EMAGE mouse gene expression spatial database

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EMAGE:6718

St7 suppression of tumorigenicity 7 ( MGI:1927450)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002336. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002336_01	euxassay_002336_02	euxassay_002336_03	euxassay_002336_04

euxassay_002336_05	euxassay_002336_06	euxassay_002336_07	euxassay_002336_08	euxassay_002336_09

euxassay_002336_10	euxassay_002336_11	euxassay_002336_12	euxassay_002336_13	euxassay_002336_14

euxassay_002336_15	euxassay_002336_16	euxassay_002336_17	euxassay_002336_18	euxassay_002336_19

euxassay_002336_20	euxassay_002336_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6718_wholemount_strong.wlz

6718_wholemount_moderate.wlz

6718_wholemount_weak.wlz

6718_wholemount_possible.wlz

6718_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6718_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

spinal cord

weak

homogeneous

weak expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3938
Entity Detected:	St7, suppression of tumorigenicity 7 ( MGI:1927450)
Sequence:	sense strand is shown >T3938 GGAGAGTGCAGGGACCGGGCCCACGTGACCGCTCCACGAAGGCCACCGGCGGGGGCTCGGAGCGCAGGCC GCGCGCTTTCCCGCCGCATCCGGGCGCCGGGCGAGCTCCGGGGACGCCGGGCCCTGGCGGCGGCGGCGGC GGCTCGAGCCGTGCCTCGGGCGGGTGAATCATCCCGGCCGACAGCGAGCGCCGCGGCGGCCCGTAGCAGC GCCGGAAGATGGCCGAGGCGGGCGCGGGCTTCCTGGAGCAGCTCAAGTCCTGCATCGTGTGGTCGTGGAC CTATCTGTGGACCGTGTGGTTCTTCCTCGTGCTCTTCCTGGTCTACATCCTGAGGGTGCCCCTGAGGATC AACGACAACCTGAGCACAGTGAGCATGTTTTTGAACACGTTAACCCCAAAGTTCTACGTGGCCCTGACAG GCACTTCCTCGCTAATATCGGGACTTATTTTGATATTTGAATGGTGGTATTTTCGCAAGTATGGAACATC TTTCATTGAACAAGTCTCCGTAAGCCACTTGCGCCCCCTTCTGGGAGGGGTTGACAACAACTCTTCCAAT AATTCTAATTCCAGTAATGGAGACTCAGATTCCAACAGACAAAGTGTCTCAGAATGCAAAGTATGGCGAA ATCCACTGAATCTGTTTAGGGGTGCGGAATATAATCGGTACACTTGGGTCACAGGACGAGAGCCTCTGAC CTACTATGACATGAACCTCTCTGCCCAGGACCACCAGACATTCTTTACTTGTGATTCAGATCATCTTCGC CCTGCAGATGCAATAATGCAAAAAGCTTGGAGAGAAAGGAACCCTCAAGCCAGGATTTCTGCAGCTCATG AAGCCTTGGAGATAAACGAAATTAGGTCCAGAGTTGAAGTTCCCCTAATTGCTTCCTCTACCATCTGGGA GATAAAATTGTTACCAAAGTGTGCAACTGCGTACATTCTCTTGGCGGAAGAAGAAGCGACAACTATTGCT GAAGCAGAAAAACTCTTTAAGCAGGCCCTGAAGGCTGGAGATGGTTGTTACCGGCGCTCTCAGCAGCTAC AACATCATGGGTCCCAGTATGAAGCTCAACACAGACGAGACACCAATGTCTTAGTCTATATCAAAAGAAG GCTGGCGATGTGTGCCCGGAGACTTGGAAGGACCAGAGAAGCAGTGAAGATGATGAGAGATTTAATGAAG GAGTTTCCCCTCCTAAGCATGTTCAATATCCATGAGAACCTTCTAGAAGCTCTTCTGGAACTCCAAGCTT ATGCTGATGTTCAGGCAGTCTTAGCAAAGTATGATGATATAAGCTTACCAAAGTCGGCGACAATATGCTA CACAGCCGCCCTGCTCAAAGCAAGAGCTGTCTCTGACAAATTCTCTCCAGAGGCTGCGTCTCGGAGGGGG CTGAGCACAGCAGAGATGAATGCAGTAGAAGCCATCCACAGAGCTGTGGAATTTAATCCACACGTGCCAA AATATCTACTAGAAATGAAAAGCTTAATCCTCCCACCAGAACACATCCTGAAGAGAGGAGACAGTGAAGC GATAGCATATGCATTCTTTCATCTCGCACACTGGAAGAGGGTGGAAGGGGCTTTGAATCTCTTGCATTCT TTCCGGATGATCCCGTATCCCCTGGAGAAGGGACACCTATTTTATCCATACCCAATCTGTACAGAAACAG CTGACCGGGAGCTGCTTCCCTCTTTCCATGAAGTCTCAGTTTACCCAAAGAAGGAACTTCCCTTCTTCAT CCTCTTCACTGCTGGACTGTGCTCCTTCACAGCCATGCTGGCCCTCCTGACACATCAGTTTCCGGAACTT ATGGGAGTCTTCGCAAAAGCTTTCCTCAGCACTTTGTTTGCCCCCTTGAACTTTGTTATGGAGAAAGTGG AAAGCATCCTCCCCTCCAGTTTGTGGCATCAGCTGACACGGATCTGAGGGAGCCTCCCATCCTTCACTTT CCACACCATCCACCATCTCTTCTGTGCCAACCTCTCATGGACCGCAAGAAAGCATGACTTTGAAAAAGGG AAACCATTCTGAGATTTTAAACTGCTCATGGACTATCAGTTCCTTATTAAAGGCTGGTTTTGTTGTACAA AATTTATTGATGTTCAGTTCTATTTTATTTTGCCTTCAAAACTGACTGATCAAAAAGTAAAAAAAAATAA ATAAATAAACTTTTGTGTATTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3660309 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3660309 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6715 same embryo
	EMAGE:6713 same embryo
	EMAGE:6714 same embryo
	EMAGE:6716 same embryo
	EMAGE:6717 same embryo
	EurExpress:euxassay_002336 same experiment
	MGI:4828468 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source