EMAGE mouse gene expression spatial database

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EMAGE:32022

Rbpms RNA binding protein gene with multiple splicing ( MGI:1334446)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000586_01	euxassay_000586_02	euxassay_000586_03	euxassay_000586_04	euxassay_000586_05

euxassay_000586_06	euxassay_000586_07	euxassay_000586_08	euxassay_000586_09	euxassay_000586_10

euxassay_000586_11	euxassay_000586_12	euxassay_000586_13	euxassay_000586_14	euxassay_000586_15

euxassay_000586_16	euxassay_000586_17	euxassay_000586_18	euxassay_000586_19	euxassay_000586_20

euxassay_000586_21	euxassay_000586_22	euxassay_000586_23	euxassay_000586_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

weak

regional

weak expression: see section 13 14

neural retina

moderate

homogeneous

moderate expression: see section 01 02 22 23 24

hindgut

weak

homogeneous

weak expression: see section 12 13 14 15

foregut-midgut junction

weak

homogeneous

weak expression: see section 11 12 13 14 15 16 17 18 19 20

stomach

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10

midgut

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2128
Entity Detected:	Rbpms, RNA binding protein gene with multiple splicing ( MGI:1334446)
Sequence:	sense strand is shown >T2128 TGGCCTCGAGCCAGATTCGGACGAGGCCAACCCCGCGCCCCAGTCCCGCGCGGCGACGAAGGACCGGGAA GATGAACGGCGGCGGCAAAGCCGAGAAGGAGAACACCCCGAGCGAGGCCAACCTTCAGGAGGAGGAGGTC CGGACCCTATTTGTCAGCGGTCTGCCTCTGGACATCAAGCCCCGAGAGCTGTACCTGCTCTTCAGACCAT TCAAGGGCTATGAAGGTTCTCTCATAAAGCTCACATCTAAACAGCCCGTAGGCTTTGTCAGTTTTGACAG TCGCTCAGAAGCAGAGGCCGCAAAGAACGCTTTGAACGGCATCCGCTTCGATCCTGAAATCCCGCAAACG CTACGACTAGAGTTTGCTAAGGCAAACACGAAGATGGCCAAGAACAAACTCGTAGGGACTCCAAACCCCA GTACTCCTCTGCCCAACACTGTACCTCAGTTCATTGCCAGGGAGCCATATGAGCTCACAGTACCTGCACT TTACCCCAGTAGCCCTGAAGTTTGGGCCCCGTACCCTCTGTACCCAGCGGAGTTAGCGCCTGCTCTTCC
Notes:	The probe template was PCR amplified from IMAGE:870794 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:870794 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000586 same experiment
	EMAGE:30080 same embryo
	EMAGE:31515 same embryo
	EMAGE:31526 same embryo
	EMAGE:30186 same embryo
	EMAGE:31832 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source