGCAAAGAAGGTTGTGGTGAT TGGAGCAGGGGTCAGTGGCCTAATCTCTCTGAAGTGCTGCGTGGACGAGGGTCTGGAGCCCACCTGCTTC GAAAGGACTGAAGATATAGGGGGACTGTGGAGATTCAAAGAAAACGTGGAAGATGGCCGCGCTAGCATCT ACCGCTCTGTCATTACCAACACTAGCAAAGAAATGTCCTGTTTCAGTGACTTCCCGATGCCGGAGGACTT CCCCAACTTCCTGCACAACTCTAAACTCCTGGAATATTTCAGGATCTTTGCCAAGAAATTTGATCTCCTA AAATATATTCAGTTTCAGACAACTGTCATTAGTGTGAAAAAACGCCCGGATTTCGCATCTTCTGGGCAAT GGGAAGTTTATACTCAGAGCAACGGAAAGGAGCAACGCACTGTCTTTGACGCTGTTATGGTTTGCAGCGG CCATCACATCCAGCCTCACCTGCCACTCAAGTCATTCCCAGGTATTGAGAGGTTCCGAGGCCAGTATTTC CATAGCCGCGAATACAAGCACCCAGTGGGGTTTGAGGGGAAACGCATCCTGGTGGTTGGAATAGGAAATT CAGCTGCAGATATTGCCTCTGAACTGAGTAAGACGGCCGCACAGGTGTTTGTCAGCACCAGACATGGATC CTGGGTCATGAGTCGAATCTCTGAAGATGGCTACCCTTGGGACATGGTGTTCCACACTAGATTTAGCTCC ATGCTCCGAAATGTCCTGCCCCGCACGGTCGTCAAGTGGATGATGGAACAACAGATGAATCGATGGTTCA ACCATGAAAATTATGGTCTGGTGCCTCAGAACAAATACCTTATGAAAGAACCTGTGCTAAACGACGACCT CCCCAGCCGCCTGCTCTACGGAGCCATCAAAGTAAAAACACGGGTGAAGGAGCTCACGGAGACAGCTGTG GTCTTCGAGGATGGCACGGTGGAGGAAGACGTGGATATCATTGTCTTTGCCACGGGGTATACGTTCTCTT TTTCTTTCCTGGAAGACTCACTTGTTAAAGTGGAGGATAATAGGGTTTCCCTGTACAAAGCCATGTTTCC CCCTCACCTGGAGAAGCCAACCCTTGCGTGCATAGGTCTCATCCAGCCTCTAGGCTCCATCTTCCCAACC GTAGAGCTTCAGGCGCGCTGGGCAACAAGAGTTTTCAAAGGCTTGTGTAGCTTGCCTTCGGAGACGACTA TGATGGCAGACATTGTTGAAAGAAATGAAAAAAGAGTTAACCTGTTTGGGAAAAGCCAGAGTCAGATACT GCAGACCAACTACGTTGACTACCTGGATGAACTCGCCTTGGAGATAGGTGCAAAGCCAGATTTCGTCTCT CTCTTTTTCAAAGACCCTAAACTGGCTGTGAAACTCTACTTCGGACCCTGTAATTCCTACCAGTACCGCC TGGTTGGACCTGGGCAGTGGGAAGGAGCCAGGAACGCCATCCTCACCCAGAAGCAGAGGATCCTGAAACC CTTAAAGACCCGGACTCTCCAGTCCTCTGATAGTGCCCCAGTGTCTTTCCTGCTCAAGATCCTGGGGCTG CTCGCTGTTGTTCTGGCCTTCTTTTTCCAACTTCAGGGGTTTTAATCAGTCACATGACAGTTTTCTTCAT CACTGCCTCTTTGAAAGGGAAACTGGAGAAATTCCATCCTCATTATAATTTTACTTTAGGGGAATAAGTA GGGGGACTAATTGTAGGGAGATCAGAAAACTTAGGTCTAGATATAAAACTAAGTTAAGGTTACAGGATGT TCTTATCATTGTTTCCCCAAATTCATCAGTCTCCACATGGTATTCTATAATAACACCAGCACAGGTAGAT AGTACTATCAGCCATGGCAGGTTTTGAGTTACAAGATTTAAAAAAAATCAATAAAATTATGTAGAATAAA AAGTAAGTCATAGTTGAGCATGTTAGAACATGGCTTTAATCTTAGCACTTGGAAGGCAGATGGATCTCTG TGAGTTCAAAGAAAAAAAAAGGCAAGACACTGAAGAACTGAAATTGTAGTAGATATTTCACATCTTGTAC ACTATGATGCTCAGTGCTGTTCCTATGCATACTCTGGTCACAGTTCCCACTTCTGATGCAAAAATGAAAA CAATATTCCCTAGCTACAACATATAGATAGATACTGTTTCTCATAGACTAGACATTGTGCTGTGTGTACA TTATGTATTTTATTGGATTTAATTCTCACAGCAACTGTGTGATGGGTAGAGCTATATATATCACCCATTC TACAGATGGCACTGAGGAGGGGACATTATAGAGCATGTCCTAAACAGCTGGTCTTGAGATGAGGCTTCAA TTGAAGCCCAATTTGTTAAACTCAGGAACTTATGCTCATGACCTCCCCATGCAATATGCTTATTAATGAA TAGTCCACTCTTGACGAAGTACTTCCTAATCACTTATTTTAGTCATTATAAACTCACACAATAGCTCTAT TCATTAGCATAAAGTTTTCTAAGACTATTGTAGAAAATGGCACTTTCTTTGCAAATAGAATCTGTCTAGG TTCATTTACAATATTAGGTCTGTATGTCCGCTGGAATTTAAAAAGCACTTGGGATAAGCACAGTAGTACA GACCTTTAATCCCAGCACTAAGGAGTCAAGACAGGCAGATTACTACCAGTTTGAGGCTGGGTTGCTCTAC ATAGCAAAATCTGGGCCAGCCAGGGCTACATGGTGACAACCTGTCTCAAAAGAAAAATAATTACAAAGAG ATTTAGTATTAAAAATAGCTATGCAGTGATATTTAATCATCACCATGTGAAAGTATAAGAAATGAAGTGA AGTGCATCTCCCAAGCAGATGAAACTCTACAATGGCTGAGGTTATAGGGTTATAGGGTTAACATTATAGG TAAGAAAAGCAAATCTCAGCAATGTCCTGTCTTTGTCATCTGATAAGGGGCAGGGGGAGGTTGTCAGTTA TGAGAGACATTTTCTTGGAACGGAAAACTAAAGGAGACACCAGCAGTTTAGTGTCAACAAACATTGAGCA ACATAGCAAACACCAGGCTACAAACACTCCTGATAGCATCTCTTTCCATGAACCGCACTGCAAAAGATAA GCTATGCCCAGAGACCAGACCAGAGCCTCTGCAGAGGGGACAAAGGCCAGGATACTGAAGGTTTAAGTAA ATAGCTCTTCTTTAGCCCTCCGAGCAGTGGTTCTCAGTTTTCCTAATGCTACACCCCCTTAAAACAATCC CTCATGTTGTGGTGACCCTCAACCATAAAAGTATCTCATTGCTGCTTCATAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3990305 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3990305 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003906 same experiment
	EMAGE:29439 same embryo
	EMAGE:31244 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source