EMAGE mouse gene expression spatial database

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EMAGE:7270

Usp45 ubiquitin specific petidase 45 ( MGI:101850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004376. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004376_01	euxassay_004376_02	euxassay_004376_03	euxassay_004376_04

euxassay_004376_05	euxassay_004376_06	euxassay_004376_07	euxassay_004376_08	euxassay_004376_09

euxassay_004376_10	euxassay_004376_11	euxassay_004376_12	euxassay_004376_13	euxassay_004376_15

euxassay_004376_16	euxassay_004376_17	euxassay_004376_18	euxassay_004376_19	euxassay_004376_20

euxassay_004376_21	euxassay_004376_22	euxassay_004376_23	euxassay_004376_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7270_wholemount_strong.wlz

7270_wholemount_moderate.wlz

7270_wholemount_weak.wlz

7270_wholemount_possible.wlz

7270_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7270_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5206
Entity Detected:	Usp45, ubiquitin specific petidase 45 ( MGI:101850)
Sequence:	sense strand is shown >T5206 CCACGCGTCCGGCTGCGGCCCGGCGGGCGGGTAAATAACAGATGCGGGTAAAAGATCCATCAAAAGATCT ACCTGAAAAAGGCAAAAGAAATAAAAGACCACTGCTGCCTCATGATGAAGATTCTTCCGATGACATTGCT GTAGGTCTAACGTGCCAGCACGTCAGTTATGCTGTCAGTGTGAATCATGTGAAGAAAGCAGTAGCTGAGA GTCTGTGGTCAGTTTGTTCAGAATGTTTGAAAGAGAGAAGATTCTGCGATGGACAGCCAGTACTGCCTGC TGATGTTTGGTTGTGCCTCAAGTGTGGTTTGCAGGGATGCGGTAAAAACTCAGAAAGCCAGCATTCACTG AGACACTTCAAGAGCTCTGGGACAGAGTCCCATTGTGTTGTCATCAGCCTGAGTACATGGGTCATATGGT GTTATGAATGCAATGAAAAGTTGTCGACACATTGCAACAAGAAAGTTTTGGCTCAAATAGTTGATTTTCT CCAGAAACATGCTTTTAAAACACAAACAGGTGCATTTTCTAGAATCATAAAACTTTGTGAAGAAAAACGT GAAGCAGGTGAAATAAAGAAGGGGAAGAAAGGCTGCACTGTGCCATCTGTCAAAGGAATTACAAACTTAG GGAATACTTGCTTTTTTAATGCAGTCATTCAGAACTTGGCTCAGACTTACATTCTTTTTGAACTGATGAA CGAGATAAAAGAAGATGGCACCAAATTCAAGATTTCTCTTTCGTCAGCTCCACAGCTGGAGCCGTTAGTG GTGGAACTCTCGAGCCCAGGACCACTGACGTCAGCCTTGTTCCTGTTTCTTCACAGCATGAAGGAGGCTG AAAAAGGACCACTTTCTCCTAAAGTTCTTTTCAATCAGCTTTGTCAGAAGGCTCCTCGGTTTAAAGGTTT CCAGCAACAGGACAGCCAGGAGCTTCTGCACCATCTGTTGGATGCAGTGAGGACAGAGGAGACCAAGAGG ATACAAGCCAGCATTCTAAAAGCCTTCAACAACCCAACTACCAAGACTGCTGATGATGAAACTAGAAAAA AGGTCAAAGCCTATGGAAAGGAAGGTGTGAAGATGAACTTCATAGATCGCATCTTTATTGGCGAATTAAC TAGCACAGTCATGTGTGAAGAATGTGCGAACATCTCTACCATGAAGGATCCCTTCATTGATATCTCACTT CCTATCATAGAAGAAAGGGTTTCAAAACCTGTACTTTTGGGGAAAATGAGTAAATGCAGAAGTTTACAAG AAACAGACCAAGATCATAACAAGGGCACCGTGACTGTAGGAAATGCCCATCAACCCAGAGCTTCCAGGAA GCACTCCTCACCTAACGACAAGAACCAACTAAGTCATGACAGAAAACATTTAAGAAAATGGCCATCTGAA GAAGAGAAAACTGTTGTTACACACCCAAAGAATGACAACTTGGAAGCCAGCCCGCCTGCCAGCACCCTCA GCACTGAGGCGTCCCTGAACGAGAGCCTCACTGACGGCAGCGAGAGGGACGCCAGCCTAGAGAGCAGCGT GGATGCCGACAGCGAGGCTTCGGAGCCCGAGATCGCTTCCAAGCAGCCTGTGCTGCTGCGGTCCAGGGGT GATTCCTGCGGACACGCGGAGCAGCACCCACACCTCCCACTGGCAAGTGAATTGCCACAAGCCAAGGAGA CGCATGGTGGCGAGGAGGAAATGGCGGAAGCGATTGCTGAACTTCATTTGAGTGGCACTGTAACTGGCAA TAGAGATTTCCACAGGGAAAAACAGCCACTAAATGTCCCAAATAATTTGTGTTTTTCAGAGGGAAAACAT ACGAGGCTTCACAGTGCCCAAAATGCTTTTCAGACCCTTTCTCAGAGTTATGTAACTACTTCTAAAGAAT GCTCAGTCCAGTCCTGTCTCTACCAGTTCACATCCATGGAGTTATTAATGGGCAACAATAAGCTGCTCTG TGAGGACTGTACTGAAAAGAGACGGAAGTGCCACAAAGAAACCAGCTCCGCAGAAAAGAAAGCAGGAGGA GTTTATACCAATGCCAGGAAGCAACTCCTCATATCTGCCGTTCCTGCTATCCTAATCCTTCATCTTAAAA GATTCCACCAGGCTGGCTTGAGTCTTCGTAAAGTAAACAGACATGTAGACTTTCCGCTTACCCTTGACTT GGCACCGTTCTGTGCGGCTACCTGTAAGAATATAAGTGTGGGGGAGAAGGTTCTCTACGGTCTCTACGGT ATAGTGGAGCACAGCGGCTCCATGAGAGGAGGCCACTACACTGCTTACGTGAAAGTGAGAGTACCCTCCA GGAAGCTGTCCGAGTGCATCACCGGGAGGAAGACTGCAGCCGGTTTGAAAGAACCTGATGGTGAATTGGG AGGCCACTGGGTCCATGTCAGCGACACCTATGTGCAGGTGGTTCCAGAGTCAAGAGCCCTGAGTGCACAA GCTTATCTTCTTTTTTATGAAAGAATATTATAATATCTGTTCATTGTTATTTAGTTCATTTTTATTTTAA TGCTGAAGTCGTAACCATAATATGTAATGTGCCTTCTTAGTCTTTAGGAATAGTGCATCCTTCCAGCGTT ATTTAACTCTTTCAACATGTGGTGAATCCTTTAAAAAAAAATTAATTAATTTGACAGGCAGTTGTGCATG CCTTTAATTCCAGCACTTGGGAGGCAGAGGCAGGTGGATTTGAGTTCAAGGACACCCTGGTCCACAGAGC AGGTTCCAGGAAAGCCAAGACAACACAGAGAAACCTTGCCTGAAAAACCTTGGGGAAAAAAAAAACACCC TAAAATCTATATGATTTTATACATTATTCTAGTAGTGATGTACAAATAAAATGTTCATTTATTACCTAGT TCTGAGGAGATTCTGGAATGTATTTTTTTTTAATGACCTAAAGTTTGAGTGTTTTTCTTGTCACCTGAAA ATGTAGGAAAATGGTTTGATTTGATATCTAGGAATTTCTTATCAAGCTTATGTAATATAGAAAAATCACC CAGATCTACACTTAAATTTTTGTTCTTACTTTGAAGTTTTCCAAAGCACATTTGTAGAAATTTTAAATTG TGAGGCCACTGTACTAACAGATTACCTCAGGAAACCGTGCAAACAAACAAGAATGTTTACAGATCCGCAC AACTAGTCAGTGCTTTGGCACCGTGAATTTGAAAGGTAAAAGAGAGAAGTTTTTATCTAGAAACTGGTTT ACAGGGTACCTCTAGCCCCAGTTCTCCCCTGACTTTCCCATATTTAAAATAATAAAATTCTGATGATGTG CCAATTATGTGAATGCTTGTGACATAAACAAATATACATCTGTATTTAAGATTTGTTGTTTAATGTTTTA AAAAATAATATGAAGACTGCTTATGTAGGTGGTCAAGGGGATGATCATATATCAACAGAGCTATTTTACC ATAGTTCTTAGCTTGCATGTGTGATTAATTAGTGATTTTTATGTAATACTTGAATAAGATATCCTGAAGA TATGAAATGTCTTCGTGAAATGTAGCTGTGAAATGTCACCGCACATCAAATTTTTATAGTACAAAATAAC ATCCTGCCTACTTTCATGAACTTTATACTCAGCTTATTTTTCTGCATGACATCTGGTCATATAAACCTAA ACCAAATATGTGCGATAAAATCAAGCTTTCAGCTAGCATCCATTGCAGTATTAGAGAAATGATTTCTGCA TTAAAATGTTTTTGTTCATGCAGAAATCATCAATTGAAATGTGTTTAAAAGATGATTATTTTTAGGTATC AGTAATACATTCATGGTATCATTCCTTCTCTTTCATCCAGTTTTACAGTATTTTAAAAATATCTGTGTAA CTTATTGAGCATTATTTGATCATTGCTGTATGTAAAAGATCACAATAAATATTAGATTCTTATGTTAATT GATTAGGCCTAGAGTTTTTGCATTATACATTGTTAGCAGAATGTTAATAAACATTGTGTCATTGCCTAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5354502 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5354502 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7274 same embryo
	EMAGE:7269 same embryo
	EMAGE:7272 same embryo
	EMAGE:7273 same embryo
	EMAGE:7271 same embryo
	EurExpress:euxassay_004376 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source