EMAGE mouse gene expression spatial database

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EMAGE:7213

Lzts2 leucine zipper, putative tumor suppressor 2 ( MGI:2385095)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002720. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002720_01	euxassay_002720_02	euxassay_002720_03	euxassay_002720_04

euxassay_002720_05	euxassay_002720_06	euxassay_002720_07	euxassay_002720_08	euxassay_002720_09

euxassay_002720_10	euxassay_002720_11	euxassay_002720_12	euxassay_002720_13	euxassay_002720_14

euxassay_002720_15	euxassay_002720_16	euxassay_002720_17	euxassay_002720_18	euxassay_002720_19

euxassay_002720_20	euxassay_002720_21	euxassay_002720_22	euxassay_002720_23	euxassay_002720_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7213_wholemount_strong.wlz

7213_wholemount_moderate.wlz

7213_wholemount_weak.wlz

7213_wholemount_possible.wlz

7213_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7213_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5895
Entity Detected:	Lzts2, leucine zipper, putative tumor suppressor 2 ( MGI:2385095)
Sequence:	sense strand is shown >T5895 GCCCCGGAGGCCCCTGGCCAGACCTGAGCCTCTGCCACCATGGCCATTGTGCACACTCTGCCAGTGCCTC TGGAGCCCGCACGTGAGACAGCCACTGCCCCCAAAACTCCAGCAATGGGCAGCGTGAGCAGTCTTATCTC AGGCCGGCCCTGCCCCGGGGGACCTGCTCCTCAACGACATCATGGTGTCCCTGGGCCCACCTTCTTCCGC CAGCAGGATGGGCTGCTACGGGGTGGCTACGAGGCACAGGAACCGCTCTGCCCGGCTGTGCCACCGAGGA AGGCTGTCCCAGGCAATAGCTTTACCTATGTCAATGAGGACTTCCGGACAGAGTCTCCTCCCAGCCCCAG CAGTGATGTTGAAGATCCCCGGGAACATCAGGCACACAATGCCCACCTCCGTGGGCCCCCACCAAAGCTC ATCCCTGTCTCTGGAAAGCTGGAGAAGAATATGGAGAAGATCCTGATCCGGCCAACAGCCTTCAAGCCAG TGCTACCCAAACCTCGGGGGGCGCCATCCCTACCCGGCTTCCTTGGTCCTCGAGCTGCTGGGCTATCTGG GAGCCAGGGCAGCCTGACACAGCTGTTTGGGGGGCCAGCCTCTTCCTCCTCCTCTTCCTCTTCCTCCTCT GCTGCTGACAAACCCTTGGCACTGAGTGGTTGGGCCAGTGGCTGCCCTTCAGGAACGCTGTCAGACTCTG GCCGAAATTCTTTATCCAGCTTGCCCACCTACAGCACTGGAGGTGCCGAGCCAACCACCAACTCCCCAGG TGGGCACCTGCCCTCCCACGGCCCTGGCCGAGGGGCACTGCCTGGTCCAGCCCGAGGGGTCCCTACTGGG CCCTCCCATTCAGACAGTGGCCGGTCCTCCTCCAGCAAAAGCACGGGCTCCTTGGGGGGTCGTGTAGCTG GGGGGCTCTTGGGCAGTGGTGCCCGGGCCTCCCCTGGCAGCAGTTCTGGTGGAGACCGCTCTCCACCCCC TCCACCACCTCCACCCCCTTCGGATGAGGCCTTGCTACAGTGTGTCCTGGAAGGGAAGCTCAGAGACCGC GAGGCAGAGCTGCAGCAGCTGCGGGACAGTATGGATGAGAGTGAGGCCACAGTGTGTCAGGCATTCGGGG CACGGCAGCGGCGCTGGCCACGGGAGCGAGGGGAGGACTGTGCAGCCCAGGCGCAGCAGGCCACGCAGCG CGTCCAGCGGGCCCAACAGCTGCTGCAGCTGCAGGTGTTCCAGCTGCAGCAAGAGAAGCGCCAGTTGCAG GATGACTTTGCCCAGCTGCTACAGGAGCGGGAACAGCTGGAGCGCCGCTGTGCCACTTTTGAACGGGAGC AGCGGGAACTCGGGCCACGGCTGGAGGAGACCAAATGGGAGGTGTGTCAGAAATCAGGTGAGATCTCCCT GCTGAAGCAGCAACTGAAGGAATCTCAGGCAGAGCTGGTCCAGAAGGGCAGTGAGCTGGTGGCGCTGCGG GTGGCCCTCCGTGAGGCCCGGGCCACCTTGCGGGTCAGTGAGGGCCGTGCCAGGGGCCTGCAGGAGGCAG CCCGAGCTCGGGAGCAGGAGCTGGAGGCCTGCTCGCAGGAGCTTCAGCGGTACCGCCAGGAGGCTGAGCG GCTCCGGGAGAAGGCCGGGCATTTGGATGCCGAAGCATCGGGACTCCGGGATCCCCCTGTACCCCCTGCC ACCACCGACCCGTTCCTCCTGGCAGAGAGTGATGAGGCCAAGGTGCAGCGGGCAGCGGCTGGGGCAGGGG GTAGCCTGCGGGCTCAGGTGGAACGGCTGCGCCAAGAACTGCAGCGGGAGCAGCGGCGAGGGGACGAGCA ACGGGACAGCTTTGAGGGGGAGCGGCTGGCCTGGCAGGCAGAGAAGGAACAGGTGATCCGCTACCAGAAG CAGCTGCAGCACAACTATATCCAGATGTACCGGCGTAACCGGCAGCTGGAGCAGGAACTGCAGCAACTGA GCCTGGAGCTGGAGGCCCGCGAGCTTGCGGATTTGGGCCTGGCGGAGTCGGCCCCTTGCATCTGTCTGGA GGAGATCACTGCCACTGAGATCTAGGGCTCTGCAAGGGGTCTGAGCCACAGAGGCCCACCTGCCCGGGGA TCCACTGAGCACGCGCTCCAGGTCTCAGAGCCAAAGGGCCTCGGCCGAGCATTCGGGTGGCTCCGTAACT TGGAGAAGCAGCAGAATCAACCCTCTGTGTCACCGTCACCCAGAGGCTCCTGCCTACTCTGCCATTTCAC TCCCTCACTGTCGCCACTGCCAGCCCACTCCAGCTGGGAAGGGAAAGAGGCTGCTTCCCTGTCTCCGCAT CTACCTGCCTGAAGCCAGAGAGAGCCCGAGGGCAACCAGCTGCTCCAGATGTGCCTGCCCTCAACATGTC CACGTGGAGGGGCAGGGCCGGGCATGGGGCTGATCCCCATGTTGAGCTCTGCAAGCCTCTTCTGGGCTGA GGGGGCTGAAGTCAGACCAAAGGAAGAACCCAGCAATGTCTTGTTTATTTGTGTTTGTGACCAAGCCGTC CTCCCTAGTTCCAGGGTTACGGCCTCATTTCCACTTGTATATTTTTCACACTGTAAATTTCTTGTACAAA CCCAAAGGGGAAAAAAATTTAAAAAAAAAAAAAAGTTTTGTTTTAAAAAAAAAAAGTGCAATGGATAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3157493 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3157493 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7211 same embryo
	EMAGE:7209 same embryo
	EMAGE:7212 same embryo
	EMAGE:7208 same embryo
	EMAGE:7210 same embryo
	EurExpress:euxassay_002720 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source