EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7226

Mlycd malonyl-CoA decarboxylase ( MGI:1928485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002721. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002721_01	euxassay_002721_02	euxassay_002721_03	euxassay_002721_04

euxassay_002721_05	euxassay_002721_06	euxassay_002721_07	euxassay_002721_08	euxassay_002721_09

euxassay_002721_10	euxassay_002721_11	euxassay_002721_12	euxassay_002721_13	euxassay_002721_14

euxassay_002721_15	euxassay_002721_16	euxassay_002721_17	euxassay_002721_18	euxassay_002721_19

euxassay_002721_20	euxassay_002721_21	euxassay_002721_22	euxassay_002721_23	euxassay_002721_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7226_wholemount_strong.wlz

7226_wholemount_moderate.wlz

7226_wholemount_weak.wlz

7226_wholemount_possible.wlz

7226_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7226_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 09 10 11 18 19 20

dorsal root ganglion

weak

regional

weak expression: see section 09 10 15 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5865
Entity Detected:	Mlycd, malonyl-CoA decarboxylase ( MGI:1928485)
Sequence:	sense strand is shown >T5865 GCAGAGGCCCGCTCTCCAGTGGCGTCTCCCGCTAGCAGCTGTCGCGGAGCTGTCATGCGAGGCCTCGGGC CAGGCTTGAGGGCTCGGCGCCTGCTCCCACTGCGGTCCCCGCCGCGGCCTCCGGGGCCCCGGGGACGTCG GCTGTGCGGCGGGCTCGCGGCCAGCGCCATGGACGAGCTGCTGCGGCGAGCCGTGCCCCCGACGCCGGCC TACGAGCTGCGCGAGAAGACGCCGGCGCCCGCCGAGGGCCAGTGCGCCGACTTCGTGAGCTTCTACGGCG GCCTGGCCGAGGCGTCCCAGCGCGCCGAGCTGCTCGGCCGCCTGGCTCAGGGCTTCGGCGTGGACCACGG CCAGGTGGCCGAGCAGAGCGCCGGGGTGCTGCAGCTGCGCCAGCAGGCGCGCGAGGCGGCCGTGCTGCTG CAGGCGGAGGACCGGTTGCGCTACGCGCTCGTGCCGCGGTATCGCGGCCTCTTCCATCACATCAGCAAGC TGGACGGCGGCGTGCGCTTCTTGGTGCAGCTGCGGGCCGATCTGCTGGAGGCGCAGGCCCTCAAGCTGGT GGAGGGGCCGCACGTCCGGGAAATGAACGGAGTGCTCAAAAGCATGCTGTCTGAGTGGTTCTCCTCCGGC TTCCTGAACCTGGAGCGGGTCACCTGGCACTCACCCTGTGAGGTACTTCAGAAGATCAGCGAGTGTGAGG CTGTGCACCCTGTGAAAAACTGGATGGACATGAAGCGGCGTGTGGGGCCCTACCGGAGATGTTACTTCTT CTCCCACTGCTCCACCCCCGGGGAACCCCTGGTGGTTCTGCATGTGGCTCTGACTGGTGACATTTCCAAC AACATCCAGGGCATTGTGAAGGAGTGCCCTCCGACTGAAACCGAGGAGAGGAACAGGATCGCCGCTGCCA TCTTCTACTCCATCAGCCTGACCCAGCAGGGCCTGCAGGGGGTGGAGCTCGGGACCTTCCTCATAAAGAG AGTGGTCAAGGAGCTGCAGAAGGAATTTCCTCAGCTGGGGGCCTTTTCAAGCCTGTCGCCTATCCCTGGA TTCACCAAGTGGCTGCTGGGCCTCCTGAATGTGCAGGGGAAGGAGCATGGGAGGAACGAACTATTCACAG ACTCAGAGTGCCAAGAAATCTCAGCGGTTACCGGCAACCCTGTTCACGAGAGCCTCAAGGGGTTCCTGAG CAGTGGTGAGTGGGTGAAGTCAGAGAAGCTGACGCAGGCGCTGCAGGGGCCACTAATGAGGCTGTGTGCC TGGTACCTGTACGGTGAGAAGCACCGAGGCTACGCCCTCAACCCAGTGGCCAACTTCCATCTGCAGAATG GGGCTGTGATGTGGCGTATCAACTGGATGGCTGACAGCAGCCTCAAAGGCCTCACCAGCTCATGCGGCCT CATGGTCAACTACCGCTACTACCTGGAGGAGACAGGCCCCAACAGTATCTCCTACCTGGGCTCCAAGAAC ATCAAAGCTTCCGAGCAGATCCTCAGCCTGGTAGCCCAGTTCCAGAACAACAGCAAACTCTAGGGACAGC CTTCCCAAAGCTTGGGCCCCTTCTTAGAAAGGAGGCTGTGTTCTGATGGGCCAGGCTTACTTACCACAGA ATCCTTTTCAGGGAGGCCACAGCGGTGCTGTCCTCTGACCTCAGCAGGCACTCAGGAGGCCACACCTTGG CTGTCACTGTGGTGGCCCACTCACTGCTAGGAGGCGGTGGCGGACTGGGTGCTGGGGTTCCGGGGATCCC ACTGTGCTGCTCCCCCACAGCCTGTGCCTTCAGAAAGCTCTACAGATGCCACCACAGGCCACCCAGGACA CAGCCCAGAGCACAGACCTTTTGGAGTATGAAGAGCTGACAGTCATGTTCTCTCTATTTGGGAGAGGACT AAGATCTGTACTGTGGCTAATACCGCAGCTTAAAGCTCAGACTAGAGAGAGTTTGTGCCGTGCACAGGGT GTGTTTGGCACCTCAGGGATGCTGCTGCTGCTTGAGGCCAGAGTTGGCTTACAGGGTTTAAATGAGATCC CAGTTCATAATGAACTGAACGGTAAAGAACAGATTTGTTCTGTAAGCATTAAACATGATTGTTCACTGTA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3155699 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3155699 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7229 same embryo
	EMAGE:7230 same embryo
	EMAGE:7228 same embryo
	EMAGE:7225 same embryo
	EMAGE:7227 same embryo
	EurExpress:euxassay_002721 same experiment
	MGI:4826397 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source