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EMAGE:32240

Cblc ( MGI:1931457)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240
euxassay_004794_01 euxassay_004794_02 euxassay_004794_03 euxassay_004794_04 euxassay_004794_05
EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240
euxassay_004794_06 euxassay_004794_07 euxassay_004794_08 euxassay_004794_09 euxassay_004794_10
EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240
euxassay_004794_11 euxassay_004794_12 euxassay_004794_13 euxassay_004794_14 euxassay_004794_15
EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240
euxassay_004794_16 euxassay_004794_17 euxassay_004794_18 euxassay_004794_19 euxassay_004794_20
EMAGE:32240 EMAGE:32240 EMAGE:32240 EMAGE:32240
euxassay_004794_21 euxassay_004794_22 euxassay_004794_23 euxassay_004794_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
oral epithelium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
stomach
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 04 05 06 07 08 10
metanephros
weak weak
regionalweak expression: see section 05 06 07 08 14 15 16 17
rectum
moderate moderate
regionalmoderate expression: see section 13 14
urethra of male
moderate moderate
regionalmoderate expression: see section 13 14 15
midgut
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 14 15 17 18 weak expression: see section 16
thyroid gland
weak weak
regionalweak expression: see section 11 12
conjunctival sac
weak weak
regionalweak expression: see section 01 02 03 04 05 22 23 24
bladder
moderate moderate
regionalmoderate expression: see section 13 14
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 21 22 23 weak expression: see section 01 02 03 04 05 06 20
thymus primordium
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 09 10 11
naso-lacrimal duct
weak weak
regionalweak expression: see section 04 05 06 07 08 09 10 11 12 18 19 20 21 22 23
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 15 16 17 18 19
naris
moderate moderate
regionalmoderate expression: see section 13 17 18
pharynx epithelium
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 08 09 10 11 12
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10
lung
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5510
Entity Detected:Cblc, ( MGI:1931457)
Sequence:sense strand is shown

>T5510
CCCATGGCTGCGGCGGCAGCCCCGCGAGGGTGGCAGCGGGGCGAGCCACGCGCGCTTAGCCGGGCGGTGA
AGTTGCTCCAGCGCCTAGAAGAGCAATGCAGGGATCCCAGGATGGTCACGGGGCCCCCGTCCCTGCGGGA
CCTGCTGCCCCGCACCGCGCAGCTACTTGGAGAGGTGGCAAAGGCCCGGCGCGAGGCCAGGGAAGACCCC
GAGGGTCCCGGTGGCGCCGATGACTTTCTGGCCATCTACCTGGCCAATCTGGAGGTCAAAGGCAGGCAGG
TGGCGGAGCTCCTGCCACCCCGAGGCAAAAAGGACGTGAACCAGGATGTTTTCCGGGAGGGCTCCAGATT
CAGGCGACAACTGGCCAAGCTGGCCCTCATCTTCAGTCACATGCACGCGGAGTTGAGCGCACTCTTCCCT
GCTGGGAAGTACTGTGGGCACCTGTACCAGCTCACCAAGGGCTCTGCCCACATCTTCTGGAGGCAGAATT
GTGGAGTTCGGTGCGTGCTTCCCTGGGCTGAGTTCCAGTCCCTCCTGTGTGCCTGCCACCCTGTGGAACC
AGGCCCCACCATGCAGGCCTTGCGGTCCACCTTGGACCTCACCTGTAACGGCCACGTGTCTGTCTTTGAG
TTTGACGTCTTCACCAGGCTCTTTCAGCCGTGGCCCACTTTACTGAGGAATTGGCAACTCCTGGCTGTCA
ACCATCCTGGCTACATGGCCTTCCTCACCTACGATGAGGTCCAAACACGCCTGCAGGCCTACAGGGACAA
ACCAGGCAGCTATATCTTCCGGCCAAGCTGTACCCGCCTGGGGCAGTGGGCCATTGGATACGTGAGCTCC
GATGGAAGCATCCTGCAAACCATTCCTCTCAACAAACCTCTGCTGCAGGTGCTCCTGAAGGGACAAAAGG
ACGGCATCTTCCTCTTCCCTGATGGAAAGAAACACAACCCAGACCTGACTGAGCTCTGCCGGGTAGAACC
CTACCAACGCATCCAAGTGTCAGAGGAACAACTGCTGCTCTACCAAGCCATGAACTCCACGTTCCAGCTG
TGCAAGATCTGCGCAGAGAGGGACAAGGACGTGAGGATCGAGCCCTGTGGGCATCTTCTCTGCAGCTGCT
GCCTGGCTGCCTGGCAGGACTCAGACAGCCAGACCTGTCCCTTCTGCCGTTGTGAGATCAAAGGTCGAGA
GGCCGTGAGTATCTGTCAGGCACAGGAGAGGCCAACGGAGGTCAGGACTGCTGCAGATGACTCAAGAGAC
AACTGTCACCAGGAGGCTGCTGAGCAGAAACTGGGGCCGGTGATTCCCTCTGCTCCCTCACTACTCCCTG
AAGATCAGTTCCCTCAGGGACCCCAAGACAAAGGCTGGCTGACACTGGCACCACTCGCCCTGCCCAGGCT
CCGACCTCCACTTCCTCTCCCCAAAATGGCCTCGGTTCTGTGGGAAGTCACCTCCAGGCCCCGGGCCAGG
GAGGAGGCCACAGAAAGCTCCTGAGAGGGAAAACACCCCTCCCGCAGACCCCACAGGACCCCGAGTTGTG
CCCAAAACCTAGAGGCTAGGGCACCTGGACTGGAGGAAGGGAGCCTTGGGGCTGGAAGGAGTGACAAAGC
AGAAATAAATTGAATCCTGATCTTTCAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6395806 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6395806 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004794 same experiment
 EMAGE:32098 same embryo
 EMAGE:32243 same embryo
 EMAGE:29866 same embryo
 EMAGE:29635 same embryo
 EMAGE:30114 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS