EMAGE mouse gene expression spatial database

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EMAGE:7045

Fam129b family with sequence similarity 129, member B ( MGI:2442910)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002553. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002553_01	euxassay_002553_02	euxassay_002553_03	euxassay_002553_04

euxassay_002553_05	euxassay_002553_06	euxassay_002553_07	euxassay_002553_08	euxassay_002553_09

euxassay_002553_10	euxassay_002553_11	euxassay_002553_12	euxassay_002553_13	euxassay_002553_14

euxassay_002553_15	euxassay_002553_16	euxassay_002553_17	euxassay_002553_18	euxassay_002553_19

euxassay_002553_20	euxassay_002553_21	euxassay_002553_22	euxassay_002553_23	euxassay_002553_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7045_wholemount_strong.wlz

7045_wholemount_moderate.wlz

7045_wholemount_weak.wlz

7045_wholemount_possible.wlz

7045_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7045_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5131
Entity Detected:	Fam129b, family with sequence similarity 129, member B ( MGI:2442910)
Sequence:	sense strand is shown >T5131 CCACGCGTCCGAGAGAGCAGCCGAGGCGGCAGCGGGGCTGCGGGGCGACGGCGGCAGCGGGGCCTGCGGA CTCCATGGCGCCAGCGGCGTCAGTCTGAGTAGCGGCGGGCGGATCCGGCCGCGTCTTCTCCTCGCCATGG GAGACGTACTGTCCACACACCTGGATGACGCACGGCGCCAGCACATCGCAGAGAAAACCGAGAAGATCCT CACAGAGTTCCTTCGCTTCTATGAGGACCAGTATGGTGTCTCCCTCTTCAATAGCATGCGCCATGAGATC GAGGGCACCGGGCCACCGCAGGCACAGCTGCTCTGGCGCAAGGTGCCCCTGGATGAACGCATCATCTTCT CCGGAAACCTTTTCCAGTACCAGGAAGACAATAAGAAGTGGAGAAACCGCTTCAGCCTGGTGCCCCATAA CTATGGGCTGGTGCTATATGAGAACAAAGTGGCCTATGAGCGGCAGATACCACCCCGGGCCGTCATCAAC AGCGCGGGCTACAAGGTTCTCACCTCTGTCGACCAGTATCTGGAGCTGGTCGGCAATTCCCTGCCAGGGA CCACATCGAAATCAGGCTCCACCCCCATCCTTAAGTGCCCAACGCAGTTCCCTCTCATCCTGTGGCATCC TTACGCCCGACACTATTACTTCTGCATGATGACAGAAGCGGAGCAGGACAAGTGGCAGGCAGTGCTTCAG GATTGCGTTCGACACTGCAACAACGGGATCCCGGAGAACTCAAAGGTGGAGGGCCCTGCATTCACAGACG CCATCCGCATGTACCGCCAGTCAAAGGAGCAGTACGGCACCTGGGAAATGCTGTGTGGCAACGAGGTGCA GATCCTAAGCAACCTGGTGATGGAGGAGCTGGGCCCAGCGCTGAAGGCAGAGCTTGGCCCACGGCTGAAG GGAAAGCCTCAGGAGCGGCAGCGACAGTGGATCCAGATCTCGGACGCTGTGTACCGCCTGGTATTTGAAC AGGCCAAGGTGCACTTTGAGGACGTGCTGTGTAAGCTGCAGCGGGCCCGGCCGGCCATGGAGGCAGTCAT CCGCACCGACATGGACCAGATCATCACCTCCAAGGAGCATCTGGCCAGCAAGATCCGAGCCTTCATCCTC CCCAAGGCGGAGGTGTGCGTGAGGAACCACGTCCAGCCCTACATCCCATCCATCTTGGAGGCTCTGATGG TGCCCACCAGCCAGGGCTTCACCGAGGTGCGGGATGTCTTCTTCAAAGAGGTCACAGACATGAATCTGAA CGTGATCAACGAGGGTGGCATTGACAAGCTGGGCGAGTACATGGAGAAGCTATCCCAGCTGGCATACCAC CCCCTGAAGATGCAGAGCTGCTATGAGAAGATGGAGCCACTGCGGCTCGACGGGCTGCAGCAGCGATTCG ACGTGTCTAGCACTTCCGTGTTCAAGCAGCGAGCCCAGATCCACATGCGAGAGCAAATGGACAATGCTGT GTACACCTTCGAAACCCTCCTGCACCAAGAGCTGGGGAAGGGACCCACCAAGGAGGAGCTGTGCAAATCC ATCCAGCGGATTCTAGAGCGCGTGCTGAAGAAATACGACTATGACAGCAGTTCTGTGCGCAAGAGATTCT TCCGAGAGGCGCTGCTGCAGATCACCATTCCCTTCCTGCTCAAAAAGCTGGCCCCCACCTGCAAGTCGGA GCTGCCACGCTTCCAGGAGCTGATCTTTGAAGACTTTGCCAGGTTCATCCTGGTGGAGAACACGTACGAG GAGGTGGTGCTGCAGACGGTCATGAAGGACATCCTGCAGGCTGTGAAGGAGGCCGCAGTGCAGCGCAAAC ACAACCTCTACCGTGACAGCATGGTTCTGCACAATAGTGACCCCAATCTACACCTGCTGGCTGAGGGCAC ACCAATCGACTGGGGCGAACAGTACGGTGACAGCGGTGACAGCGGCGGTGGTGACAGTGGTGGCAGCCCC TGCCCCTCTGAAGCAGCCACCCTCACAGAAAAGCGCAGGCGTGCCAAGCAGGTGATGTCCGTGGTCCAGG ACGAGGAGTCGGGGCTGCCCTTTGAGGCTGGCGTTGAACCGCCATCACCTGCATCCCCGGACAGTGTCAC CGAGCTCCGAGGCCTGTTGGCCCAGGACCTGCAGGCTGAAAGCTCCCCACCAGCCAGCCCTCTTCTCAAT GGGGCTCCAGTCCAAGAGAGCTCCCAACCCGTGGCAGTCCCAGAGGCCTCACCGCCAGCCTCACCCCTCC GGCATCTCCCACCTGGAAAGGCTGTGGACCTTGAGCCCCCTAAGCCCAGTGACCAAGAGACCGGAGAGCA GGTGTCTAGCCCCGGTAGCCGTCCCCCCATCCATACCACCACTGAGGACAGTGCGGGAGTGCAGACGGAG TTCTAAGCATGGCCTCCAGGCTTCTGTTGGACTCTGCACCAGGGCGCTCCTCCTTGGGCACCACAGCCTC TCACCTCTGGGGAGGGGTACTCCAGTTTGTGCCTTGTGGGAAAGGTGGCTGGGGTGGTGTGGCATCTCCA GGGGTCTGGCCTACCCAAGTCACTTTATGGGGTTCAGTACACCTGTTACTGCCCCGTTTTCTGTGGGCTG ATCTCCAATGCTGGGTCTGGTTTTGAGGGTTTCCTCTTTGTGCAAGGGTCTGGGGAGCCCCTCCATCCCG CCCTCGTACTGTTACGGCGGCAGATGAAAGCAGTGGTGGTGTGACTCACGTATAGGAAGCTAGGAAGCTG TCATCTCACTCTGGGGCTCCTTGGGACCAACGTGGCAAGGAAAAGGCAGACCAGGCCTCTGTCCCGCCCA CTCCCTGCAGGTCCCAAGGTTAGACACTGGCCTCACTTTGCTAGCTACTGCTGTGGGAGGGCGGGGCTTA GGGGTCTTACAGGGGAGTGATGCTAGAGGTCAGAGGTCATGATGGAATGAGAAAGGCCCAGAATGTGTTA GTCTGTGCAGGGGAAGTGTCCTGAGGGGGAGGGAGCTTGGGAGTGTCTTTGCCAGGATGGCAAAGTGAAT AGCTGAAGTTGGAGTGTCTTGGGTGACCCGTTCCTGCTCATCTGGCTGACAACGCTCATTGGCAGGGCTT AGCTGTGGGCTGTTTCACTACGTGCCAGGAAGCTGGTGGGGCTCCAGAGGGACATTGAGGGCTATGGCTC TCTGAGACACTGCTGACCATTCATGGCCTCTGCTCCCGTCACCTAATCCTGATAATTCCAGACCCCTCTT CCAATGTCCTTGTGAGCCATTTGGGCTTGTCTTTCCCTCTTAGTCACAAGGGTCAAACCAAGAGTCAGTT AGCTAGATGACTCTCCCCAGACTCTGTCGCCAGAGTTGGGAGAACAAAGCAGAGACTGCCGCTCCAAGGC CCCCAGGACGAGGTCCCTCCCAGTCCTGTGTCCCTTGCTCTGTGGACCTGCTCCCCAGACGCAGGCCTCA GACCCACAGGATGTGGCCGGTGCTCAGGGACACCCAGTCCCCTGCCCTCACTCCGAGTACTGACTTCTTT CCATGTTCTCTAGTGCCTTGGGGGTTTCCACCATCCTTCCAGATATGGTATGAAGAATCTATGCAAACTG TGTAAGCTTCTGCTCATCAATAAACGCTTTATTTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5343013 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5343013 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7043 same embryo
	EMAGE:7042 same embryo
	EMAGE:7047 same embryo
	EMAGE:7044 same embryo
	EMAGE:7046 same embryo
	EurExpress:euxassay_002553 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source