EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:32239

Tsg101 tumor susceptibility gene 101 ( MGI:106581)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002131_01	euxassay_002131_02	euxassay_002131_03	euxassay_002131_04	euxassay_002131_05

euxassay_002131_06	euxassay_002131_07	euxassay_002131_08	euxassay_002131_09	euxassay_002131_10

euxassay_002131_11	euxassay_002131_12	euxassay_002131_13	euxassay_002131_14	euxassay_002131_15

euxassay_002131_16	euxassay_002131_17	euxassay_002131_18	euxassay_002131_19	euxassay_002131_20

euxassay_002131_21	euxassay_002131_22	euxassay_002131_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 16 17 18 19 moderate expression: see section 02 03

cervical ganglion

moderate

regional

moderate expression: see section 06 07 15

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 07 14

spinal cord

strong

homogeneous

strong expression: see section 05 06 07 08 09 10 11 12 13

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5506
Entity Detected:	Tsg101, tumor susceptibility gene 101 ( MGI:106581)
Sequence:	sense strand is shown >T5506 AACCACCCCTTCAACCACCTCACTGTCTGGCGGCCTGGGGCGGCCCCGTGGCGCCTACCCTCTGCCTGTG GGGACGGAGGAGCGCGCCATGGCGGTGTCCGAGAGTCAGCTGAAGAAGATGATGTCCAAGTACAAATATA GAGATCTAACCGTCCGTCAAACTGTCAATGTCATCGCTATGTACAAAGATCTCAAACCTGTATTGGATTC ATATGTTTTTAATGATGGCAGTTCCAGGGAGCTGGTGAACCTCACTGGTACAATCCCAGTGCGTTATCGA GGTAATATATATAATATTCCAATATGCCTGTGGCTGCTGGACACATACCCATATAACCCCCCTATCTGTT TTGTTAAGCCTACTAGTTCAATGACTATTAAAACAGGAAAGCATGTGGATGCAAATGGGAAAATCTACCT ACCTTATCTACATGACTGGAAACATCCACGGTCAGAGTTGCTGGAGCTTATTCAAATCATGATTGTGATA TTTGGAGAGGAGCCTCCAGTGTTCTCCCGGCCTACTGTTTCTGCATCCTACCCACCATACACAGCAACAG GGCCACCAAATACCTCCTACATGCCAGGCATGCCAAGTGGAATCTCTGCATATCCATCTGGATACCCTCC CAACCCCAGTGGTTATCCTGGCTGTCCTTACCCACCTGCTGGCCCATACCCTGCCACAACAAGCTCACAG TACCCTTCCCAGCCTCCTGTGACCACTGTTGGTCCCAGCAGAGATGGCACAATCAGTGAGGACACTATCC GTGCATCTCTCATCTCAGCAGTCAGTGACAAACTGAGATGGCGGATGAAGGAGGAAATGGATGGTGCCCA GGCAGAGCTTAATGCCTTGAAACGAACAGAGGAAGATCTGAAAAAAGGCCACCAGAAACTGGAAGAGATG GTCACCCGCTTAGATCAAGAAGTAGCTGAAGTTGATAAAAACATAGAACTTTTGAAAAAGAAGGATGAAG AACTAAGTTCTGCTCTGGAGAAAATGGAAAATCAATCTGAAAATAATGATATTGATGAAGTTATCATTCC CACAGCCCCACTGTATAAACAGATTCTAAATCTGTATGCAGAGGAAAATGCTATTGAAGACACTATCTTT TACCTTGGAGAAGCTTTGCGGCGGGGAGTCATAGACCTGGATGTGTTCCTGAAACACGTCCGCCTCCTGT CCCGTAAACAGTTCCAGCTAAGGGCACTAATGCAAAAGGCAAGGAAGACTGCGGGCCTTAGTGACCTCTA CTGACATGTGCTGTCAGCTGGAGACCGACCTCTCCGTAAAGCATTCTTTTCTTCTTCTTTTTCTCATCAG TAGAACCCACAATAAGTTATTGCAGTTTATCATTCAAGTGTTAAATATTTTGAATCAATAATATATTTTC TGTTTCCTTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6395694 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6395694 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002131 same experiment
	EMAGE:29351 same embryo
	EMAGE:29770 same embryo
	EMAGE:29918 same embryo
	EMAGE:32096 same embryo
	EMAGE:29922 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source