EMAGE mouse gene expression spatial database

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EMAGE:32059

Igfbp1 ( MGI:96436)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003866_01	euxassay_003866_02	euxassay_003866_03	euxassay_003866_04	euxassay_003866_05

euxassay_003866_06	euxassay_003866_07	euxassay_003866_08	euxassay_003866_09	euxassay_003866_10

euxassay_003866_11	euxassay_003866_12	euxassay_003866_13	euxassay_003866_14	euxassay_003866_15

euxassay_003866_16	euxassay_003866_17	euxassay_003866_18	euxassay_003866_19	euxassay_003866_20

euxassay_003866_21	euxassay_003866_22	euxassay_003866_23	euxassay_003866_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12

liver right lobe

strong

regional

strong expression: see section 13 14 15 16 17 18 19 moderate expression: see section 20 21 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7241
Entity Detected:	Igfbp1, ( MGI:96436)
Sequence:	sense strand is shown >T7241 CCACGCGTCCGCGGACGCGTGGGCAGCCAGCATGGTCCACTGCCCGCGGAGACACACACCCAGCGAGCAT TGAACACTGCACACGGCCGTCTGCCCAGAGAGCTGTGACCACCATTGCCACTACTATCTACTCAGAAAGT CGTGACCACTGAGCAACTGCTGCCTGTCCAGAGTCTCATCCACAGCCTGCTGAACCAGTTGCGATGCCCG AGTTCCTAACTGTTGTTTCTTGGCCGTTCCTGATTCTCCTGTCCTTCCAGATTGGCGTGGCCGCTGGAGC CCCCCAGCCATGGCACTGTGCTCCCTGCACTGCTGAGAGGCTGGGGCTCTGTCCACCCGTGCCCGCTTCG TGCCCCGAGATTTCTCGGCCTGCAGGCTGTGGCTGCTGCCCGACATGTGCCTTGCCGATGGGTGCTGCCT GCGGTGTGGCCACTGCTCGCTGCGCCCAGGGACTCAGCTGCCGTGCGTTGCCAGGAGAGCCTCGTCCCCT GCATGCCCTCACCCGTGGTCAGGGAGCCTGTGTACCAGAACCTGCTGCACCCGCCACGAGCACCTTGTCC AGCTCCCAGCATGAAGAGGCAAAGGCTGCTGTGGTCTCCGCGGATGAGCTTTCTGAGAGCCCAGAGATGA CAGAGGAGCAGCTGCTGGACAGCTTCCACCTGATGGCCCCATCCCGCGAGGATCAGCCCATCCTGTGGAA CGCCATCAGCACCTATAGCAGCATGCGGGCCCGGGAGATCGCCGACCTCAAGAAATGGAAGGAGCCCTGC CAACGAGAACTCTATAAAGTGCTAGAGAGATTAGCTGCAGCCCAACAGAAAGCAGGAGATGAAATCTACA AATTTTATCTGCCAAACTGCAACAAGAATGGATTTTATCACAGCAAACAGTGTGAGACATCCCTGGATGG AGAAGCTGGACTCTGCTGGTGTGTCTACCCATGGAGTGGAAAGAAAATCCCTGGGTCTCTGGAGACTAGA GGGGACCCCAACTGCCACCAGTATTTTAATGTACATAACTGAAAGGAGTTCTTTTCTTTTTCCGTGTTCA CACAAAATATTTAAGTATATAGTATATTTATACTCTGGAGCACACCATTTTATATATGTGTATATGTATA TATCCAGGAACTAGTTTTTATACTCCACATACTGCTTGATACATGAAGCTGGTTTGTATTTATTCACTGT AAGTTTATTTTTTTCTACACAGTTCTTGTGCTATATTAATTTATATAACATGAAGCTCTTCTCATCTCTG TACATGTAAATACTATATCTCAGCTCTTGCAAAGTTCTGCTTTGAAAGGGCAGAGTGATGCTGCCTAGAA CAAGGCAGTCTGAGGCCGGAAGCTTTCAGTGGGTTAGGGGAGGGAGGTTGGCCCACACTCGGAGATTTCC TCATCGTCTCACACAGATCTGTGTCTGATGCACATTTGCTGGGAAAGTTCCGATGCTGATTGTGTAACCA AAGCTGAACGTGGAAAGCTGCGTCCCATACATTGTTAAATACACATCTGGAATAAATATTCTATCTGGAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4161889 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4161889 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003866 same experiment
	EMAGE:29826 same embryo
	EMAGE:32051 same embryo
	EMAGE:29830 same embryo
	EMAGE:32049 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source