EMAGE mouse gene expression spatial database

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EMAGE:32051

Pcyt1a ( MGI:88557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003868_01	euxassay_003868_02	euxassay_003868_03	euxassay_003868_04	euxassay_003868_05

euxassay_003868_06	euxassay_003868_07	euxassay_003868_08	euxassay_003868_09	euxassay_003868_10

euxassay_003868_11	euxassay_003868_12	euxassay_003868_13	euxassay_003868_14	euxassay_003868_15

euxassay_003868_16	euxassay_003868_17	euxassay_003868_18	euxassay_003868_19	euxassay_003868_20

euxassay_003868_21	euxassay_003868_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 weak expression: see section 05 06 07 08 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7240
Entity Detected:	Pcyt1a, ( MGI:88557)
Sequence:	sense strand is shown >T7240 GAAGATGGATGCACAGAGTTCAGCTAAAGTCAATTCGAGGAAGAGGAGGAAAGAGGCACCTGGCCCTAAT GGAGCAACAGAGGAAGATGGAATTCCTTCCAAAGTGCAGCGTTGCGCAGTTGGTTTACGGCAGCCAGCTC CTTTTTCTGATGAAATTGAAGTTGACTTTAGTAAGCCCTATGTCAGGGTGACTATGGAAGAAGCCTGCAG GGGAACTCCGTGTGAGCGGCCTGTGAGAGTTTATGCGGATGGAATATTTGACTTGTTTCACTCTGGTCAT GCCCGGGCTCTGATGCAAGCAAAGAACCTTTTCCCTAATACGTATCTAATTGTGGGAGTCTGCAGTGATG AGCTAACGCACAACTTCAAGGGCTTCACTGTGATGAACGAGAATGAGCGCTATGACGCCGTGCAGCACTG CCGCTACGTGGACGAGGTGGTGCGGAACGCCCCCTGGACTCTGACGCCTGAGTTCCTGGCAGAGCACCGG ATTGATTTTGTCGCCCATGACGACATCCCCTATTCTTCTGCAGGGAGCGATGATGTGTATAAGCACATCA AGGACGCAGGCATGTTTGCTCCCACACAGAGGACAGAAGGTATCTCCACATCAGACATCATCACCCGCAT TGTCCGTGACTATGATGTGTATGCAAGACGGAACCTACAGAGGGGCTACACTGCCAAGGAGCTCAATGTC AGCTTTATCAACGAGAAGAAATACCACTTGCAAGAACGAGTTGATAAAGTAAAGAAGAAAGTGAAAGATG TGGAGGAAAAGTCAAAAGAGTTTGTGCAGAAGGTGGAAGAGAAGAGCATCGACCTCATCCAGAAGTGGGA GGAGAAGTCCCGAGAGTTCATTGGAAGTTTCCTGGAAATGTTTGGTCCAGAAGGAGCACTGAAGCACATG CTGAAGGAGGGAAAAGGTCGGATGCTGCAGGCCATCAGTCCCAAGCAGAGCCCCAGCAGCAGCCCTACTC ATGAGCGCTCCCCCTCCCCCTCCTTTCGGTGGCCCTTCTCTGGCAAGACTTCCCCATCTTCCTCCCCAGC AAGTCTTTCTAGGTGCAGGGCTGTGACCTGTGACATCAGCGAGGATGAAGAGGACTAACTTTTCATCCCT GTTCCCTCTCTCCCCCACTGCCCATCACCTCCAGAAGCTCTCTGTCGAATTCCATTTTGTGATCCCAACA CTAAACCTAAGGACATCTACAAAGAAAAGACAACCGGGGCAAGAGGACCTGGGAATGGGGAACCAAAACA GCCCATGCCCTGAGACATTCTCAGCCACACCAAGGAGGCTGCCCATACCTTAGAAGCCTGCTCTGCATCC ATCTTCCCTCCCCAGAACTTTGCTTTACTGTTTATGCTCATGTAATCTTGACAGGGAAATTGTCATTTTT ATCAATTTTTTTTAAAAAGTAGTCTTTCAAATATAGTAAGTAGAACTAATTTTTTGCCCCTGAGGAGTGG GCAGAAGAAGGAAGCGTGGAATGCCCAGAGGCCTTTTTTCCTGAACACTAGTGCTTGCCTTCTGTTCTGA AGCAAAGTGGCTTGCCAGATCCTTCAAACAGGGAGCCATGCTAATGGCCAATTTGGGGACTATCATAATT TTAACTTCCCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4162053 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4162053 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003868 same experiment
	EMAGE:29826 same embryo
	EMAGE:29830 same embryo
	EMAGE:32059 same embryo
	EMAGE:32049 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source