EMAGE mouse gene expression spatial database

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EMAGE:7133

Zfp418 zinc finger protein 418 ( MGI:2444763)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002678. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002678_01	euxassay_002678_02	euxassay_002678_03	euxassay_002678_04

euxassay_002678_05	euxassay_002678_06	euxassay_002678_07	euxassay_002678_08	euxassay_002678_09

euxassay_002678_10	euxassay_002678_11	euxassay_002678_12	euxassay_002678_13	euxassay_002678_14

euxassay_002678_15	euxassay_002678_16	euxassay_002678_17	euxassay_002678_18	euxassay_002678_19

euxassay_002678_20	euxassay_002678_21	euxassay_002678_22	euxassay_002678_23	euxassay_002678_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7133_wholemount_strong.wlz

7133_wholemount_moderate.wlz

7133_wholemount_weak.wlz

7133_wholemount_possible.wlz

7133_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7133_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5302
Entity Detected:	Zfp418, zinc finger protein 418 ( MGI:2444763)
Sequence:	sense strand is shown >T5302 GTTTAACTGTCCATGGGTTGGTATTTGGAGGATTCTGAGCTTTAAAATCAGCAAGTTAGACTAAAGGTAC GAGAGCGAACAGGCACTGGGTGTCGGCCAAGCAGACCATCATCAGCCGCGGCGGCCGTGCACCGGCCCCT GCGTACTCTACAGAGCCGGATGGCGGCGCTGAAGCACCTGGCTCAGGTTCCCAGATCTACAGAAAAGGGT ATGGTCTGTATACAGGGCTGTGTAACCTTTGAGGACGTGGCAGTTTACTTCTCCCAGGAAGAGTGGGAGC TCCTGGATGAATCTCAGAGGCTCCTGTATCTTGATGTGATGCTGGAGAATTTTACCCTTATAACCTCACT GGCTCATTGGTCTGAAGCAGAGGCTGAAGACAAAACCTGTGCACCACAGATCAGGACTGCAAAGGAAGGT CTACCCACGAAGAAGCCCCATCCCTCTGACATCTGTAGCACCGTCCTTAAAGATATCTTGCATCTAAGTG ACCTACCCGGGCAGAAACCACATTTGACTGAGGTGTGTACAGACCTCCTGGACCAGAAGCATCACAGAGC CAAGAATTGGTTAAAGAAAGATGTGGACTCCCTTGTGAAGAACTGTATATTCCATGTAGCAGGGAACCCT TCTGTCTGTAGTAAGATTGGCGAGAAATTCCCAGCTGTATGGAATCTTCTCCAACCCAAGGCCATTCCCA AAGGTGAGAAACAAAATCAAATTAAGTGTAGAAAGGCTTTTCACTCAGAGAAAAATAACTCTAAGTCAGA TAAATATAACAAATCTTCCAGCCCCCAACACAGGCTTCATGAATATCCAAGACTTTGCAGTGGAAAGGAG GGGTTTGAGTCTAACAGTTGTGAGCAAGACTTAAACAAGTACTCGATTGCACCATCCCAGACAGATCAGA CTGAAAACCGACCATATGGGTGTCATGACTGTGGAAAATGGTTTGGCCAGAAAGCCACACTTAGAATACA CCAGAGACGCCATACCGGAGAAAAACCGTATAGATGTGGTGAATGTGGAAAGTCTTTTTGTCAAAGCTCT AACCTAAGCGAACACTGTAGAGTTCATAGTGGAGAAAGACCTTTTGAGTGTCTGGAATGTGGGAAAGCCT TCGGGTGCCATTCTAGTCTTCTGCGACATCAGAGAACACACACAGGAGAATGGCCATATGAATGTGGTGA CTGCGGGAGGCTGTTTAGGCAGATTGTCAGCCTGATTACACACCAGAGGACTCACACTACAGAGAAGCCT TACGAATGTGGACAGTGTGAAAAGTCTTTTAGTCACAAAGCCACGCTTACTGTACATCAGAGAGTTCACA CTGGAGAGAAGCCCTATCACTGTGAAGCATGTGGGAAATCCTTCAGCCAAAGCGCCAACCTTATTAAACA CTCCAAAATTCACACTGGAGAAAAGCCTTATAAGTGTGGGGAATGTGGTCTATGCTTTCGCCAGAGAGCT ACCCTCATGAAACACCAGAGAACCCACACCTCAGAAAGGCCTTATGAATGTAGAGAATGTGGTAAGTTCT TTAAACAGTACTTCTACCTCATTGAACACAGTAGGATTCACACCACAACAGAATTTTATGAGTGTGGACA GTGCGGGAAATCTTATACCCAAAACGCCACCCTCATCAGACACCAGCGAGTCCATACAGGAGAGAGTCCT TATAAGTGTAAGGAGTGTGGAAAAGCCTTTGAATACAAATCCAGACTTAATCGACATCAAAGAACTCACA CTGGTGAAAGGCCTTATGAGTGTGCCAAGTGTGGGAAATTCTTCAGGGAAAGCTACAATCTTGCTGAACA CCAGAAAATTCACACGAAAGCAAAGCCTTACAATTGTGATCAGTGTGGGAAGTGTTTTAGCCGGAGAGCT GACTTGGTTAAACACCAGAGAGTTCACACTGGTGAAAGGCCTTATACATGTGGTGAATGTGGGAAGACCT TCAGCCGAACTACCAACCTTGTTCAACACAGAAGAATTCACACTGGGGAACGGCCATACGAGTGTGACCA GTGTGGGAAGTCTTTTAGCCAGGTGTCTACCCTCACTAGGCATCAGCTACTTCACACTGGAGAAAAATCT TATAAATGTAGCAAATGAAGGAAAGCCTAGACAACTCTCTAGCATCTTCTAACACCAAAAGATAGACCAT CATAATTCCTGGAAGCCAACCCTTGAAAGCACCCTCAGGCCTCGAAAGATGTTGCATTGTTTTTCACTGT AACAAACTAGAGAGCCTCATAGAGTTCTAGGCCGTGTAACTTGTACTAGAGAGCTAGTCTGCATGTTCTA GACAGTCTGTTTCCATTGCCAGAATTAAATAGCTGCCATAATGTCTCATAGAAACTGTCACTCCTCTAGC ACCTTGCCCTGAGTGTATATCCCCCCAACATGCCCATATTCTCCCCTTCCCTAATGGAAGCTTCAACTTC CTGTGCCTTGCAAATGACCTTCATCCCCTTTTCTCACTGAATATGTATGGGTCTGTATTTTGTCTAAACG AACCTGGTCTGGGATTAGATGGAGGCTCCATCAAGGAATATCTTCTCAAGTCTTTGTCTCACATGATTCA TGTGGCTGTTTACATGATAGGAACTAGCCAGAGACTTCCTGCCATGTTAATATGCAATAATAAAAGACCT TATGGATTTTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5363178 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5363178 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7137 same embryo
	EMAGE:7138 same embryo
	EMAGE:7136 same embryo
	EMAGE:7135 same embryo
	EMAGE:7134 same embryo
	EurExpress:euxassay_002678 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source