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EMAGE:6143

Wnt5a wingless-related MMTV integration site 5A ( MGI:98958)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6143 EMAGE:6143 EMAGE:6143 EMAGE:6143 EMAGE:6143
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:6143Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
6143_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6143_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt5a probeB
Entity Detected:Wnt5a, wingless-related MMTV integration site 5A ( MGI:98958)
Sequence:sense strand is shown

>Wnt5a probeB
GAATTCCTCGGCCGCCTTCGCTCGGGTGGCGACTTCCTCTCCGTGCCCCCTCCCCCTCGCCATGAAGAAG
CCCATTGGAATATTAAGCCCGGGAGTGGCTTTGGGGACCGCTGGAGGTGCCATGTCTTCCAAGTTCTTCC
TAATGGCTTTGGCCACGTTTTTCTCCTTCGCCCAGGTTGTTATAGAAGCTAATTCTTGGTGGTCTCTAGG
TATGAATAACCCTGTTCAGATGTCAGAAGTATATATCATAGGTGCACAGCCTCTCTGCAGCCAACTGGCA
GGACTTTCTCAAGGACAGAAGAAACTCTGCCACTTGTATCAGGACCACATGCAGTACATTGGAGAAGGTG
CGAAGACAGGCATCAAGGAATGCCAGTACCAGTTCCGGCATCGGAGATGGAACTGCAGCACAGTGGACAA
TACTTCTGTCTTTGGCAGGGTGATGCAAATAGGCAGCCGAGAGACGGCCTTCACGTACGCGGTGAGCGCA
GCTGGGGTGGTGAACGCCATGAGCCGAGCATGCCGGGAGGGCGAGCTGTCTACCTGTGGCTGCAGCCGCG
CTGCGCGCCCCAAGGACCTGCCTCGGGACTGGTTGTGGGGCGGCTGCGGAGACAACATCGACTATGGCTA
CCGCTTCGCCAAGGAGTTCGTGGACGCTAGAGAAAGGGAACGAATCCACGCTAAGGGTTCCTATGAGAGC
GCACGCATCCTCATGAACTTACACAACAATGAAGCAGGCCGTAGGACAGTATACAACCTGGCAGATGTAG
CCTGTAAGTGTCATGGAGTGTCTGGCTCCTGTAGCCTCAAGACGTGCTGGCTGCAGCTGGCGGACTTCCG
GAAGGTGGGCGATGCCCTCAAGGAGAAGTATGATAGCGCGGCGGCCATGAGGCTCAACAGCCGGGGCAAG
CTGGTGCAGGTCAACAGCCGCTTCAACTCCCCGACCACGCAGGACCTGGTCTACATCGACCCCAGTCCGG
ACTACTGTGTGCGCAACGAGAGCACTGGCTCGCTGGGCACGCAGGGACGCCTGTGCAACAAGACCTCAGA
GGGGATGGACGGCTGCGAGCTCATGTGCTGTGGGCGTGGCTATGACCAGTTTAAGACAGTGCAGACCGAA
CGCTGTCATTGCAAGTTTCACTGGTGCTGCTATGTCAAATGCAAGAAGTGCACGGAGATTGTGGATCAGT
TCGTGTGCAAATAGTGGTGTGCCTGCCCTTCACCCAGTCCCACTCCCAGGACCCACTTATTTATAGAAAG
TACAGTGCTTCTGGTTCTTTTTATTTCTCCCCCAAGAATTGCAGCTGGAACCATGTGTTTTGTTTTGTTT
TATTTTGTTTTTTCTTTTCTGTTACCATCTAAGAACTCTGTGGTTTATTATTAATATTATAATTAATATT
TGGCAATAGTGGGGGAAACTAAGAAAAATATTTATTTTGAGGATCTTTGCAAAGTTAGTACAAAATTTCT
TTCTTCTGATGCTACAGGATAAAGGGGAAAAACTATGTATTCGAACTTAGCTGTGCAGTTGGGGGTTCAC
ATCTAGAAGGTGTAGGAGCCATTTTCTTCTCAAACAGAGAGTCCTTTGAGATGGGTGGTATCCAGGTGAA
GGAGGAGGTACAGACCCATGAATAACAGTTCCTGTGACCAAAATGAATTGCAGGTGCTCTGGTACAAAAG
ATCTTAAATATAGATATATTAAATATACATATATGCCAAAAATACAGAATATGAGACACTCCCTAACCCA
GAGGTTACCAGCCTGGTTTTGTGGGTTTTTTGTTTTGTTTTGTTTTTTCTTTTTTTGGGTTTTGTTTGTT
TGTTTGTTTGTTTGTATTTTTGGTGTGTGTGTGTGTGTATTTCTAGAATGATCTTTTAGAAGGTACAAGC
AAGAATCTCATATCTTCAGAAGCAGGCATATCATGTATGTTACTGTGTCCCACCTACAGATACTCCATTC
ATGAATGGGCCTTTTTCTAACAGTTCATGAATATTGGGGAGCCGGTGGGCTGGGGGAGGGAGGTCCCCAG
AAATTAGAAAACTTGAAGTTTCCTACATTGAGGCCATAATCTTGTGTTAGCCCAGCTGATTCTTAATACC
AGACTTTTAGATCCATAAAGGAATTTTTGACTAAAAAAA
nt 576 - nt 2714 of NM_009524.2
Notes:The template used to generate the Wnt5a probe used in this study was obtained from A. McMahon. Probe was transcribed from the entire insert. The insert sequence of the clone has been verified by Summerhurst and Murphy (it spans nt 576-2714 of the Wnt5a cDNA RefSeq NM_009524.2).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE