EMAGE mouse gene expression spatial database

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EMAGE:31888

Stra8 ( MGI:107917)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011751_01	euxassay_011751_02	euxassay_011751_03	euxassay_011751_04	euxassay_011751_05

euxassay_011751_06	euxassay_011751_07	euxassay_011751_08	euxassay_011751_09	euxassay_011751_10

euxassay_011751_11	euxassay_011751_12	euxassay_011751_13	euxassay_011751_14	euxassay_011751_15

euxassay_011751_16	euxassay_011751_17	euxassay_011751_18	euxassay_011751_19	euxassay_011751_20

euxassay_011751_21	euxassay_011751_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

homogeneous

moderate expression: see section 06 07 08 11 12 13 14 15 16 17

facial vii ganglion

moderate

homogeneous

moderate expression: see section 05 06 19

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21

spleen primordium

strong

regional

strong expression: see section 02 03 04

spinal cord

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13 14 15 16

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

homogeneous

moderate expression: see section 07

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37092
Entity Detected:	Stra8, ( MGI:107917)
Sequence:	sense strand is shown >T37092 CAGCTTAGAGGAGGTCAAGGAAGAATATGCCAGAATGTATTCCGAGAATGACAGTGTATTCCTAAACAGT TTTCTTCAGGACAGTCCCCCTGAGTGGTTCCCCTCTGAGGCTGTTGGACCAGATGCTGAAGAAGAAGGAG AAGAAGAAGGAGAAGAAGAAGGAGAAGAAGGAGAAGAAGAAGAGGAAGGAGACGAAGAAGGAGAAGAAGA AGAAGAAAACGGTGAAGAGAGAGAGGTAGAGGAGTACCAGGAAGAGGAAGAAGAAGAAGAGGAGGAGGAG AAAAAAGTCGATCTCTCCCACTCCTCCTCCACTCTGTTGCCGGACCTCATGGAATTTGAACGGTATCTCA ACTTTTACAAGCAGACCATGGACCTCCTGACCATGAACAGCATCATCTCTGCACATGAAGTGACACTTCC TATTGTCTCTGCCGCCATCTCCCACCTGTGGCAGACTCTCTCTGAGGAGAAAAAGGCCAGACTCCTGCAG GTGTGGGAACAGCAGCACAGCGCCTTCGCAGACCTCACCGAGGCCTGTCTAGAGCTGGCCGGGGTGGAGG GCAGCATGAAGGACAGCGGCGTGGACAGCCAGGGAGCGAGCTGCTCGCTGGAGTCCACCCCAGAAGAGAT CCTTTTTGAAGATGCTTTTGACGTGGCAAGTTTCCTGGACAAGAGTGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74162. Forward Primer - name:074162_F_cDNA_Stra8, sequence:CAGCTTAGAGGAGGTCAAGGAA; Reverse Primer - name:074162_N_SP6_cDNA_Stra8, sequence:TCACTCTTGTCCAGGAAACTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_011751 same experiment
	EMAGE:30428 same embryo
	EMAGE:30614 same embryo
	EMAGE:31499 same embryo
	EMAGE:31536 same embryo
	EMAGE:30411 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source