EMAGE mouse gene expression spatial database

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EMAGE:7242

Tbc1d13 TBC1 domain family, member 13 ( MGI:2385326)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004346. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004346_01	euxassay_004346_02	euxassay_004346_03	euxassay_004346_04

euxassay_004346_05	euxassay_004346_06	euxassay_004346_07	euxassay_004346_08	euxassay_004346_09

euxassay_004346_10	euxassay_004346_11	euxassay_004346_12	euxassay_004346_13	euxassay_004346_14

euxassay_004346_15	euxassay_004346_16	euxassay_004346_17	euxassay_004346_18	euxassay_004346_19

euxassay_004346_20	euxassay_004346_21	euxassay_004346_22	euxassay_004346_23	euxassay_004346_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7242_wholemount_strong.wlz

7242_wholemount_moderate.wlz

7242_wholemount_weak.wlz

7242_wholemount_possible.wlz

7242_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7242_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5105
Entity Detected:	Tbc1d13, TBC1 domain family, member 13 ( MGI:2385326)
Sequence:	sense strand is shown >T5105 CCACGCGTCCGCCCACGCGTCCGCGGGAGTCCGGAAGTCAACACCATGTCGAGTCTGCACAAGAGCCGGA TTGCAGATTTCCAGGATGTCCTGAAGGAGCCCTCCATTGTGTTGGAAAAGCTTCGAGAACTCAGTTTTAG TGGCATCCCCTGTGAGGGCGGACTGCGGTGCCTCTGCTGGAAGATCCTCCTGAACTACCTCCCTCTGGAG AGAGCCTCGTGGACTTCCATCCTGGCCAAGCAGAGGGGGCTCTATTCGCAGTTCCTGAGAGAGATGATTA TCCAGCCTGGCATTGCCAAGGCCAACATGGGTGTATTCAGGGAGGATGTGACCTTTGAGGACCATCCTCT GAACCCAAACCCTGACAGCAGGTGGAACACATATTTTAAGGACAACGAGGTGCTACTCCAGATTGACAAA GATGTCAGGAGGTTGTGCCCAGATATATCTTTCTTCCAAAGAGCCACTGAGTACCCCTGCCTGCTCATCC TGGACCCGCAAAATGAGTTTGAGACCCTTCGTAAGCGGGTGGAGCAGACGACACTGAAGTCTCAGACGGT GGCCAGGAATCGAAGCGGGGTTACAAATATGAGCTCCCCGCACAAGAACAGTGCGCCATCAGCCCTGAAC GAGTATGAGGTGCTACCCAATGGCTGCGAAGCCCACTGGGAGGTGGTGGAGCGCATCCTGTTCATCTATG CCAAGCTCAACCCTGGCATCGCTTACGTGCAGGGCATGAATGAGATCGTGGGCCCCCTCTACTACACTTT TGCCACTGACCCTAACAGCGAGTGGAAAGAGCACGCTGAAGCAGATACCTTTTTCTGCTTCACCAACCTC ATGGCTGAGATCCGGGACAACTTCATCAAGAGCCTGGATGACTCCCAGTGCGGTATCACCTACAAGATGG AAAAGGTGTACTCCACCTTGAAGGATAAGGATGTAGAGCTCTACCTGAAGCTGCAAGAGCAGAGTATCAA GCCTCAGTTCTTTGCCTTCCGATGGCTGACATTGCTGCTGTCCCAGGAGTTCTTGCTGCCTGATGTCATC CGTATCTGGGACTCCCTCTTTGCTGATGGCAACCGATTCGATTTCCTCCTCCTGGTCTGCTGTGCTATGC TCATACTGATCCGGGAGCAGTTACTGGAGGGGGACTTCACTGTCAACATGCGGCTTCTGCAGGATTACCC CATCACAGACGTCTGCCAGATCCTACAGAAAGCCAAGGAACTCCAAGACTCGAAGTAACGTGGTAGCAGG GTCCCAGCTTTGGAAGAGCAGCCACTGCGCTTGCTCCACACCCTTGTTTTGGGGACACAAAGGAGCCTGT GAGAAAGCCAGCCTGAAGGGAAGCTACAGTCCTGGCCAGCTCCAGGTCTTCCCAGCCTGGTTCCCATGGT CCAGGGTGCTCCTGGAGCACGCCGCGATGCGCTCCTTCCCAGTGCTCAGCCCTGCCACACTCCCTGCACC TCAGCCCTGGGACCCTCCACCCAGACTGCTCAGCAGGGCCGGAGCTCCTGAACTGGCTTCCTGGGGCTGG GGCCATTTGGGGCCTAGGAGGCTGACAGGGTCCTGCCATGCCTCTGCCCAGTAGCTCCTCACCCCTGTGC TTTCATCTTCCCTGAGTCCTGGTCAGGGGAGGCAATTAGCTAATGGGCCAGAAACAACTTCTGAGTGTTG GTGCTCAGACTGCCTGGGGAAGCACCCCAGAAGAGCTGAGTGACTGCCAGTTCTAGGGCCACCGTTTGTC CCTGTCCGGTGTGTTTGCTTGTGGCCGGTAGGGTTGTCTCCCAGGTCTCTGCTTTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTGTATGTGTGTGTGTATGTATGTATACATGTGTGTTCACACATGCCCTGGCA GTGGGGTGAGGGTATGTGGGAACCCTCTAAGGCCCTGCTTCCCCTCTGTGTCTACCTCTGCCTGTCCCTT TTCTTCCTTTGCCAGAGGCGTGTGTGGCTCCTGCTGTCTTTCTTTTGTCTGTCCTCTTTACCCTGGCGCT CTCCTCCCCTCTGCTCCCTGAGGCCAGCATTCAGGAGTGTAGCTTAGTGAGAAAGCCCTGGAGGCCAACT TTATTGGCATCCTGTTCCCATCCCCAGCCTGAGGCCTCCAGGTCCTCTATGTCAGAGCAGGAATCCCCAG TACCTGCTCCAATGGCAGCCTGTGATGCTTTGAGCCTCTCGCCCATTTTCTACTCAAGAATGGAGAAAGG AGGGATGGGGAGAGCCTTCAGTTGGAGAGTAAGATACCCTGGCTGCTGTGTGCCTGGGAGTTGCCATTTC AGGAGTGATGTCATCTCTTTGATGGCCATGTTCCCATCTGGTGTGTGTAGTTCTCATCAAGGGCAAGGGA CCAAAGAAAGCTTCTCTGGGTCCAGGGTGGCTAAACTGGAAGGCCTGGAGCTTCACAGTACCAGAGGGCT ACCTCTGCCATCTGCCCCCATGGGAGGCGGAGCGGGGAGGCGGCGGGGGGGGGGGGGGGGGGGGGAGGCT TCGGAGGTGCTGGCCTTCCTGGTCATGTGCTTGATCTATCTTCTGACTTTGGGGCTGGGTTCCCTGAACC CTATAGACTTTTCTGACCATGTTTCTTCCGGAGGTCAGGTGGCTTGAGTCCTGAAGGCAGGTTGGCAGCA AGGGCTCTTAACCTTCTCCAGAGACTGGCCAGCAGGAGTGAGGCTCCAGTAGCTGGCACCTGAAGCCTTC CTGAGAGCAGAGGGCAGGGCTGCCTCCTTCCCAGGCCATTGCTTTAGCCAGGTTGGGTTGAGTGCTGCTG GGACTCTACCTGCCTTTTTACTACCTTCTCTCTCAATACACAACACCTGGGACGTATTTTACTATTTATT TGGACACAGGCTAGCTATTTATTGCAGAGTGGCAAGTGACCTGGGGAGGAGTGGCCAGGATGCTAAAGGA GTTGGAGAATTCAAATATGGATTGTCTTATTCCCCAAGGTTAACCCTGACATCCTCTCCCCGTTCCTGCC TGAGAGGGCTGCCAGCTTTACCCTAGAGCCGAGTATAAGGGAGGCCATATACACTCCCGCCATCTTGACC GCCCCCAGACCTAGGACTTGGTAGCTGGTAGCTCAGCCCCAGTACTGCTCCCTTTGTTGACTGGGACGCA GCAAATGCTGCCCAGGACGCGGATGCATTCATTCCTTCTTGCCTGCCTCCCCCACCCCACCCCTTGCCCC GTTTCCTGGGACCATGCTACTGGAATCACTGCATCTGATTTGTTTCTGTGGGAGACCTGCATCCTGTGCC CACCACTTGGTCCTTGAGATGAAGGCCAAGGACATGGAGACTTTGTCTTGGGTAGGGTGGCTCCGCAGTT GGACGCTGCCTGAGGGAAGGATCTGCCCTTGATTCTTAAGGGGGAACCCCGAGCCTGAGCTCCTGCAGCT CCCGGGAGCTCAGCAGGCCAGGGCTGTTGATGAGTCGTCCTTAATAAACTGTGCTCTTTACCATGCAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5324875 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5324875 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7244 same embryo
	EMAGE:7243 same embryo
	EMAGE:7247 same embryo
	EMAGE:7246 same embryo
	EMAGE:7245 same embryo
	EurExpress:euxassay_004346 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source