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EMAGE:31984

Ttll5 ( MGI:2443657)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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EMAGE:31984
euxassay_012593_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
choroid invagination
moderate moderate
regionalmoderate expression: see section 04 05 06 13 14 15 weak expression: see section 03 07 11 12
medulla oblongata part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 04 05 06 08 09 13 14 15 16 17 18 weak expression: see section 01 02 03 07 10 11 12 19
metencephalon part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 04 05 06 08 09 13 14 15 16 17 18 weak expression: see section 01 02 03 07 10 11 12 19
diencephalon roof plate
moderate moderate
regionalmoderate expression: see section 08 09 weak expression: see section 10
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31555
Entity Detected:Ttll5, ( MGI:2443657)
Sequence:sense strand is shown

>T31555
TGCAATGAAAGTTGCCGAGTGGACTAATAAAGGTGAACCGTGCTGCAAAATCGAGGCTCAGGAGCCAGAG
TCTAAATTCAACCTGATGCAGATTCTCCAGGACAATGGCAATCTAAGCAAAGTACAGGCCCGGTTAGCAT
TCTCTGCCTATCTTCAGCATGTGCAAATCCGTCTGACAAAAGACAGTGGTGGTCAGACACTCAGTCCCAG
CTGGGCTGCCAAAGAGGATGAACAGATGGAACTGGTGGTTCGTTTTCTCAAGCGAGCGTCAAGTAACCTC
CAGCACTCGCTGAGGATGGTATTGCCTAGTCGGCGGTTGGCACTTCTGGAGCGCAGGAGAATCCTTGCCC
ACCAGCTAGGAGACTTTATCGGTGTGTACAACAAGGAAACAGAACAAATGGCTGAAAAGAAATCAAAGAA
GAAACTTGAAGAAGAGGAAGAAGATGGGGTGAACGCAGAAAGCTTTCAGGAGTTTATTCGACAAGCAAGT
GAGGCTGAACTGGAGGAGGTGTTGACTTTTTATACCCAAAAAAACAAGTCTGCCAGTGTCTTCCTGGGGA
CTCACTCGAAAAGCTCTAAGAACAGCAGTAGTTACTCTGATAGTGGGGCAAAAGGTGATCACCCTGAGAC
CATACAAGAAGTGAAAATAAAGCAACCCAAACAGCAACAGGCAACAGAAATCCACGCCGATAAATTATCT
CGATTTACCACTTCATCAGGAAAAGAGGCGAAACTAGTGTATACCAATTGCTCTTCTTTCTGCAGTCCTG
CTGCTGTTCTTCTGCAGAGACTCCCCAGCTCCCATCTGTCGTCCGTGATCACAACCTCTGCCCTCTCGGC
AGGCCCTGGCCACCATGCTTCTCTATCTCAAATCCCTCCAGCTGTCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5357408), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 31969. Forward Primer - name:031969_F_IRAV67-70_O03_D630041K24Rik, sequence:TGCAATGAAAGTTGCCGA; Reverse Primer - name:031969_R_SP6_IRAV67-70_O03_D630041K24Rik, sequence:GGGACAGCTGGAGGGAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_012593 same experiment
 EMAGE:30659 same embryo
 EMAGE:30548 same embryo
 EMAGE:31982 same embryo
 EMAGE:31980 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS