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EMAGE:7029

Pla2g2e phospholipase A2, group IIE ( MGI:1349660)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029
"Pseudo-wholemount" of euxassay_002457. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002457_01 euxassay_002457_02 euxassay_002457_03 euxassay_002457_04
EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029
euxassay_002457_05 euxassay_002457_06 euxassay_002457_07 euxassay_002457_08 euxassay_002457_09
EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029
euxassay_002457_10 euxassay_002457_11 euxassay_002457_12 euxassay_002457_13 euxassay_002457_14
EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029 EMAGE:7029
euxassay_002457_15 euxassay_002457_16 euxassay_002457_17 euxassay_002457_18 euxassay_002457_19
EMAGE:7029 EMAGE:7029 EMAGE:7029
euxassay_002457_20 euxassay_002457_21 euxassay_002457_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7029Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7029_wholemount_strong.wlz
7029_wholemount_moderate.wlz
7029_wholemount_weak.wlz
7029_wholemount_possible.wlz
7029_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7029_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21
meckel's cartilage
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 19 20 21 22
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 moderate expression: see section 05 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3009
Entity Detected:Pla2g2e, phospholipase A2, group IIE ( MGI:1349660)
Sequence:sense strand is shown

>T3009
TGGCTCGAGCAGATTCGGACGAGGCTCACTTCCTCCATTGCAGCAGGCTCCCATGCCCCCTGCCTACCTC
CCCCAGGATGAAACCTCCCATTGCCCTGGCTTGCCTTTGCCTCCTGGTGCCCCTGGCTGGCGGGAACCTG
GTCCAGTTTGGAGTGATGATTGAGAGAATGACGGGAAAGCCTGCCCTGCAGTACAATGACTATGGCTGCT
ATTGCGGTGTCGGTGGCTCCCACTGGCCAGTGGACGAGACGGATTGGTGTTGTCATGCCCATGACTGCTG
CTATGGCCGCCTGGAGAAGCTGGGCTGTGACCCCAAGCTGGAAAAGTACCTCTTCTCTATCACTCGAGAC
AACATCTTCTGTGCTGGTAGAACGGCTTGCCAGCGGCATACCTGCGAGTGTGACAAGAGAGCTGCTCTTT
GCTTTCGCCACAACCTGAACACTTACAACCGCAAGTATGCCCACTACCCCAACAAGCTGTGTACTGGGCC
CACCCCACCCTGCTGAGGCCCTGCTCGGCTCCATAGCCACCCCAGGCTGCTGCAGTCCCAGGCCCAGAGA
Notes:The probe template was PCR amplified from IMAGE:1533423 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1533423 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7027 same embryo
 EMAGE:7026 same embryo
 EMAGE:7025 same embryo
 EMAGE:7024 same embryo
 EMAGE:7028 same embryo
 EurExpress:euxassay_002457 same experiment
 MGI:4827240 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS