EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6974

Erlin1 ER lipid raft associated 1 ( MGI:2387613)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002503. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002503_01	euxassay_002503_02	euxassay_002503_03	euxassay_002503_04

euxassay_002503_05	euxassay_002503_06	euxassay_002503_07	euxassay_002503_08	euxassay_002503_09

euxassay_002503_10	euxassay_002503_11	euxassay_002503_12	euxassay_002503_13	euxassay_002503_14

euxassay_002503_15	euxassay_002503_16	euxassay_002503_17	euxassay_002503_18	euxassay_002503_19

euxassay_002503_20	euxassay_002503_21	euxassay_002503_22	euxassay_002503_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6974_wholemount_strong.wlz

6974_wholemount_moderate.wlz

6974_wholemount_weak.wlz

6974_wholemount_possible.wlz

6974_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6974_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5157
Entity Detected:	Erlin1, ER lipid raft associated 1 ( MGI:2387613)
Sequence:	sense strand is shown >T5157 CCACGCGTCCGCGGACGCGTGGGGAGAGAGAGAGCCCGGGGCCGGAGCGCGGGGCTTCCCGCCTCAGCCG CCCGCGACCGTACGTGGGCTCTTCCCTGGGTGGGGACTGGCCTGTCACTCAACGGACTGAGGTTCCTCTT CTCCCCATGCTCAGGAGGAACAAGGATGAATATGACTCAAGCCCGGCTTCTGGTGGCTGCAGTGGTGGGG TTGGTGGCGATCCTCCTGTACGCCTCCATCCACAAGATCGAAGAGGGACACTTGGCCGTGTACTACAGGG GAGGAGCTTTGCTAACGAGCCCCAGTGGACCAGGCTATCATATCATGTTGCCTTCCATTACAACATTCAG ATCTGTGCAGACAACACTACAAACGGATGAAGTTAAAAATGTGCCTTGTGGAACAAGTGGTGGAGTCATG ATCTATATTGACCGAATAGAAGTGGTTAATATGTTGGCTCCTTATGCAGTGTTTGACATTGTGAGGAACT ATACTGCAGACTACGACAAGACTTTAATCTTCAATAAAATCCACCATGAGCTGAACCAGTTTTGCAGTGC CCACACACTTCAAGAAGTTTACATAGAATTGTTTGATCAAATAGATGAAAACCTGAAGCAGGCCCTGCAA AAAGATTTAAACACCATGGCCCCAGGTCTCACTATCCAGGCTGTGCGTGTTACAAAACCCAAAATCCCAG AAGCCATAAGAAGAAATTTTGAATTAATGGAGGCAGAGAAGACAAAACTTCTCATAGCTGCACAGAAACA AAAGGTGGTGGAGAAAGAAGCTGAGACGGAGAGGAAAAGGGCTGTTATAGAAGCAGAGAAGATTGCACAA GTAGCAAAAATTCGATTTCAACAGAAAGTGATGGAGAAAGAAACTGAAAAACGCATTTCTGAGATTGAAG ATGCTGCGTTCCTGGCCCGAGAGAAGGCGAAAGCAGATGCCGAGTATTACGCTGCACACAAATACGCCAC CTCAAACAAGCACAAACTGACCCCAGAGTATCTGGAGCTCAAGAAATACCAGGCCATTGCCTCAAACAGT AAGATCTACTTTGGCAGCAACATCCCCAGCATGTTTGTGGACTCCTCCTGTGCTCTGAAATACTCTGATG GTAGGACTGGGAGAGAAGACTCCCTTCCCCCAGAGGAGGCCCGTGAGCCCTCTGGAGAGAGCCCCATCCA AAACAAGGAGAACGCAGGTTGATGCAAGAGGTGGAAATGTTCTCCCATATCAAGATGCGACCCAAGGGGC TAAGTGGGAACAGTGGTTATGTGGACTCGTAAGATTCACAGAGAATGTGTGCTCTGTTGTGATTCTCTTG TCATAGTCCTGGTTTGCCAGCTGACTACAGGATAGACCCAGCTGTCTGGCACTCAAACGGTCTCTGCAGC CACAGTTTTATCAAGTATCCTGTATGTGTTCCTTTGTAAACCGGTACTCATGAATGAGGGAAAGTCTGAT GCTAAGATACTGCCTGCACTGGAATGTCAAACACTATATAACAAGCTGTGGTTTTTAAAAGCTATTGAAT AATGTTTACATTGGTCCCTGAGGACATGTGTGCTCAGACATTCAAGAGCTAGGAGGCCAGAGAGAAGACC TTCAGAAAACGGTAAGTTAAAGAAGACAAGTGTCATCAGACACTTGGGACCCGGGCTCTCTTTAAAGTCT AGTCCCGGCATTCCTCCATGTGATTGACAGCCAGACCTCTGGGTTCCCAGGAAATTATCTTCCAGTCGAA TGACCATTTACTTGATACAAATTGTACCTTTCTGTTTTTCTAGTCAGGTTGGTGGCCTGCAGGGACGCGT ACTTTGCCACCCGACCAGAGGTTCCTCGAAGATATTCCCAATCACTAGTTTATTGCGTTAGGAGACTCAG AGATATAGAAAGCAGCTGAAATTTAAGGGAGATAAAGCCTGCACTGCACCAAAGCTACGGGTCCCTGTGT TTCCTCTATTCAGTGATGTCATCAACCTCACTGTCCCAGCCCATGTGTGACTAAAGTGCCCGGTTTTAGC CACAGACAACTGCTTAGATGTCACCTCTTGGCTGACCAAAGCTGGGACAGGGCTTTAACCAGACATAGGA GCAGTGTGCAATTCCTGATTCACTGCACAGTATTATGTCATAATTGCAGGAATTATTTTTTGTTTTTAAA ACTGGATTTGGGGCACATTCATTCACCCCAACACTTCTATCTAAAGGCCAAGGTTCTAGGGCTGCTATGG TCACTAACACACTGATTCTCCTTAAAGTAATTCTCGAAGTGTGGAACAAAGTGACCGAGACAGCATCCTC AGTCATCTTTGTCTCCTTCCCTGGGATGCAGATACCGAAGTTGCTTTTCCAACTTTCGCCTCCGCTAGGA GATCAGAAAGAATTCTTGTGACTTCCTGGGCAGCCATTGAATTCATTTTCCATGAGAAGATGACAGAGTT AGCCTGTGGCTATAGGAGATCATGTCATCCAGACCTTTTTGCCCATCACATTAACTTTCCTGGAATATTG TGCTGCACAGGTAGACCTGAGTCTGCCCAGCTTGTTGACAGCTCTTGTGTATACTGTGTTGAAGCCAGAC AGAAAAGTAATGGGGCCACTTCTGAAACCTCTCAGCTGTTGATCTCACAGCAGCTAAAGGGTTGTGCCAA ACATTTTATTAAGAAAGTAAAGCCCAGATTTGAATGGGGGTTTTCCCTAGGCCTTATAGTATAGAGGCAT TTGTAATATGGAGAAAATAATTTTTCTCATTTAATTATAGAAATTACCTTCAAACAGATTTTGTGTTCTT TGGCCCTTCAAATACTGGTGTTACATTGTTGCTGCAGATAAATGATGATTGTCGTGGGATATCTGGATCA CTGAGCTCTGTGCTTTCATTCCTAGAGATGTTTCTCATTCCCATTTAGTGAAATGCTGTTGCCCCAAAGT GATGGTTGTGGGATTTCTTACCGGTCATAGGCCCCGGTGAGGAGCAGGGAAGCGCCATTGTGAAAGATTA AAGAAAGCACTTCCACTTGAGCTCCTTATGGAGTGAGCTTCCCTGTGCCCACTCAGTGAACTAAGTCTGA CCATCCTTCAGGGACGTTCCTTTTGGTAAATATACACTGTAATCTTTAAGTCTAAATTTATATGTGAAAG TTAACTTTTTTTAAAAACCTAAATAAAATTATTTTCCTATCAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5345801 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5345801 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6972 same embryo
	EMAGE:6973 same embryo
	EMAGE:6969 same embryo
	EMAGE:6970 same embryo
	EMAGE:6971 same embryo
	EurExpress:euxassay_002503 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source