EMAGE mouse gene expression spatial database

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EMAGE:6943

Bfar bifunctional apoptosis regulator ( MGI:1914368)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004546. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004546_01	euxassay_004546_02	euxassay_004546_03	euxassay_004546_04

euxassay_004546_05	euxassay_004546_06	euxassay_004546_07	euxassay_004546_08	euxassay_004546_09

euxassay_004546_10	euxassay_004546_11	euxassay_004546_12	euxassay_004546_13	euxassay_004546_14

euxassay_004546_15	euxassay_004546_16	euxassay_004546_17	euxassay_004546_18	euxassay_004546_19

euxassay_004546_20	euxassay_004546_21	euxassay_004546_22	euxassay_004546_23	euxassay_004546_24

euxassay_004546_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6943_wholemount_strong.wlz

6943_wholemount_moderate.wlz

6943_wholemount_weak.wlz

6943_wholemount_possible.wlz

6943_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6943_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7430
Entity Detected:	Bfar, bifunctional apoptosis regulator ( MGI:1914368)
Sequence:	sense strand is shown >T7430 TCTACTTCCGCGGGGTCCTGGCAGGTGGCGGTGACAGAGTGGGTACCTGGACTAGTGGCGAGACTTTATG ATGCATTGCAGCAGTGTCCTGAGTCTGAAAGTGTCTATGTGTATGGATCCCAGAATTTGCTGAGAGATGG AGGAGCCTCAGAAAAATGACCTGAGCATGAGAGAGCAGGAGGAAGAGCATCCTGTCAGAAGCAGCGGCCC TCAGATTTCTGTGAGCGAGTTCTCCTGCCACTGCTGCTACGACACCCTGGTTAACCCCACCACCTTGAAC TGTGGCCACAGCTTCTGCCGGCACTGCCTGGCTTTATGGTGGATGTCTTCAAAGAAGACAGAGTGTCCAG AATGCAGAGAAAAATGGGAAGGTTTTCCTAAAGTCAACATTCTCCTCAGGGATGCCATTGAAAAGTTATT TCCTGATGCCATTAGAATGCGAGTTGAAGACATTCAGCAGAATAATGATGTAGTCCAAAGTCTTGCAGCC TTTCAGAAATATGGGAATGATCAGAATCCCTTAGCTCCCAGCACAGGGCGAGTAAATCCTCAGAGAGGAG GGGGATTCTTCTCCGGTGTTCTCACAGCTTTAACTGGTGTGGCAGTCATCCTGCTTGTGTATCATTGGCG CAGCAGAGAATCTGAACATGGCCTCCTGGTGCACAAGGCTGTAGATAAGTGGACGATGGAAGAAGTTGTC CTCTGGTTAGAACAGCTAGGACCTTGGGCCTCCCTGTACAGAGACAGGTTCTTATCTGAAAGAGTAAATG GAAGGTTGCTTTTAACTTTGACAGAAGAAGAGTTTTCCAGGGCACCTTATACCATAGAGAACAGTAGCCA CAGAAGGGTCATCCTCACGGAGCTGGAGCGTGTGAGAGCCCTGGGAGTGAAGCCACCGCAGAATCTCTGG GAGTACAAGGCTGTCAACCCGGGTAGATCCCTATTCTTGCTCTACGCTCTCAAGAGCTCACCCAGACTTG GCTTGCTATACCTGTACCTGTTTGACTACACAGACTGCTTCCTACCCTTCATCCACACCATCTGCCCTCT GCAGGAAAACAGCTCTGGCGAGGACATTTTCACCAAGCTTCTGGACCTGAGGGAGCCCACGTGGAAGCAG TGGAGAGAATTCCTCGTCAAGTACTCCTTTCTTCCGTACCAGCTGATCGCGGAGTTTGCCTGGGACTGGC TGGAGGTCCACTACTGGACATCTCGCTTCCTGATCGTCAATGCCGTGCTGCTCTCTGTTCTGGAGTTGTT CTCTTTTTGGAGGATCTGGTCGAGAAGTGAGCTGAAGACAGTGCCCCAGAGGATGTGGAGCCACTTCTGG AAAGTATCGACACAGGGGCTTTTCATGGCCATGTTCTGGCCGCTTATTCCTCAGTTTGTCTGCAACTGTT TATTTTACTGGGCTCTGTACTTCAACCCAATTATTAACATTGATCTGGTGGTCAAGGAAGTCCGACGGCT GGAAACCCAAGTGTTATGATGGGCAGCAAGGCCCGAGCGAGCCCGTGAATCTACCTGACAACCAAGCTGT GACATTCAAAGAGACAAGGGACTCGTTTCTACCCCTGGTAATGCGAGGTGCTGCTTCTGTGTGTCAAGGC TTCAACCAGGTCCCTCTTCCTCTGACTCTGGCACCATGCCAGCTGACTGCTACCCAGGTCAGATTGGACT CATGGTCAATACCCAGCCAGTCTTTTTACAGGGACTTGAGAGGCTCAGGCTACTATGACCAATAGAAGTG AAAGTCTTGGTCATAATTTGGGGTGACATGCTGTCAAAAGGCTAATATAGACTGGGTGTGGTAGTGCACA CCTTGAATCTGAGCACTAGGAAAACAGGCAGGTGGATTTTTGAGTTCAAGACCAGAGTGGTCTACAGAGT GAGTTCTACAACAGCCAGGGCTACATAGAGGAACCATGTCTCTAAAAAAAAAAAAAAAGAAAGAAAGAAA AAGAAAAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4196466 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4196466 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6945 same embryo
	EMAGE:6942 same embryo
	EMAGE:6944 same embryo
	EurExpress:euxassay_004546 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source