EMAGE mouse gene expression spatial database

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EMAGE:6972

Arhgef19 Rho guanine nucleotide exchange factor (GEF) 19 ( MGI:1925912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002434. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002434_01	euxassay_002434_02	euxassay_002434_03	euxassay_002434_04

euxassay_002434_05	euxassay_002434_06	euxassay_002434_07	euxassay_002434_08	euxassay_002434_09

euxassay_002434_10	euxassay_002434_11	euxassay_002434_12	euxassay_002434_13	euxassay_002434_14

euxassay_002434_15	euxassay_002434_16	euxassay_002434_17	euxassay_002434_18	euxassay_002434_19

euxassay_002434_20	euxassay_002434_21	euxassay_002434_22	euxassay_002434_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6972_wholemount_strong.wlz

6972_wholemount_moderate.wlz

6972_wholemount_weak.wlz

6972_wholemount_possible.wlz

6972_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6972_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 weak expression: see section 03 04 06 18 19 20

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 21 22 23

naris

weak

regional

weak expression: see section 10 11 13

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 07 08 09 10 14 15 16 17

nasal cavity respiratory epithelium

weak

regional

weak expression: see section 10 11 12

esophagus

weak

regional

weak expression: see section 12

oral epithelium

weak

regional

weak expression: see section 05 06 07 08 09 11 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5121
Entity Detected:	Arhgef19, Rho guanine nucleotide exchange factor (GEF) 19 ( MGI:1925912)
Sequence:	sense strand is shown >T5121 GACCCACGCGTCCGGGGTGACAGAGTAAAGAAGGAATGGACTGTGGGCCACCTGCAACCCTCCAGCCTCA CTTGGCTGGACCACCTGGCACCGCCCGTCGCCCAGTAGCCGTGTGCCAGCAGGAGAGCCTGTCCTTCGTG GAGCTGCCTCCGAGCCGACTGTGTGTGGATCTCTTTCCCATTGCTCCAGAGGAGCTGCAAGCTCCCGGTA GCCGCTGGTCCCTGGCAACCCCTGCCCCTCTCCAAGGACTGTTATGGCCGACGTCCCCAGGAGGCCCAGA TACCCATATCGCCTCCACCAGTGGAGGAATGAGACCCGGCAGGGCTGGCAGCTGGCCTCACTGTCCTGGT GCCCAGCCCCCAACTCTGGTGGAACCGTGGAGTTCTCAGCACATGCAGCCCCAACGCAGGGCCAGCCACA GTGGCTCAGAGAAGTCAGCCTGGCGCAAGATGCAGGTGTATCATAACGAAGAGGCCACCGGCACCGAGAC CCCTACGGGCCTTCTGGAGACTGGCCAGGCTGCGCAAGAGCAGGCTCTGTGTGCTCTAGAGCCCGGGCCC CAGGAGCTTTCTGGGAACGCCAGAGGGGGCCTTGAGGCACCGGAGCGAAGGCGTTTCTCGGCCTCAGAGC TGATGACTAGGCTGCATTCCTCTCTGCGCTTGGGGCGCAGCACTGCCACCAGGGTTCTGACCTCGGGCTC AGGTGCTGGAGCCATCCGGGAAGGGAAACTGCCCGTGAGGGAATCCCGAAGAGCCGAGACGAGGGCGGAC AGTGTGTCGCTTCCAGGACCCGTCGATCTAAACGAGGTCCACGGTGGTCTGGTGGAACCCAGGCTGGACA CGCAAGACAAGCCGACTCAGGATCCCAGCAGCGCCACTGAGAGGCGCCAGTCCCGCTTCCTCCTTAATTC TGTCCTCTACCAGGAGTACAGCGACGTGGCCAGCGCGCGCGAGTTGCGGCGGCAGCAGCGTGAGGAGGAG GGCCCCGGGGACGGAGCGGAGGGCGCGGAGGAGGGGCCCGGGCCACCGCGCGCCAACCTCTCCCCGAGCA GCTCTTTCCGCGCACAGCGCTCGACGCGGGGCTCCACCTTCTCGCTGTGGCAGGACATCCCCGACGTGCG CGGCAGTGGCGTCCTGGCCACGCTGAGCCTGCGCGACTGCAAGCTGCAGGAGGCCAAATTTGAGCTGATT ACCTCGGAGGCCTCCTACATCCATAGCCTGTCGGTGGCCGTGGGTCACTTCTTAGGTTCCGTGGAGCTGA GCGAGTGTCTGGGGACTCAGGACAAGCAGTGGCTATTTTCAAAACTGCCCGAGGTCAAGAGCACTAGTGA GAGGTTCCTTCACGACCTGGAGCAGCGTCTAGAGGCCGATGTGCTGCGCTTCAGCGTGTGCGATGTGGTT CTACACCATTGCCCCGCATTCCGCAGGGTCTACCTGCCTTACGTCACCAACCAGGCCTATCAGGAGCGCA CCTACCAGCGCCTGCTCCTAGAGAACCCCAAGTTCCCCAGCATCCTGGCTCGCCTAGAGGAGTCTCCTGT GTGCCAGCGGCTGCCTCTCACCTCCTTCCTCATCTTACCGTTCCAGAGGGTTACCCGCCTCAAGATGCTG GTAGAGAACATTTTGAAGCGGACAGCACCGGGCTCCCAAGATGAGGACATGGCCACCAAGGCCTTCAGCG CTCTAAAGGAGCTGGTGCAGGAGTGCAACGCGAGCGTGCAGTCCATGAAGCGGACGGAGGAACTAATTCA CCTAAGCAAGAAGATCCATTTTGAGGGCAAGATCTTCCCGCTGATCTCACAGGCCCGCTGGCTGGTCCGG CACGGGGAGCTGGTGGAGTTGGCCCCCCTGCCCGCCGCACCGCCAGCCAAACTGAAGCTGTCCAGCAAGG CCGTGTACCTTCACCTCTTCAACGACTGCCTGCTGCTGTCCCGGAGGAAGGAGCTCGGGAAGTTTGCCGT TTTTGTCCATGCCAACATGGCTGAGCTACAGGTTCGAGACCTCAGCCTGAAGCTCCAGGGCATCCCAGGC CATGTGTTCCTGCTCAGGCTGCTGCACGGGCAGCGCGCCAGGCACCAGCTGCTGCTGAGAGCACGGACCG AAAGTGAGAAGCAACGGTGGATTTCAGCCTTGAGACCATCCAGCCCCCAGGAGGACAAGGAGATCACATG TGATGGGGAAGACCGCCCCCAGGTCCAGTGTGTGAGGACCTACAAGGCCCTGCAGCCAGATGAACTCACC TTGGAGAAGACAGACATCCTGGCAGTGAAGACCAGGACCAGCGATGGCTGGCTGGAGGGTGTGCGCCTGG CCGATGGTGAGAAGGGATGGGTGCCTCAGGCCCATGTGGTGGAGATCAGCAGCCTCAGCGCTCGACTGCG CAACCTCAGAGAGTACAAGAGGGTCTCCAACGCCAGCAGCAAGCTGGGGGACCCACCTGCCTGATGCTCT GCTGGGGCCCAGCTGGGGCCACTCGGGTGCGGGTGGCCTCTGTGTGTCTGAGGGCCCCCTTCACAGTCAC TGGGGGGTGTCAGTCCTGTAAGTATGCTCTGGCTGCCTCTGCTCACAGTCTGAGCTGGGGCCTTTGCACC GGCTGCCCCATCAGGAGGGCGGGGCTGGCTGTTCCTGCTGTCACTGGTCTCCAGGTGTCCCGTGGGCAGA GGGGCTGCCTCAACCTGCTACTTTGTCCTCCAAGTAGCAAAGTGTCAGATCCAAGCAGGCTTGAATATTA AAGTGACTCTGTGAGTTTTCCTAGTTGCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5340007 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5340007 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6973 same embryo
	EMAGE:6969 same embryo
	EMAGE:6970 same embryo
	EMAGE:6971 same embryo
	EMAGE:6974 same embryo
	EurExpress:euxassay_002434 same experiment
	MGI:4823232 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source