EMAGE mouse gene expression spatial database

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EMAGE:7277

Ddx54 DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 ( MGI:1919240)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004325. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004325_01	euxassay_004325_02	euxassay_004325_03	euxassay_004325_04

euxassay_004325_05	euxassay_004325_06	euxassay_004325_07	euxassay_004325_08	euxassay_004325_09

euxassay_004325_10	euxassay_004325_11	euxassay_004325_12	euxassay_004325_13	euxassay_004325_14

euxassay_004325_15	euxassay_004325_16	euxassay_004325_17	euxassay_004325_18	euxassay_004325_19

euxassay_004325_20	euxassay_004325_21	euxassay_004325_22	euxassay_004325_23	euxassay_004325_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7277_wholemount_strong.wlz

7277_wholemount_moderate.wlz

7277_wholemount_weak.wlz

7277_wholemount_possible.wlz

7277_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7277_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5143
Entity Detected:	Ddx54, DEAD (Asp-Glu-Ala-Asp) box polypeptide 54 ( MGI:1919240)
Sequence:	sense strand is shown >T5143 GCCCGCATGGCTGCTGGCAGACGTGTGGGACCTGGCCCGCCGTCGCGTCCCACCATGGCGCCGTGGAAGA AGAAGAGGCTGCGGAAACGCCGAACTGGGGCTTCCCAAGGCCGCGACAGCGACTCGGATGACGGCGAGTT CGAGATCCAGGCGGAGGATGACGCCCGGGCGAGGAAGCTGGGCCCTGGCAGAGCCTTGCCCTCATTTCCT ACCTCAGAGTGCGTATCAGATGTGGAGCCCGACACTCGGGAGATGGTGCGAGCCCAGAACAAGAAAAAGA AGAAGTCTGGAGGCTTCCAGTCCATGGGCCTGAGTTACCCTGTGTTCAAGGGGATCATGAAAAAGGGCTA CAAGGTGCCGACGCCCATCCAGAGGAAGACCATCCCCGTGATCTTGGATGGCAAGGATGTGGTGGCCATG GCCCGGACAGGCAGTGGCAAGACGGCCTGCTTCCTCCTCCCGATGTTTGAGCGGCTGAAGGCACGCAGTG CACAGACGGGGGCTCGAGCCCTCATCCTCTCACCCACCCGGGAGCTGGCCCTGCAGACCATGAAGTTCAC TAAAGAGCTAGGCAAGTTCACCGGCCTCAAGACTGCCTTGATCCTGGGTGGAGACAAAATGGAAGACCAG TTTGCAGCCCTGCACGAGAACCCTGACATAATCATTGCCACCCCTGGGCGTCTGGTGCATGTGGCTGTGG AGATGAACTTGAAGCTGCAGAGTGTGGAGTATGTGGTGTTCGATGAAGCAGACAGGCTCTTTGAAATGGG CTTTGCTGAGCAGCTACAGGAGATCATAGGCCGCCTTCCTGGGGGCCACCAGACGGTGCTGTTCTCAGCT ACACTGCCCAAGCTGCTGGTGGAATTTGCACGGGCAGGCCTCACAGAGCCCGTGCTCATCCGCCTGGACG TAGACTCCAAGCTCAATGAGCAGCTCAAGACCTCCTTCCTCCTTGTGCGCGAAGACACCAAGGCTGCTGT GCTCCTCTACCTGCTGCAGAATGTCGTTCGGCCCCAGGACCAGACTGTGGTGTTTGTAGCCACAAAGCAC CATGCGGAGTACCTCACAGAGTTGCTGATGGGCCAGGGTGTGAGTTGCGCCCACATCTATAGTGCCTTGG ACCAGACGGCCCGCAAGATCAACTTGGCCAAGTTCACACACAACAAATGTTCCACCCTCATCGTGACTGA CCTGGCCGCCCGGGGCCTGGACATCCCACTGCTGGACAACGTCATCAACTACAGCTTCCCTGCCAAGGGC AAGCTCTTCCTGCACCGAGTGGGCCGTGTGGCCCGAGCAGGCCGAAGTGGCACAGCCTATTCTTTGGTGG CCCCAGACGAGGTCCCCTACCTGCTTGACCTACACCTGTTCCTGGGCCGCTCTGTCACCCTGGCCCGTCC TTGTGAGGAGCCTTCAGTGGCAGATGCGGTTGGCAGGGACGGAGTGCTGGGTCGCGTGCCCCAGAGTGTA GTGGATGATGAGGACAGCAGCCTGCAGACTGCCATGGGGGCATCCCTGGATCTTCAGGGCCTGCACCGCG TGGCCAACAACGCTCAGCAGCAGTATGTGCGCTCACGGCCAGCGCCCTCGCCTGAGTCCATCAAGAGAGC CAAGGAGCTGGACCTGGCAGAGCTGGGCTTGCACCCACTCTTCAGCTCATGCTTTGAGGAGGGAGAGCTC CAACGCCTGAGGCTGGTGGACAGCATCAAGAACTATCGCACGCGCACAACCATCTTTGAGATCAATGCCT CCAGCAAGGACCCAAGCAGCCAAATGATGCGTGCCAAGCGGCAGAGGGACCGGAAAGCTGTTGCCAGTTT CCAGCAGCGGCGCCAGGAAAGGCAGGAAGGCCCAGCTGACCCAGCCCCCCAGAGGGAGCTGCCTCAGGAG GAGGAGGAGGAGATGGTTGAGACTGTAGAGGGTGTCTTCACAGAAGTCGTGGGCCAGAAACGGCCAAGGC CGGGACCCAGCCAAGGAGCCAAGAGGCGGAGGATGGAGACCCGTCAGCGAGACCAGGAGTTTTATGTCCC CTACCGGCCCAAGGATTTCGACAGTGAGCGGGGGCTGAGTGTCAGTGGGGCTGGAGGGGCCTTTGAGCAG CAGGTGGCTGGTGCAGTCCTGGACCTGATGGGGGATGAAGCACAGAACATGAGCCGGGGGCAGCAGCAGC TCAAGTGGGACCGGAAGAAGAAGCGGTTTGTGGGGCAGTCAGGCCAAGAAGACAAGAAAAAGATCAAGAC AGAGAGCGGCCGGTTTATCAGCAGCTCTTACAAGCGGGATCTCTACCAGAAGTGGAAGCAGAAGCAGAAA ATTGATGACCGGGACTCCGAGGAAGAAGGGCCATCCAACCAGCGAGGCCCTGGGCCCCGCAGAGGTGGAA AGCGAGGTCGTAGTCAAGGCACATCCCAGCCCCGAGCTTCCAGTGTACCCGCAGGCCGCATGCGCTCGGA ACTCAAGACCAAGGAGCAAATCCTCAAGCAGCGCCGGCAAGCTCAGAAGCAGCGCTTCCTGCAGCGAGGG GGCCTGAAGCAGCTTTCAGCACGCAACCGACGCCGAGCCCAGGAGCTGCGCCAGGGCGCCTTTGGCCGGG GTGCTCCCTCCAGGAAGGGCAAGATGAGGAAAAGGATGTGAGGAGCCAGACGCAGCCCTGGGGCTTCCTG GTAGCCCCGGGTGTGGACGTCAGGGACTATGTCCATGTGCTGTTGGAAGATCCTTCCACAGGCGCTGCTC TGTGAGGAGTAGTGCCATATGGCCACAGAGCAACAGCTGCTTTTGACTGGGACATTGGGTGACCTCTGAA AGGATGCATAGGAGTCTAGCTATGCAAAGCAGGCAGACCCAAGTCCTGACCCTGCAAGTCACAGCGGCTT CTGGTTCCACACCTTCAGGATTCAGAGATCAGGGCCGTGTGGATGCCTCTGACCCAGCATTGAGTTTTAA TGTAATAAACTTTACTGCCTCTAGTAGACCAGGAGTGTGGCTTCCTCAGGATGTTTTTGGAAGGTGTCTC AGCTGTTGTTGAGGCTGAGAGCAGGTGGCCATGCCTGCGTGTCCCTCCTGGTCACTGAGATGAGGAAAAT GAGTCACCCAAGGCAGCTCAGGACTGCTGCGGGGCAGAGAAAGGCTGTGGTGGCATTGCCCTTCTTCACA TGGAAGAGAGAAAGCAGCCTGCGCCAACCCTCGTGTCTTCATTCTGCTGGTGGCTTTTTCCACCTCTTGC TTGAGGTCCTGGGTGTGTGTGTGTGAAGCATCCTCCAGTAGCCAGACCCCAGTGCAGGTCAGGCACACAT TGGGGACTACCCCGTTGGGCCCGAAGAGCAGATGGTACCAATAATCCCCTGCCGCTCAGATCCACTGTAC ATAGCAAGTGCACATCCTGGAGTGCACTGGGCTCCATCTGAGCTTGTCCAATGCCCCCCCCCCCAGCCAG CTGGGAGAGGCCTGTCCCCAAGCCTGTTGTGGTGGATGGAGTGACCAGCCCAGCTGTCAACCCCATTGTC TACTTAACTGAGTTCATACTGAATGTCCCGTGGAAGGCTCACAATGGCGTGAGGCGTCACCGCTCTGTCT TGCCCAGGAGAAAGGATAGTCAGAGAGCGGTTACGTTGAGCCTGCACACCAAGAAATGTGGGACTTGGTC CCTCAGGTCCCCTTCACCGTTCGCCAGGCCGGATTAGGAAGCAGTAGCTGTAGGCTGAGCCAGGGCAAGT TTGGCCAGTGTGGCTGAGATGTTCATGATAAACCGCTTCACCTGGGGGCAGAGATGACATGGCCAGAGTA CATCCTCTCCCCACCCCCACAGAGGCAGGCCCAGGGCAGGGGTGGGGCTGGAACCCTGACTGGAGAGGCA CTGATAGTGTCTAGCCCAGCCTCCTAAAAGTATCCTTTGTTCTGTTTATTGATAAAAGTACAACTCAAAC TTGGCCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5344158 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5344158 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7280 same embryo
	EMAGE:7276 same embryo
	EMAGE:7279 same embryo
	EMAGE:7275 same embryo
	EMAGE:7278 same embryo
	EurExpress:euxassay_004325 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source