EMAGE mouse gene expression spatial database

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EMAGE:32167

Il6st ( MGI:96560)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006504_01	euxassay_006504_02	euxassay_006504_03	euxassay_006504_04	euxassay_006504_05

euxassay_006504_06	euxassay_006504_07	euxassay_006504_08	euxassay_006504_09	euxassay_006504_10

euxassay_006504_11	euxassay_006504_12	euxassay_006504_13	euxassay_006504_14	euxassay_006504_15

euxassay_006504_16	euxassay_006504_17	euxassay_006504_18	euxassay_006504_19	euxassay_006504_20

euxassay_006504_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9238
Entity Detected:	Il6st, ( MGI:96560)
Sequence:	sense strand is shown >T9238 GGCGGACGGAGGGTGGCGTGTGCGGTCGGGAGGCCAGAGCTTCGAGCCATCCGGGCTGCGGCTCCGCTGA GGGACCGGTGGTGTGGGCCGGGATCCGCGGGGCTGAGGGACGCTCAGGAGCGGCGAGGCTCGCGTCCGGA AGGAAGACCCGATAGAAGGAACATGACATCTAGAGGCAGCGAACTTGTTTCCGATTCATGCTTTATCATT TCTTAATTTCGTATGTTGGGAACATCCCTGCAAGATGTCAGCACCAAGGATTTGGCTAGCGCAAGCTTTG CTTTTTTTCCTCACCACTGAATCTATAGGTCAACTTTTGGAACCGTGTGGTTACATCTACCCTGAATTTC CAGTTGTCCAGCGCGGCTCGAACTTCACTGCCATTTGTGTGCTGAAGGAGGCGTGTCTGCAGCATTACTA CGTGAATGCCAGCTACATCGTGTGGAAGACCAACCATGCTGCTGTTCCCAGGGAGCAGGTCACTGTCATC AACAGAACCACGTCCAGTGTCACGTTCACAGACGTGGTCCTCCCGAGCGTGCAGCTCACCTGCAACATCC TGTCCTTTGGGCAGATCGAGCAGAATGTGTATGGAGTCACCATGCTTTCAGGCTTTCCTCCAGATAAACC TACAAATTTGACTTGCATTGTGAATGAGGGGAAGAATATGCTGTGCCAGTGGGACCCCGGAAGGGAGACT TACCTTGAAACAAACTACACTTTGAAATCAGAGTGGGCAACAGAGAAGTTTCCTGATTGCCAGTCAAAGC ATGGCACTTCATGTATGGTCAGCTACATGCCCACCTATTATGTCAACATTGAAGTCTGGGTGGAAGCAGA GAATGCCCTTGGGAAGGTCTCCTCAGAGTCTATCAATTTTGACCCCGTGGATAAAGTGAAACCCACCCCA CCATATAATTTATCAGTGACCAACTCAGAAGAATTATCCAGTATATTAAAGCTATCATGGGTCAGTTCAG GGCTGGGCGGTCTTTTAGATCTAAAGTCTGACATCCAATATAGGACCAAAGATGCCTCAACTTGGATCCA GGTCCCTCTTGAAGATACAATGTCTCCTCGAACTTCCTTCACTGTGCAGGACCTCAAGCCTTTTACAGAA TATGTGTTTAGGATCCGGTCCATTAAGGACAGTGGGAAGGGCTACTGGAGTGACTGGAGTGAGGAGGCTA GTGGGACCACATACGAAGACAGACCATCCAGACCACCAAGTTTCTGGTATAAGACAAATCCATCCCATGG GCAGGAATATAGATCTGTACGGCTCATATGGAAGGCACTGCCTCTTTCTGAAGCCAATGGGAAAATCTTG GATTATGAAGTGATTCTTACGCAGTCAAAGTCCGTCTCACAAACGTACACAGTCACTGGCACAGAGCTGA CCGTGAATCTCACCAATGACCGCTATGTCGCGTCTCTAGCAGCAAGAAACAAGGTGGGCAAATCAGCTGC AGCTGTCCTCACCATCCCCAGCCCCCACGTCACAGCTGCTTATTCTGTAGTGAATCTTAAAGCATTTCCA AAAGATAACCTGCTCTGGGTGGAATGGACACCTCCACCTAAACCCGTGAGCAAGTACATCTTAGAGTGGT GTGTGTTGTCAGAGAACGCACCCTGTGTTGAAGACTGGCAGCAGGAAGACGCTACCGTGAATCGGACCCA CTTGAGAGGACGCCTCCTGGAGAGCAAGTGCTATCAAATCACAGTAACTCCCGTATTCGCCACGGGGCCC GGAGGCTCTGAGTCCTTGAAGGCGTACCTCAAACAAGCCGCTCCTGCCAGAGGACCGACTGTTCGGACAA AGAAAGTGGGGAAAAATGAAGCTGTCTTAGCGTGGGACCAGATTCCTGTGGACGACCAGAATGGCTTCAT TAGAAACTACTCCATATCTTACAGAACCAGCGTGGGAAAGGAGATGGTTGTGCATGTGGATTCTTCTCAC ACGGAGTACACGCTGTCCTCTCTGAGTAGTGATACGTTGTACATGGTCCGAATGGCCGCGTACACAGATG AAGGTGGGAAAGATGGGCCGGAATTCACTTTTACAACACCAAAGTTCGCTCAAGGAGAAATAGAAGCCAT AGTCGTGCCTGTGTGCTTAGCCTTCCTCCTGACAACCCTGCTGGGCGTCTTGTTCTGCTTTAACAAACGA GATCTAATTAAAAAACACATCTGGCCTAATGTTCCTGATCCTTCCAAGAGTCATATTGCCCAGTGGTCAC CTCACACCCCCCCAAGGCACAATTTTAACTCCAAAGATCAAATGTACTCGGACGGCAATTTCACTGATGT AAGCGTTGTGGAAATAGAAGCAAACAACAAGAAGCCTTGTCCAGATGACCTGAAGTCCGTGGACCTGTTC AAGAAGGAGAAAGTGAGTACAGAAGGGCACAGCAGTGGCATCGGGGGCTCTTCATGCATGTCCTCCTCCA GGCCCAGCATCTCCAGCAACGAGGAGAATGAGTCTGCTCAGAGCACCGCCAGCACGGTGCAGTACTCCAC TGTGGTGCACAGCGGCTACAGGCACCAGGTCCCGTCCGTGCAAGTGTTCTCAAGGTCCGAGTCCACCCAG CCCCTGCTAGACTCGGAGGAGCGGCCAGAAGACCTGCAGCTGGTGGATAGTGTAGACGGTGGGGATGAGA TCTTGCCCAGGCAATCGTATTTCAAGCAGAACTGCAGTCAGCCTGAAGCCTGTCCAGAGATTTCACATTT TGAAAGGTCAAACCAGGTTTTGTCCGGCAATGAGGAGGATTTTGTCAGACTGAAGCAGCAGCAGGTTTCA GATCACATTTCTCAGCCCTATGGATCCGAGCAACGGAGGCTGTTTCAGGAAGGCTCTACAGCGGATGCTC TTGGCACGGGGGCTGATGGACAGATGGAGAGATTTGAATCTGTTGGAATGGAGACCACAATTGATGAAGA AATTCCCAAAAGTTACTTGCCACAGACTGTAAGACAAGGTGGCTACATGCCGCAGTGAAGGACTGGCTCC TGAACTTCAGCAGGAACTGCAAAATAAAGCTAAAGACGAGTGGCTTCAGATGAGAAACAGTCCTCACTCC CTGAAGATAGGCATTGCCTCTAAGGACAAAGTCACACCTGGGCCGTCTCCATTCCAGAGTAGCTGGAATT CTCCTTCAAGCACTACATGAACTGACTTTATCCTCTATAGCTGGAGGACTCTGTGCTCTTTCAGAATGTT TGCACTGCCGAAAACAAAGTGTGTCTGAGTTTATAAAGCTGTTGGTTTGCTACATAGAAAACGGGGGATT TAAATGAGCCACAATGTCATGGCATCGTTCAGCTGGGCTGGTTTTGATGAGACTGACCATCCAGATGCCA GCGAGAAGGCCAGCAGCATCATTTAAAAGTGCCCTGTGGCACAGTGTCCAGATTATGTCATCTAGAAGAC GAAAGAATGGAGGGGTGGCTCGTTTCTGGGTCTGTCAGAGAAGCATGGCCTGCTCCTTGCTGTTGGTATC GATGCCGGTTACTACTGAGGAAGAGGATAAACTTTAAAGAGAAGCAACATAGAAGCACCCGCTTTCTTAT AGTCACCTACAGCCTCCCAAGAAACTGCTTATTATTGAAAATGTCTTTCCGAGTCAATTTCATCCACCCT ATAGTGGACCAGGCTGGCCTCAAACTCAGAGATCTGTCTGCCTCTGCCTCTGCCTCTGCCTCCAGAGTAC CGGGATGAAAGGCAGTGCACCACCACTGCTCCACTTACGAAGATTGCTGTTTTTAAAGACTTAAGTTGTT GACATTTTTCTGAACACACATAACCACACCAGGGACAGATGATTCTAGAGACTGGTTGAGGAAGCACTTT ATCCAGAATGTCTTCAACTTTAAATTACTTTAATTGACTCCACCTTGTTTTGATTTTCAAGAATAGCAGG GTGTTTGTTGAAGGGTGGCATTTCCCAGAGCCTCATGCAGTCCCAGAACACACATCCCAACCATGTCAGC GACCACCACAATGACAGAAGTCCTCCTACAGAGCTGAGGAAGCTGGAAGGGTCCTGCTAGTTTGCAGGGT GGGCTTTGAACCCACAGCCCTGTGCATGCTAGACATTCACTCTACCGCTGAGCTGCTCCTCCGCCCGGCT CATTATGTCATCTAGGCTCTCGTGATCCTCCTGGCCCAGCTTCCTGACTGTCTGGGACTGTGGATGTGTG CTGTCACACACCCAACCAGGTCTTTTTAATATCTGCCATTTATGAGGTAGAGTTTTTACACGACTTAGGA CTATCTTAAACCTATTCCTTCTAGTTGCTTTATTAAGGAATAGCCTAGGCATCCAGTGTTTAGCACTAAA GCCATTAATGTATTTGTGCCTAACTTCCCAGTGTGACATGGAGGTGGGGGCTAAAGGTAGACACTGGGCA GACAGGAATTGGCTGAGCGCCGTTAGAGGCTGATCACTAAGTGTGCTCACAGGAAAAGGGTTGAAGTTTC AGAGCATGGGCACCCAGATCATTTATAGCGGTTACCACACAGTGCCTAGGAGGAGTTGATATGCACTTAG TTGGATGCAGTTTCACCTTTTACTCTCAGAAGGTTGTTTATATTCTACTTGCTGATGTGTTATCATTTAT ATTTTTATACTGGGTAGTCCTTTTCTGTGTTCTTTCACATGTATAATCCTCGTGAGACTGTACATGCTTT GCTCTGGTATATTCAGTTGCCTCCTTGGCAAAACCAGCCTGGAATTGCCAAAATGCCGGAAGTGAAGGGA GATGGTGAAAGTCGGGCACAGGAAGGCCAGGTCAGGGGCAGAGAGACCAGAATTCACACTACAAGGCTGG CACCATTGCCATTCAAGTTGGACGTCAAAGCCTGATCCCAGTTAGGACTGAGTTTTCTATGTATATATCT GCCAGGAAGTTAACACTTGCTGAATTTCTCATCTTCTCACAGTAAGCAGTCTAGTTATGGGACTCTATCC ATGTTCAGCTGTGACTTCCCTCATATCAGACAGTGGTGCTGTGAACGTTGCAGTGTGAAGTCTGTACTGG CTAGGGATGGTAGCCCAGCGTGAGTTCAGGCATGAACATAGAGGCCACTGAAGGAAGAGCCTCCTGGAAG CAAGTTGGCACGTTAAATCCCAGACAGAAAGTGTCCCAGGGAGCCAGGGCCTCCGCCAGCAGGGAGGGGG TGTGCTCACTGCAGGAACGTTCATTTAGAAGATGTACCATGGAGCACCTCCCTTTGCTCTAAGAGAAAAC CCATCTTAAAGTTCATCTTGAATACAGACTTCGCTCGAGCATGTTTTAGAATAATCTGGATGCTGTACCT TGTACCTTAGGTAGCAGAGAAAGTAATCTGGCGTCTTTCTGTAAAACCGTCTAATAAAGCTAGTTCTGCC CGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6834623 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834623 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006504 same experiment
	EMAGE:29882 same embryo
	EMAGE:29871 same embryo
	EMAGE:29226 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source