EMAGE mouse gene expression spatial database

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EMAGE:7047

Gusb glucuronidase, beta ( MGI:95872)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002565. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002565_01	euxassay_002565_02	euxassay_002565_03	euxassay_002565_04

euxassay_002565_05	euxassay_002565_06	euxassay_002565_07	euxassay_002565_08	euxassay_002565_09

euxassay_002565_10	euxassay_002565_11	euxassay_002565_12	euxassay_002565_13	euxassay_002565_14

euxassay_002565_15	euxassay_002565_16	euxassay_002565_17	euxassay_002565_18	euxassay_002565_19

euxassay_002565_20	euxassay_002565_21	euxassay_002565_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7047_wholemount_strong.wlz

7047_wholemount_moderate.wlz

7047_wholemount_weak.wlz

7047_wholemount_possible.wlz

7047_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7047_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 19 20 21 22

pancreas

moderate

regional

moderate expression: see section 09 10 11 12

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5108
Entity Detected:	Gusb, glucuronidase, beta ( MGI:95872)
Sequence:	sense strand is shown >T5108 CCCAGCCGCTGGTCGGTGGGCAGCCTGGCCAGTATGTCCCTAAAATGGAGTGCGTGTTGGGTCGCGCTGG GCCAGCTGCTGTGCAGCTGCGCGCTGGCTCTGAAGGGCGGGATGCTGTTCCCGAAGGAGAGCCCGTCGCG GGAGCTCAAGGCGCTGGACGGACTGTGGCACTTCCGCGCCGACCTCTCGAACAACCGGCTGCAGGGTTTC GAGCAGCAATGGTACCGGCAGCCGCTACGGGAGTCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCT TCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAAT CCTGCCACGGCGATGGACCCAAGATACCGACATGAGAGTGGTGTTGAGGATCAACAGTGCCCATTATTAT GCAGTTGTGTGGGTGAATGGGATTCATGTGGTGGAACATGAGGGAGGTCACCTCCCCTTTGAGGCTGACA TTAGCAAGCTGGTCCAGAGTGGGCCCCTGACCACCTGCCGGATCACGATTGCCATCAACAACACACTGAC CCCTCATACCCTTCCGCCGGGGACCATCGTCTACAAGACTGACACCTCCATGTATCCCAAGGGTTACTTT GTCCAGGACACAAGCTTTGACTTCTTCAACTATGCGGGACTGCATCGATCTGTGGTCCTCTATACCACCC CTACCACTTACATCGATGATATCACTGTGATCACTAATGTGGAGCAAGACATCGGGCTGGTGACCTACTG GATTTCTGTGCAGGGCAGTGAACATTTCCAGCTAGAAGTGCAACTTTTGGATGAGGGTGGCAAAGTCGTG GCCCATGGGACAGGGAACCAGGGTCAACTTCAGGTTCCCAGTGCCAACCTCTGGTGGCCTTACCTGATGC ATGAGCATCCAGCCTACATGTACTCCTTGGAGGTGAAGGTGACAACAACTGAGTCTGTGACTGACTACTA CACCCTTCCTATCGGGATTCGAACAGTGGCTGTCACAAAGAGCAAGTTCCTCATAAACGGGAAGCCCTTC TATTTCCAAGGGGTCAATAAGCACGAGGATTCAGATATCCGAGGGAAAGGCTTCGACTGGCCGCTGCTGG TAAAGGATTTCAACCTGCTCCGTTGGCTCGGGGCAAATTCCTTTCGTACCAGCCACTATCCCTACTCAGA GGAGGTACTTCAGCTCTGTGACCGATACGGGATTGTGGTCATCGATGAGTGTCCCGGTGTGGGCATTGTG CTACCTCAGAGTTTTGGCAACGAGTCACTTCGGCACCACCTAGAGGTGATGGAGGAGCTGGTTCGCCGGG ACAAAAATCACCCTGCGGTTGTGATGTGGTCTGTGGCCAATGAGCCTTCCTCTGCTCTGAAACCCGCCGC ATATTACTTTAAGACGCTGATCACCCACACCAAAGCCCTGGACCTCACCCGTCCCGTGACCTTTGTGAGC AACGCCAAATATGATGCAGACCTGGGGGCCCCGTACGTGGATGTTATCTGTGTAAACAGCTACTTTTCTT GGTATCATGACTATGGGCATTTGGAGGTGATTCAGCCACAGCTGAATAGCCAGTTTGAGAACTGGTATAA GACGCATCAGAAGCCGATTATCCAGAGCGAGTATGGAGCAGACGCAATCCCAGGGATCCACGAGGACCCG CCTCGCATGTTCAGTGAGGAGTACCAGAAGGCTGTTCTGGAGAATTACCATTCAGTTCTGGATCAGAAAC GTAAAGAATACGTGGTCGGAGAGCTCATCTGGAATTTCGCCGACTTCATGACGAACCAGTCACCGCTGAG AGTAATCGGAAACAAGAAGGGGATCTTCACTCGCCAGAGACAGCCCAAAACTTCGGCCTTTATTTTGCGA GAGAGATACTGGAGGATTGCCAACGAAACCGGAGGTCACGGTTCAGGGCCGAGAACCCAGTGTTTCGGAA GCAGACCGTTCACGTTCTAAAGTTACAACTACCTCACTGCAGACTCAAAGGTGTCCCCAGACACTTCCTG ACACTAGTGTTTCTATTTGGGATTTTGTGAACTGATACAAGGGGACTTTAGAAGTGACATCAAAGCTTTT CTTCCGTGGGGATAATAACTTACAGTGCCTTAGGCTCAGTAATATGTCCTGCTGAGAGGTGTCCAAGATA ATTTGTTAGTTTTTTGGCCTTGGCTTTGTGAACTCTTGAAAGCCTGCTGTGTGAACATTCTCACTAAATT AAGAGCTACTGGTGAGAGGCTGGAGTGAAGGCTGGCTCACAATTTAAGAGCACTGACTGTTCTACTGGAG GTTCCAGATTCAATTCCTAGCACCTACCTGGTGGCTAACTATGAGCTGGTCCTCCATTTCCCAGGTGATC CAAATGCCCTTTTGGCCCCTGCGGGTACCACATGTATGTGGTATGTGGATCTACATGTGGGGAAAAAAGT CATACATGCAAAATAAAAATGAATCTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5325429 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5325429 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7043 same embryo
	EMAGE:7045 same embryo
	EMAGE:7042 same embryo
	EMAGE:7044 same embryo
	EMAGE:7046 same embryo
	EurExpress:euxassay_002565 same experiment
	MGI:4825288 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source