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EMAGE:32096

Cacng8 ( MGI:1932376)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
cerebral cortex marginal layer
moderate moderate
homogeneousmoderate expression: see section 01 02 03 04 05 06 07 09 10 11 14 15 16 17 18 19 20 21 22
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5339
Entity Detected:Cacng8, ( MGI:1932376)
Sequence:sense strand is shown

>T5339
TACGGGCATCCGGCTTAGGCTCATGACCATCGCCATCAGCACTGACTACTGGCTCTACACAAGAGCTCTC
ATCTGCAACACCACCAACCTCACAGCAGGTGATGACGGACCACCCCATCGTGGGGGCAGTGGCTCCTCCG
AGAAGAAGGACCCTGGGGGCCTCACACATTCAGGCCTCTGGCGGATATGCTGCCTGGAAGGGTTGAAAAG
AGGTGTCTGCGTGAAGATCAACCACTTCCCGGAGGACACGGACTACGACCACGACAGCGCGGAGTACCTG
CTCCGAGTAGTCCGGGCTTCCAGCATCTTTCCTATCCTGAGCGCCATCCTGCTGCTGCTCGGGGGCGTGT
GCGTAGCTGCCTCCCGCGTCTACAAGTCCAAAAGGAACATCATCCTGGGCGCAGGGATCCTGTTCGTGGC
AGCAGGCTTGAGCAACATCATCGGGGTGATTGTGTACATATCGGCCAACGCCGGCGAGCCTGGCCCCAAG
AGGGACGAGGAGAAGAAAAACCACTACTCGTACGGCTATAGTGTCAACAAAAATAGCAG
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002189 same experiment
 EMAGE:29351 same embryo
 EMAGE:29770 same embryo
 EMAGE:29918 same embryo
 EMAGE:32239 same embryo
 EMAGE:29922 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS