EMAGE mouse gene expression spatial database

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EMAGE:7245

Adam8 a disintegrin and metallopeptidase domain 8 ( MGI:107825)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005205. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005205_01	euxassay_005205_02	euxassay_005205_03	euxassay_005205_04

euxassay_005205_05	euxassay_005205_06	euxassay_005205_07	euxassay_005205_08	euxassay_005205_09

euxassay_005205_10	euxassay_005205_11	euxassay_005205_12	euxassay_005205_13	euxassay_005205_14

euxassay_005205_15	euxassay_005205_16	euxassay_005205_17	euxassay_005205_18	euxassay_005205_19

euxassay_005205_20	euxassay_005205_21	euxassay_005205_22	euxassay_005205_23	euxassay_005205_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7245_wholemount_strong.wlz

7245_wholemount_moderate.wlz

7245_wholemount_weak.wlz

7245_wholemount_possible.wlz

7245_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7245_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5106
Entity Detected:	Adam8, a disintegrin and metallopeptidase domain 8 ( MGI:107825)
Sequence:	sense strand is shown >T5106 CCACGCGTCCGCCAGGCCAGTGATGAAGAGAGTACTTTGTGGAACCTCTAGGGTTAGTGTGCTCCAGATC CCATCATGCTTGGCCTCTGGCTGCTCAGCGTCTTATGGACACCAGTAGCCCCTGGACCTCCTTTGCCCCA TGTGAAACAGTATGAAGTGGTTTGGCCTCGGCGCCTAGCTGCATCCCGCTCCCGCAGAGCCCTGCCCTCC CACTGGGGCCAGTACCCAGAGAGTCTGAGCTATGCTCTTGGGACCAGCGGGCACGTTTTCACCCTGCACC TTCGAAAGAACAGGGACCTGCTGGGCTCAAGCTACACAGAGACCTACTCAGCTGCCAATGGCTCTGAGGT GACAGAGCAACTGCAGGAGCAGGACCATTGCCTCTACCAAGGCCATGTGGAAGGGTACGAGGGCTCAGCT GCCAGTATTAGCACCTGTGCTGGCCTCAGGGGCTTTTTCCGAGTTGGGTCCACTGTCCACTTGATTGAGC CTCTGGATGCTGATGAAGAGGGGCAACATGCGATGTATCAGGCAAAGCATCTGCAACAGAAGGCTGGGAC CTGTGGGGTCAAAGATACCAACCTGAATGACCTAGGGCCTCGGGCATTAGAAATATACAGGGCTCAGCCA CGGAACTGGCTGATACCCAGAGAAACCCGCTATGTGGAGTTGTATGTGGTTGCAGACAGCCAAGAGTTCC AGAAGTTGGGGAGCAGAGAGGCCGTGCGCCAGCGAGTGCTGGAGGTTGTAAACCACGTGGACAAGCTTTA TCAGGAACTCAGTTTCCGAGTTGTCCTGGTGGGCCTGGAGATCTGGAACAAGGACAAATTCTACATCAGC CGCTATGCCAACGTGACACTGGAGAACTTCTTGTCCTGGAGGGAACAGAACTTGCAAGGGCAGCACCCAC ATGACAACGTGCAACTTATCACGGGGGTTGATTTCATTGGGAGCACTGTTGGACTGGCTAAGGTGTCTGC CCTGTGTTCCCGTCACTCCGGAGCTGTGAATCAGGACCACTCCAAGAACTCCATTGGTGTAGCCTCCACC ATGGCCCATGAGCTGGGCCACAACCTGGGCATGAGCCATGATGAGGACATTCCAGGATGCTACTGTCCTG AACCACGGGAGGGTGGTGGCTGCATCATGACCGAAAGCATCGGCTCCAAGTTCCCCAGGATATTCAGCAG GTGTAGCAAGATTGACCTAGAGTCATTCGTGACAAAACCCCAGACAGGCTGCCTGACCAATGTTCCAGAT GTCAACCGGTTCGTGGGTGGCCCTGTGTGTGGAAACCTGTTTGTGGAGCATGGAGAGCAGTGTGACTGTG GCACACCTCAGGACTGTCAAAACCCCTGCTGCAATGCCACCACTTGCCAGCTGGTCAAGGGGGCAGAGTG TGCCAGTGGTACCTGTTGTCATGAATGCAAGGTGAAGCCAGCTGGAGAGGTGTGTCGTCTCAGTAAGGAC AAATGTGACCTGGAGGAGTTCTGTGATGGCCGGAAGCCAACATGTCCCGAAGATGCCTTCCAACAGAATG GCACTCCCTGCCCAGGGGGCTACTGCTTTGATGGGAGCTGTCCCACCCTGGCACAGCAGTGCCGGGATCT GTGGGGGCCAGGTGCTCGGGTAGCAGCCGACTCCTGCTATACCTTTAGCATCCCTCCGGGCTGCAATGGG AGGATGTACTCTGGCAGGATCAACCGGTGTGGAGCGCTGTACTGTGAGGGAGGCCAGAAGCCCCTTGAAC GCTCCTTCTGCACTTTCTCCTCCAACCATGGAGTCTGCCATGCTCTTGGCACAGGCAGCAACATTGACAC CTTTGAGCTGGTATTGCAGGGCACCAAGTGCGAGGAGGGAAAGGTTTGCATGGATGGAAGCTGCCAGGAC CTCCGTGTATACAGATCTGAAAACTGCTCTGCTAAATGCAACAACCATGGGGTATGCAACCACAAGAGGG AGTGCCACTGTCACAAGGGCTGGGCACCACCCAACTGTGTACAGCGGCTGGCAGATGTATCAGATGAACA AGCAGCGTCTACGAGCCTCCCAGTCAGTGTGGTTGTGGTCTTGGTGATCCTGGTGGCTGCGATGGTCATC GTGGCAGGCATCGTCATCTACCGAAAGGCTCCGAGACAAATCCAGAGGAGGAGTGTGGCACCCAAGCCTA TCTCGGGGCTCTCCAACCCCCTATTCTACACAAGGGACAGCAGCCTGCCAGCTAAGAACAGGCCTCCAGA CCCTTCTGAGACAGTTTCTACCAACCAGCCCCCAAGACCCATAGTGAAACCAAAGAGGCCTCCCCCTGCA CCTCCAGGTGCTGTGTCCAGTTCACCACTCCCAGTTCCTGTTTATGCCCCAAAGATACCAAATCAGTTTA GACCTGATCCTCCCACCAAGCCCCTCCCAGAGCTGAAACCCAAGCAGGTCAAGCCAACCTTTGCACCCCC GACACCACCAGTCAAGCCCGGGACTGGAGGGACGGTGCCTGGAGCAACTCAGGGAGCTGGTGGGCCAAAG GTTGCTCTGAAGGTCCCCATCCAGAAGAGGTGACCAGCTAGGGCACCCCAGGGCCATCGTTTGTGGACGT TTGGAGATACCACTGCTCCTATAAATGTGTTCCTTCAGCAACACCACAACCACCACCCAGGCTGGACCTG CACACGCTGTTCCTACCTCAGGAATAATCTTTCTGTGGCTCTTGCTGTCCAGATGCCCTCATGAACCAGT GGCCTCTCACTGGCCTTGGCCAGCTTAGACCATACATCTGCGTATGCTAGTTGCTAGGCAGGGGCGGTGT ACCCTGCAGTGGGCGTGATCAAGTCTGTCTGTCTCTGGGTGGACAGAATGCCAGGAGTCCCCTCCCAGCA ACTTCTGCACTGGCCCTCACACAGTGACCTCTCTCCAAGGCACAGTTCCTGTATCTACATCCCACTCGTG TATGAGACTTAGGAAAGGAAAAGACGTCGTTCTTGGGGTGTTGGGACAAGAAGTTGCCGTCATCTCCGGA TTGAAACAGATTAGTTGTTGAAATTATGACTAAACACTATTCACTAATAAAGATTCTATTTTATCAATAG ATTATAGATCACAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5324943 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5324943 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7244 same embryo
	EMAGE:7243 same embryo
	EMAGE:7247 same embryo
	EMAGE:7246 same embryo
	EMAGE:7242 same embryo
	EurExpress:euxassay_005205 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source