EMAGE mouse gene expression spatial database

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EMAGE:7024

Golm1 golgi membrane protein 1 ( MGI:1917329)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002445. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002445_01	euxassay_002445_02	euxassay_002445_03	euxassay_002445_04

euxassay_002445_05	euxassay_002445_06	euxassay_002445_07	euxassay_002445_08	euxassay_002445_09

euxassay_002445_10	euxassay_002445_11	euxassay_002445_12	euxassay_002445_13	euxassay_002445_14

euxassay_002445_15	euxassay_002445_16	euxassay_002445_17	euxassay_002445_18	euxassay_002445_19

euxassay_002445_20	euxassay_002445_21	euxassay_002445_22	euxassay_002445_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7024_wholemount_strong.wlz

7024_wholemount_moderate.wlz

7024_wholemount_weak.wlz

7024_wholemount_possible.wlz

7024_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7024_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rectum

strong

regional

strong expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2987
Entity Detected:	Golm1, golgi membrane protein 1 ( MGI:1917329)
Sequence:	sense strand is shown >T2987 TGGCCTCGAGCCAGATTCGGACGAGGAGCCTGAAGAGAGGTCCTACAGGGGTTCACTGTGACTCAACTGT GAAATGGACAGCGAAACGAACGTCGGCCCATTATTGTGGGGTTTAATCAGTACATTTTGCAAAGAAAAAA AAATGGGACCATCTCAGACAGTTTGGGGGTACTTTTAAAGTGTCTACAAGAGATGTCAAAATTGATCAAG CTTTGATTAACAGGTATTTCTTAAGCAGGTCAACACCAAAAAGAGAAATGAGGCTGTCCTGTCTAGGCAG CAGCACCTCCTCACCCTGCAGTGTAAGACAGAGTAGACTGAGGGCAGCTGCTGGCCACAGCGGCTCCCTC CTTGTGCTGCCGCTTGCCAGTTTCTGTCCACAGATATTAAGTAGCTCATCACTCCTGCACACACTTGATT CCACCATCAATGGCCTAGAGCTCTTACCGTCAGCATAAACACCACTAGTCAGTTCTATACAAAAGCTGTT CTGTGCAAAAACTGCATTAATTAAAAGACAGCCACCATATCTGACTTACTTCAGGATTCGGAAGGCTGAG GCAG
Notes:	The probe template was PCR amplified from IMAGE:1533059 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1533059 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7027 same embryo
	EMAGE:7026 same embryo
	EMAGE:7025 same embryo
	EMAGE:7029 same embryo
	EMAGE:7028 same embryo
	EurExpress:euxassay_002445 same experiment
	MGI:4825148 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source