EMAGE mouse gene expression spatial database

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EMAGE:7134

Lrrc41 leucine rich repeat containing 41 ( MGI:2441984)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005223_01	euxassay_005223_02	euxassay_005223_03	euxassay_005223_04

euxassay_005223_05	euxassay_005223_06	euxassay_005223_07	euxassay_005223_08	euxassay_005223_09

euxassay_005223_10	euxassay_005223_11	euxassay_005223_12	euxassay_005223_13	euxassay_005223_14

euxassay_005223_15	euxassay_005223_16	euxassay_005223_17	euxassay_005223_18	euxassay_005223_19

euxassay_005223_20	euxassay_005223_21	euxassay_005223_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7134_wholemount_strong.wlz

7134_wholemount_moderate.wlz

7134_wholemount_weak.wlz

7134_wholemount_possible.wlz

7134_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7134_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5300
Entity Detected:	Lrrc41, leucine rich repeat containing 41 ( MGI:2441984)
Sequence:	sense strand is shown >T5300 AGCAACGACCATGGAGGCCACGTCCCGGGAGGCGGCGCCAGCGAAGAGCTCGGCCTCGGGCCCCAGCGCT CCCCCCGCCCTGTTCGAGCTGTGCGGGCGGGCAGTGAGCGCCCATATGGGGGTTCTGGAGAGCGGGGTGT GGGCCCTCCCAGGCCCAATACTTCAAAGCATCTTACCTCTGCTCAATATATATTACTTGGAGAGGATTGA GGAAACTGCTCTCAAAAAAGGTCTCTCTACTCAAGCCATCTGGCGCCGACTCTGGGATGAACTGATGAAG ACAAGGCCTTCCAGTTTGGAGAGTGTGACTTGCTGGCGAGCCAAGTTTATGGAGGCCTTTTTTTCCCATG TTCTACGTGGAACCATTGATGTGTCTTCTGATAAACGTCTTTGTGATCAGCGCTTCTCACCTCTCCTGCA CAGCTCCCGTCATGTCCGACAGCTAACCATCTGCAACATGCTGCAGGGAGCAACTGAGCTAGTGGCAGAG CCCAACCGTAGGGTTCTAGAGACCCTGGCCAGTTCTCTGCATACCCTCAAGTTCCGCCACCTGCTGTTCT CTGATGTGGCTGCTCAGCAGTCACTTCGACAGCTGCTGCATCAGCTCATCCACCATGGGGCTGTTAGCCA AGTGTCGCTGTACTCCTGGCCTGTGCCCGAGTCAGCCCTTTTCATCCTTATTCTTACCATGAGTGCTGGC TTTTGGCAACCAGGCCCAGGTAGCTTGCCTTGCCGCCTCTGTGGTGAAGCCTCCCGAGGCCGGGCCCCAT CTAGAGATGAAGGGTCCTTATTGCTAGGCTCTCGCAGGCCTCGGCGTGATGCAGCAGAGCGATGTGCTGC TGCACTAATGGCCACCCGGCGGAAGAGTGAAGTCAAGCAGATGCCCAGGGCTGTACCTCCCACTCGTGTA ACACGCCGCAGCACACAAGAGAGCCTGGCAATAGGTGGAACAGACTCTAAGTTGTACCTCCCAGCCACCT CTTATGAGGCTTCTGGCACAAAACAGCCATCTGCTCCAGCTGCAGCCTCTGCCTCCTCTTCCACATCATC CAAACGGGCTCCAGCTAGCTCAGCCTCACAGCCTAAGCCCTTAAAGCGTTTCAAACGAGCTGCAGGGAAG AAAGGGCCCCGCACCCGTCAGGGGTCTGGTGCAGAGTCTGAAGACCTGTATGACTTTGTTTTTATTGTGG CTGGTGAGAAGGAGGATGGTGAAGAGATGGAGATTGGGGAAGTAGCTTGTGGAGCTCTGGATGGGTCAGA TCCCAGCTGTTTGGGGCTCCCAGCACTAGAGGCATCCCAGCGATTCCGCAGCATTTCCACCTTGGAGCTA TTCACAGTTCCTCTCTCCACGGAGGCAGCTCTGACACTGTGCCATCTTCTGAGTTCCTGGGTATCACTGG AGAGCCTTACACTTTCCTACAATGGTCTGGGCTCTAATATCTTCCGCCTCCTGGACAGCCTACGGGCCCT GTCAGGCCAGGCTGGATGTCGCCTCCGTGCCCTGCATCTCAGTGACCTTTTCTCACCACTGCCCATCCTG GAATTGACCCGTGCCATTGTGCGAGCCTTGCCCCTGTTGCGGGTCCTGTCTATTCGTGTTGACCACCCAA GCCAGAGGGACAACCCTGCTGTACCAGAGAATGCTGGGCCCCCTGGACACATAGTTGGGGATGAAGAAAT ACCAGAAAACTGCCTCGAGCAGTTGGAAATGGGATTTCCACGAGGAGCCCAGCCAGCCCCACTGCTCTGC TCTGTCCTCAAGGCCTCAGGTTCTCTACAGCAGCTGTCACTGGACAGTGCCACCTTTGCCTCGCCTCAAG ATTTTGGGCTTGTGTTGCAAACACTCAAAGAACACAACCTATCCCTGAAGAGGCTGAGCTTCCATGATAT GAATCTTGCAGACTGTCAGAGTGAAGTGCTTTTTTTGCTAAAGAATCTGACCCTTCAAGAGATTACCTTC TCCTTCTGCCGTCTGTTTGAGAAGCGCCCAGTCCAATTTCTTCCTGAGATGGTTGCTGCTATGAAGGGCA ACTCTACACTGAAGGGCCTTCGACTACCAGGGAACCGCCTGGGGAACGCTGGCCTATTAGCCCTAGCAGA TGTTTTCTCAGAGGATTCTTCCTCTTCTCTCTGTCAGCTGGACATCAGTTCCAACTGCATCAAGCCAGAT GGGCTTTTGGAGTTTGCCAAACGACTGGAGCGCTGGGGCCGAGGAGCCTTTGGTCACCTACGCCTCTTCC AGAACTGGCTGGACCAGGATGCAGTCACAGCAAGAGAAGCAATCCGGCGACTCCGGGCTACCTGCCACGT GGTTAGCGACTCATGGGACTCAACCCAGGCCTTTGCAGATTATGTCAGCACCATGTGATGGGGCCCATAC CTCACAGGCCCATCCTCAGTACCATCAGCTTGCAGGGACTGAGCCGTGGGCTGCCCAGAATCCCCAACCA CTGATTCTGTCATCCTCTTTCTGCCACTTTGTCTATTTCTGTTTCCTTTTCATCTTCCCTTGCACTGAGG TCCTGGAGGCCTTGATGAGGCCCAGCAAAGGCATTCCCACCGCTGAGTTCAGAGGCTTTGGGCCACTTAC TCAGTACTCTGGGAACCCCAGGCCAGGAGGTTACGGGTCTCTATCGTCACTGAGGAAAACACCCCCTACC CCTGCTCTGGGGTTTCCTGCTTCATTCCTCAAGCAGGCACCCAGGCTTTAGAGAAGTATAGGAATGCCTA ACACCAGGCCTCTTCCCTCCTGGGCTTACCAATGTGTTCCCAGGCCTCAGTCCCTTTCATGCCTTTATTC CTTTCTTTTTCTTTTTTTTTTTTAAACCAAAAAAGTTTTTCTTATAAAATAAATTTTGGGCAAACATCAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5362925 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5362925 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7133 same embryo
	EMAGE:7137 same embryo
	EMAGE:7138 same embryo
	EMAGE:7136 same embryo
	EMAGE:7135 same embryo
	EurExpress:euxassay_005223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source