EMAGE mouse gene expression spatial database

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EMAGE:7116

Atp5l ATP synthase, H+ transporting, mitochondrial F0 complex, subunit g ( MGI:1351597)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002590. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002590_01	euxassay_002590_02	euxassay_002590_03	euxassay_002590_04

euxassay_002590_05	euxassay_002590_06	euxassay_002590_07	euxassay_002590_08	euxassay_002590_09

euxassay_002590_10	euxassay_002590_11	euxassay_002590_12	euxassay_002590_13	euxassay_002590_14

euxassay_002590_15	euxassay_002590_16	euxassay_002590_17	euxassay_002590_18	euxassay_002590_19

euxassay_002590_20	euxassay_002590_21	euxassay_002590_22	euxassay_002590_23	euxassay_002590_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7116_wholemount_strong.wlz

7116_wholemount_moderate.wlz

7116_wholemount_weak.wlz

7116_wholemount_possible.wlz

7116_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7116_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

moderate

regional

moderate expression: see section 04 05 06 07 08 20 22 23 24

medulla oblongata basal plate

moderate

regional

moderate expression: see section 09 10 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23

ventral grey horn

moderate

regional

moderate expression: see section 11 12 13 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5229
Entity Detected:	Atp5l, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit g ( MGI:1351597)
Sequence:	sense strand is shown >T5229 CGGACGCGTGGGTTCAGCCGGCGGTTCGGGGAGACGTACCTTCCACCTTAGACCATGGCCAAGTTCATCC GTAACTTCGCGGAGAAGGCACCGTCGATGGTAGCCGCTGCCGTGACTTACTCGAAGCCTCGATTGGCCAC ATTTTGGCACTACGCCAAGGTTGAGCTGGTTCCCCCAACCCCTGCTGAAATCCCTACAGCTATTCAGAGT GTGAAAAAAATCATTCAAAGTGCTAAAACTGGTAGCTTCAAACACCTTACAGTTAAGGAAGCTGTGCTGA ATGGTTTGGTGGCCACTGAGGTGTGGATGTGGTTTTATATCGGAGAGATCATAGGCAAACGTGGCATTGT TGGCTATGATGTTTGAAGACCAATCTTTAACTTCTGATTTGAGTTCTTATTTGAATGTTCTTGGACCATG TGTAACAGGACTGCTATCTGAATAAAATACTAGGTGTTGAAAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5356491 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5356491 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7117 same embryo
	EMAGE:7120 same embryo
	EMAGE:7115 same embryo
	EMAGE:7118 same embryo
	EMAGE:7119 same embryo
	EurExpress:euxassay_002590 same experiment
	MGI:4823316 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source