EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31937

Pcbp4 ( MGI:1890471)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004531_01	euxassay_004531_02	euxassay_004531_03	euxassay_004531_04	euxassay_004531_05

euxassay_004531_06	euxassay_004531_07	euxassay_004531_08	euxassay_004531_09	euxassay_004531_10

euxassay_004531_11	euxassay_004531_12	euxassay_004531_13	euxassay_004531_14	euxassay_004531_15

euxassay_004531_16	euxassay_004531_17	euxassay_004531_18	euxassay_004531_19	euxassay_004531_20

euxassay_004531_21	euxassay_004531_22	euxassay_004531_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 10 11 12 13 14 15 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 03 21

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5773
Entity Detected:	Pcbp4, ( MGI:1890471)
Sequence:	sense strand is shown >T5773 GGACAATGTGGGACTGAGCGGCCCAGCCGCTGCCGCCGCCGCAGAACAGCCCCAGCGAGAGCTAGCTGAG CCACAGTGTGGACACACTCCCAGGCCGCTGCGGCCCCAGCCCCGCCTGACAGGATGAGCAGTTCAGATGC GGGGCTGGAGGAGGGGCCCGAGCTTAGTATCACCCTCACCCTGCGGATGCTGATGCACGGGAAGGAAGTG GGCAGCATCATTGGGAAGAAGGGAGAGACTGTAAAGCGAATCCGGGAACAGAGCAGTGCCCGGATCACCA TCTCGGAGGGCTCCTGCCCCGAGCGAATCACCACCATCACAGGCTCTACGGCTGCCGTCTTCCACGCGGT CTCCATGATTGCCTTCAAGCTGGATGAGGACCTTTGTGCTGCTCCTGCAAATGGTGGCAGTGTCTCCAGA CCTCCAGTGACCTTGCGCCTTGTCATCCCTGCCAGCCAGTGTGGATCACTGATTGGGAAGGCAGGCACCA AAATCAAGGAGATCCGAGAGACTACAGGAGCCCAGGTGCAGGTGGCAGGGGACCTGCTCCCAAATTCCAC AGAGCGTGCTGTCACTGTGTCCGGGGTGCCTGACGCCATCATCCTGTGTGTGCGCCAGATCTGTGCTGTT ATTCTGGAGTCCCCACCCAAAGGAGCCACAATCCCGTATCATCCGAGCCTCTCCCTAGGTACTGTCCTCC TCTCGGCCAACCAGGGTTTCTCTGTCCAGGGTCAGTATGGAGCAGTGACCCCTGCAGAGGTCACCAAGCT CCAGCAGCTCTCAGGCCACGCCGTTCCCTTTGCATCACCCAGTGTGGTGCCAGGACTGGATCCCAGCACA CAGACCAGCTCACAGGAGTTTCTGGTTCCCAATGATCTGATTGGCTGTGTGATCGGACGCCAGGGCAGCA AGATCAGCGAGATCCGGCAGATGTCGGGGGCACATATTAAGATCGGGAACCAAGCGGAGGGTGCTGGGGA GCGGCATGTGACCATCACTGGCTCACCGGTCTCCATCGCCCTGGCTCAGTACCTCATCACTGCCTGTCTA GAGACGGCCAAGTCTACCTCTGGGGGGACGCCTGGCTCAGCCCCCGCAGACCTGCCCACCCCTTTCTCGC CACCCCTGACGGCCCTGCCCACAGCTCCCCCAGGCCTGCTGGGCACACCTTATGCCATCTCCCTCTCCAA CTTCATCGGCCTCAAGCCTGTGCCCTTCCTGGCTCTACCACCTGCTTCCCCAGGGCCACCGCCGGGCTTG GCGGCCTACACTGCCAAGATGGCAGCGGCCAATGGGAGCAAGAAAGCTGAACGGCAGAAATTCTCCCCCT ACTGAGGCCGGCCGGCTGAGGCACCGGCAGGGGCAGGCAGGACCGCCGGCAGGGGCTGCCTCCACATCCT TCCTGCCCAAGGAGACTCCACCATGGGGTCTCAAGCGCCAGTAATGCCAGACGCATGGATGCACCCCCTA CCCTGCCCCATCTTTGGAGATCCTCCTCTGAGAGGGGACAGTTTCTGGCCCAGGGTTCTAGGAGTATGGC AGCCCCAGGGCAGGGGCCCCACCTCTACAGACCTATGCTTGGATGCAGAGAATGGCCAGGGGCTCTCTGG AGCCCTGCTGTGGGAGGGGTTACTCATGTCCCCTCCATCCCTTTGGGCTTCATCCTGCTGTCCCAGTGGG AATTGGAGGAGAGTGAGGGGCGGCCAGGGCCAAGCTTCTCCTCATCCCCTGTAGATCCTGCTGCTTCTAC TGATACCCTTTTGACTGGAATAGACTGGCTGGGCTTGTCCGGGGGAGGGCAACCCAAAGAGAGGGCTCTG CCTGCCTGTAGGTGTGGCCTGGGGGGCAGGGGCCCAGTCTCAGCAGCAGACACTCTGTACAGTTTTTTCA ATCCCTGTTTTTGAATAAATATTCTCAGCGACCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2654291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2654291 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004531 same experiment
	EMAGE:30645 same embryo
	EMAGE:31932 same embryo
	EMAGE:30643 same embryo
	EMAGE:31930 same embryo
	EMAGE:30631 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source