EMAGE mouse gene expression spatial database

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EMAGE:7272

Eml3 echinoderm microtubule associated protein like 3 ( MGI:2387612)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004362. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004362_01	euxassay_004362_02	euxassay_004362_03	euxassay_004362_04

euxassay_004362_05	euxassay_004362_06	euxassay_004362_07	euxassay_004362_08	euxassay_004362_09

euxassay_004362_10	euxassay_004362_11	euxassay_004362_12	euxassay_004362_13	euxassay_004362_14

euxassay_004362_15	euxassay_004362_16	euxassay_004362_17	euxassay_004362_18	euxassay_004362_19

euxassay_004362_20	euxassay_004362_21	euxassay_004362_22	euxassay_004362_23	euxassay_004362_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7272_wholemount_strong.wlz

7272_wholemount_moderate.wlz

7272_wholemount_weak.wlz

7272_wholemount_possible.wlz

7272_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7272_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5210
Entity Detected:	Eml3, echinoderm microtubule associated protein like 3 ( MGI:2387612)
Sequence:	sense strand is shown >T5210 CCACGCGTCCGGTTTGTGCTGCAGCGCAGGGGCTCCGGACGGCCCGTCACCGCACCCCGGGAGCCTAACC CCGAGAGAGTAAAAGAACCGGTTCCACAGCGCCCCCCTCTAACTCCTGACCCCCACGCTCGTCAGCGCCC CCGAGGGGTGGCACGGCTCCCCTCCGCGCGCGCTCGAGTGCTTCCTTCCCCTCTCCCTAGCCATGGCCCC CGCGGCTTAGACGCCTCCGACGACGCTGCTCGGAGCAACGCGGGGGCCGGATGGACGGGGCTGCGGGGCC CGGTGAGGGCCCTGCTCATGAGACCCTGCAGACCCTGAGCCAGCGACTTCGGGTGCAGGAAGAAGAGATG GAACTGGTAAAAGCGGCTTTAGCAGAAGCCCTTCGCCTGCTGCGGCTGCATGGCTCCACCACAACCCTGC AGGGCTCTGGCATATCAGCCCCGACAAGGAACAGCAGCATCACAGTCCCTCCAGGACTGCCACCTACATG CAGCCCTTCCTTGGTGACCAGAGGCACCCAAACGGAAGAAGAATTGGAGATTGTTCCATCCTCTGGACCC CCTGGCCTGAGCAATGGTCCTCCAGCTCTTCAGGGGGGCAGTGAAGAACCTAGTGGGACCCAGTCTGAAG GAGGGTGCAGCAGCAGCAGTGGGGCAGGCTCCCCTGGCCCCCCAGGAATCCTCAGGCCGGTGCAGCCTCT TCAGCGTTCTGACACGCCCCGAAGAAATTCTTCCTCCTCCTCCTCCCCCTCAGAGCGGCCCAGGCAGAAA CTCTCCAGGAAGGCAGCTTCCTCAGCTAACCTGCTATTGCGGTCAGGGAGCACAGAGAGCCGGGGGAACA AAGACCCTCTGTCCAGTCCTGGGGGTCCCGGATCTCGGAGGAGCAATTATAACTTAGAAGGCATTTCAGT GAAGATGTTCCTTCGTGGCCGTCCCATCACCATGTACATTCCGTCTGGCATCCGAAGTCTTGAGGAGCTG CCCAGCGGCCCGCCCCCAGAGACCCTGAGCCTCGACTGGGTTTATGGGTACAGGGGTCGTGATTCTCGCT CTAATCTATTTGTGCTGCGCTCTGGGGAGGTGGTCTACTTTATTGCCTGTGTAGTGGTGCTGTACCGGCC TGGGGGAGGCCCAGGGGGTCCTGGAGGTGGCGGCCAGAGACATTACCGGGGTCACACAGACTGCGTTCGA TGCCTTGCTGTTCACCCTGATGGTGTTCGTGTAGCCTCAGGACAGACAGCTGGAGTGGACAAGGATGGAA AGCCCCTGCAGCCCGTGGTTCATATCTGGGACTCAGAGACACTCCTAAAACTGCAGGAGATTGGACTGGG TGCCTTTGAGCGAGGTGTGGGGGCCTTGGCCTTTTCAGCAGCGGATCAAGGTGCTTTTCTGTGTGTGGTG GATGATTCCAATGAGCACATGCTGTCAGTGTGGGACTGTAGCCGGGGAGTGAAGCTGGCTGAGATTAAGA GTACAAATGACTCAGTCCTCGCTGTTGGCTTCAGCCCTCGTGACAGCAGCTGTATTGTCACCAGTGGGAA ATCGCATGTCCACTTTTGGAACTGGAGCGGTGGGACAGGGGCTCCCGGGAATGGGCTCCTCGCCCGGAAG CAGGGTGTTTTTGGGAAATACAAGAAACCCAAGTTCATCCCTTGCTTTGTCTTCCTTCCTGATGGAGACA TCCTCACTGGAGATTCCGAGGGGAACATTCTTACCTGGGGTCGAAGTGTCTCAGATTCCAAGACCCCAGG CAGGGGTGGGGCCAAAGAGACCTACACGATCGTGGCCCAGGCCCATGCTCACGAGGGGTCCATCTTTGCC CTGTGTCTCCGCCGGGATGGGACAGTGCTGAGTGGTGGTGGGCGGGACCGACGACTGGTGCAGTGGGGGC CCGGGTTGGTGGCCCTCCAGGAGGCAGAGATTCCAGAACACTTTGGAGCTGTGAGGGCCATTGCTGAAGG GCTTGGCTCTGAGCTGCTGGTGGGAACCACAAAGAATGCATTACTGAGGGGAGATCTGGCTCAAGGCTTC TCCCCTGTTATCCAGGGCCACACTGATGAGCTCTGGGGACTGTGTACACATCCGTCCCAGAATCGCTTCC TCACCTGTGGCCATGACCGGCAGCTCTGCCTGTGGGATGGGGAAGGCCATGCACTGGCCTGGAGCATGGA CCTAAAGGAGACGGGTTTATGTGCTGACTTCCACCCAAGTGGGGCAGTTGTGGTTGTAGGACTGAACACA GGGAGGTGGTTGGTTTTGGACACAGAGACCAGAGAGATTGTATCCGATGTCACTGACGGCAATGAACAGC TCTCAGTTGTCCGGTACAGCCCAGATGGGTTGTACCTGGCTATTGGTTCCCATGACAACATGATCTACAT CTATAGTGTTTCCAGTTGTGGCACTAAGTCCAGCCGCTTTGGCAGATGTATGGGGCACTCCAGTTTTATC ACTCACCTTGACTGGTCCAAGGATGGGAATTTCATCATGTCCAATTCTGGGGATTATGAGATTCTTTACT GGGATGTGGCTGGAGGCTGCAAACTGCTGAGGAACCGCTATGAGAGCAGAGACCGGGAGTGGGCTACCTA TACCTGTGTGCTGGGCTTCCACGTCTATGGTGTGTGGCCTGATGGCTCTGATGGGACAGACATCAACTCC CTTTGCCGCTCCCACAATGAACGGGTGGTGGCTGTGGCAGACGACTTCTGCAAAGTACACCTCTTTCAGT ACCCATGTGCTCGAGCCAAGGCGCCAAGTCGTATGTACTCAGGCCATGGCAGCCATGTGACTAGTGTCCG GTTCACGCACGACGACTCATACCTTGTCTCCTTAGGTGGCAAGGATGCTAGCATCTTTCAGTGGCGAGTG CTGGGGGCAGGGAGCTCTGGGCCCGCTCCTGCCACACCATCTAGAACGCCCTCTCTGTCCCCTGCCTCCT CCCTGGACGTGTGATTGATGCTGGATGGTCTTGTCCCTCTGGTACTGTGGTCTGGCCCCACCCTGGCCAA AACCCCTAGGACCAAGGACAGTCTTTTCCTGTGCTGGCTTTTTCTTTGACTTCATACATTCCCAATTGCG CATTTTTCTGGAGGGGCTAACCACCCCACTGCACACAAAATAACAACAGACTTGCTGGCTGAGCCAGGCA ACCCAGCTCCGACACCCTCTGCTCGGTGAGGGGCAATAAAAAGAAAAAAGTAAAAAAAAAAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5354867 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5354867 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7274 same embryo
	EMAGE:7269 same embryo
	EMAGE:7273 same embryo
	EMAGE:7271 same embryo
	EMAGE:7270 same embryo
	EurExpress:euxassay_004362 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source