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EMAGE:32243

Casp6 ( MGI:1312921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243
euxassay_004793_01 euxassay_004793_02 euxassay_004793_03 euxassay_004793_04 euxassay_004793_05
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euxassay_004793_06 euxassay_004793_07 euxassay_004793_08 euxassay_004793_09 euxassay_004793_10
EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243
euxassay_004793_11 euxassay_004793_12 euxassay_004793_13 euxassay_004793_14 euxassay_004793_15
EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243 EMAGE:32243
euxassay_004793_16 euxassay_004793_17 euxassay_004793_18 euxassay_004793_19 euxassay_004793_20
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euxassay_004793_21 euxassay_004793_22 euxassay_004793_23 euxassay_004793_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
metanephros
moderate moderate
regionalmoderate expression: see section 17 weak expression: see section 06 07 08 15 16
testis
weak weak
spottedweak expression: see section 04 05 06 07 19
bladder
moderate moderate
regionalmoderate expression: see section 12 13 14
pancreas
weak weak
regionalweak expression: see section 07 08 09 10 11
pharyngo-tympanic tube
moderate moderate
regionalmoderate expression: see section 01 02 21 22 23 24
thymus primordium
moderate moderate
regionalmoderate expression: see section 09 10 11 12
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 13 17
vibrissa
weak weak
regionalweak expression: see section 06 07 08 09 22 23
telencephalon ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16 17 18 19 20 21
midbrain ventricular layer
weak weak
regionalweak expression: see section 08 09 10 11 12 13
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 11 12 13 15 17 18 19
naris
moderate moderate
regionalmoderate expression: see section 17
inner ear
moderate moderate
regionalmoderate expression: see section 21 weak expression: see section 06 07 08 14 15 16 17 18
medulla oblongata basal plate ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16 17 18
cerebellum intraventricular portion
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16 17 18
stomach
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08
pons ventricular layer
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16 17 18
urethra of male
moderate moderate
regionalmoderate expression: see section 14 15
pons mantle layer
weak weak
regionalweak expression: see section 13
midgut
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18
adenohypophysis
weak weak
regionalweak expression: see section 09 10 11 12 13 14
medulla oblongata basal plate mantle layer
weak weak
regionalweak expression: see section 14
heart
moderate moderate
regionalmoderate expression: see section 09 11 12
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 12 15 weak expression: see section 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 12 weak expression: see section 16 17
lung
moderate moderate
regionalmoderate expression: see section 13 14 15 17 18 weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 16 19 20 21
diencephalon lateral wall ventricular layer
weak weak
regionalweak expression: see section 11 12 13 14
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6068
Entity Detected:Casp6, ( MGI:1312921)
Sequence:sense strand is shown

>T6068
CGGAGGCCTCAGGGCTAGGACACCGGTGGGAGCTCGCGGGCAGGTGAAAGTAAAACATGACAGAAACCGA
TGGCTTCTACAAAAGTAGGGAAGTGTTCGATCCAGCCGAGCAGTACAAGATGGACCACAAGAGGAGAGGA
GTTGCCCTCATCTTCAATCACGAGAGGTTCTTTTGGCACCTGACCCTCCCAGAGAGGCGGGGCACCAACG
CAGACAGAGACAACCTGACTCGCAGGTTTTCAGATCTAGGATTTGAAGTGAAATGCTTTAACGACCTCAG
AGCAGAAGAACTCCTGCTCAAAATTCACGAGGTGTCGACTTCAAGCCACATAGATGCCGATTGCTTCATC
TGTGTCTTCCTGAGCCACGGGGAAGGCAACCACGTTTACGCATACGACGCCAAAATTGAAATTCAGACGT
TGACTGGCTTGTTCAAAGGAGACAAATGTCAGAGCCTGGTTGGAAAACCCAAGATATTTATCATCCAGGC
CTGTCGGGGCAGCCAGCATGACGTACCTGTGGTTCCCCTGGACGTGGTGGATCACCAGACAGACAAGCTG
GACAACGTGACCCAGGTGGATGCTGCGTCTGTGTACACGCTGCCGGCAGGGGCAGACTTCCTCATGTGCT
ACTCTGTCGCAGAAGGGTATTACTCTCACCGAGAAACTGTGAATGGCTCCTGGTACATTCAGGATTTGTG
TGAGATGCTGGCGAGGTACGGCAGTTCCCTGGAGTTCACGGAGCTGCTCACGCTGGTGAACAGAAAGGTC
TCTCAGCGCCGCGTGGACTTCTGCAAAGACCCGGATGCAATCGGCAAGAAGCAGGTGCCCTGCTTCGCCT
CAATGCTGACCAAAAAGCTGCATTTCTGTCCCAAACCTAGCAAGTAGGGCCATCTGTCTTGCTACATATA
TTTCACACACATTTCCCTTTTTATACAAAGGCCAGCTGGTGGAAGACTTTCACCCATCAATGTAAACTGC
TTCAATTTTGTAGATGCCATAGAAAAGTAATGTTTTATATTTGTTACTTTTCAATCATGTTGGACGTGGT
GGAAGGGCTAAAAATAATCCTCAGGAAATTACTATGCATCAATGTCTACTACATTTATTACATTTTTAGT
AAACTGTAAGATTACTCTAAATACCAACTACTTTGCCTCCCCTTTTGTGACACTGTTTATATGAGTTTAC
TGTATAATGTTTGTAAAGACTTTTGAAACTCTAGACTGAGATTGTTTTCGCGTAATAAAATGTCTAAGAA
CTCATAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3491126 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3491126 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_004793 same experiment
 EMAGE:32240 same embryo
 EMAGE:32098 same embryo
 EMAGE:29866 same embryo
 EMAGE:29635 same embryo
 EMAGE:30114 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS