EMAGE mouse gene expression spatial database

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EMAGE:6951

Arhgdib Rho, GDP dissociation inhibitor (GDI) beta ( MGI:101940)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002360. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002360_01	euxassay_002360_02	euxassay_002360_03	euxassay_002360_04

euxassay_002360_05	euxassay_002360_06	euxassay_002360_07	euxassay_002360_08	euxassay_002360_09

euxassay_002360_10	euxassay_002360_11	euxassay_002360_12	euxassay_002360_13	euxassay_002360_14

euxassay_002360_15	euxassay_002360_16	euxassay_002360_17	euxassay_002360_18	euxassay_002360_19

euxassay_002360_20	euxassay_002360_21	euxassay_002360_22	euxassay_002360_23	euxassay_002360_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6951_wholemount_strong.wlz

6951_wholemount_moderate.wlz

6951_wholemount_weak.wlz

6951_wholemount_possible.wlz

6951_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6951_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 18 19 20 21 22

spinal cord

weak

homogeneous

weak expression: see section 09 10 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1962
Entity Detected:	Arhgdib, Rho, GDP dissociation inhibitor (GDI) beta ( MGI:101940)
Sequence:	sense strand is shown >T1962 NTCCCTGCAAATGAGACTTAAAACCCTGGCTTCTCCCACCTTGAGTCCTGAAGGTCAAGAAATGGGCAAG CTCCGGCTGACGCCTCCTTGGCCTTCTGTTCAGAAGTTTATTCTTGCCACTGGGTTCTTCCGGTTCGATG AACCCCGTTGTCTTCTCTGCCAAGGGGAGCTCTCCTTTTGTGGGAGACACTGTAAACAACACCAAAGGAA AAGAATAAAATCGTTGTGTGTGTGACTGAAAAAAAAANAAAA
Notes:	The probe template was PCR amplified from IMAGE:641008 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:641008 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6952 same embryo
	EMAGE:6950 same embryo
	EMAGE:6949 same embryo
	EMAGE:6948 same embryo
	EMAGE:6946 same embryo
	EMAGE:6947 same embryo
	EurExpress:euxassay_002360 same experiment
	MGI:4823227 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source