EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:7280

Adamts4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 ( MGI:1339949)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004320. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004320_01	euxassay_004320_02	euxassay_004320_03	euxassay_004320_04

euxassay_004320_05	euxassay_004320_06	euxassay_004320_07	euxassay_004320_08	euxassay_004320_09

euxassay_004320_10	euxassay_004320_11	euxassay_004320_12	euxassay_004320_13	euxassay_004320_14

euxassay_004320_15	euxassay_004320_16	euxassay_004320_17	euxassay_004320_18	euxassay_004320_19

euxassay_004320_20	euxassay_004320_21	euxassay_004320_22	euxassay_004320_23	euxassay_004320_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7280_wholemount_strong.wlz

7280_wholemount_moderate.wlz

7280_wholemount_weak.wlz

7280_wholemount_possible.wlz

7280_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7280_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 17 19 weak expression: see section 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5156
Entity Detected:	Adamts4, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 ( MGI:1339949)
Sequence:	sense strand is shown >T5156 CCACGCGTCCGTTTTGGTGCCGCAGATGGCCTCAATCCATCCCAGCTGCAGCCCGGGTACCATGTCCCAG ATGGGCTTGCATCCCAGGAGGGGCTTGACTGGGCACTGGCTGCGAAGATTCCAACCCTGCTTGCCGCTTC ACACTGTGCAGTGGCGGAGGCTGCTGCTGCTGGCCTTCCTCCTGTCCTTAGCGTGGCCCGCCAGCCCCCT CCCCCGGGAGGAGGAGATCGTGTTTCCAGAGAAGCTCAATGGCAGTAGCATCCTACCTGGATCAGGCGTT CCTGCCAGGCTGCTGTACCGATTGCCAGCCTTTGGGGAGATGTTGCTACTAGAACTAGAACAGGACCCTG GGGTGCAGGTAGAGGGTTTGACTGTACAGTACCTGGGCCAGGCACCTGAGATGCTGGGTGGGGCAGAGCC AGGTACCTACCTGACTGGCACCATCAATGGAGATCCGGAGTCGGTGGCATCTCTGCACTGGGACGGGGGA GCCCTATTAGGGGTACTGCAGTACCGTGGGGCCGAACTCCACCTCCAGCCTCTGGAAGGAGGCGCCCTTA ACTCTGCTGGGGGACCGGGGGCTCACATCCTACGCCGGAAGAGTCCTGCCAGCAGCCAAGGTCCCATGTG CACCGTCAAGGCTCCTTCTGGGAGCCCAAGTCCCATTTCCCGCAGAACCAAGCGCTTCGCTTCTCTGAGT AGATTCGTGGAGACACTGGTGGTAGCAGATGACAAGATGGCAGCATTCCATGGTACAGGGTTAAAGCGCT ACCTGCTGACGGTTATGGCAGCCGCCGCTAAAGCCTTTAAACACCCAAGCATCCGAAACCCTGTCAACTT GGTGGTGACGCGCCTGGTGATCCTGGGGTCCGGCCAGGAAGGGCCCCAAGTGGGGCCAAGTGCCGCCCAG ACCCTACGCAGCTTCTGCACCTGGCAGCGGGGCCTCAACACCCCTAACGACTCAGATCCTGACCACTTTG ACACAGCCATTCTGTTCACCCGGCAGGACCTGTGTGGGGTCTCCACTTGTGACACCCTGGGTATGGCTGA TGTGGGCACAGTGTGTGATCCAGCTAGGAGCTGTGCTATTGTGGAAGATGATGGGCTCCAGTCAGCCTTC ACTGCTGCTCATGAACTGGGCCATGTCTTCAACATGCTCCATGATAACTCCAAGCCATGCACTAACTTGA ATGGGCAGGGGGGTTCCTCTCGCCATGTCATGGCTCCTGTCATGGCCCATGTGGACCCTGAAGAGCCCTG GTCGCCCTGCAGTGCCCGATTCATCACTGACTTCCTGGACAATGGTTATGGGCACTGCCTCTTAGACAAA CCGGAGGCTCCCCTCCATCTACCAGCGACTTTTCCTGGCAAGGACTATGACGCTGACCGCCAATGCCAAC TGACCTTCGGTCCTGACTCAAGCCATTGTCCACAGCTGCCACCGCCCTGTGCTGCCCTCTGGTGCTCTGG CCACCTCAATGGCCATGCCATGTGCCAGACGAAGCACTCACCTTGGGCTGATGGCACTCCCTGCGGGTCT TCACAGGCCTGCATGGGTGGCCGCTGTCTGCACGTGGACCAGCTCAAGGACTTCAATGTTCCTCAGGCTG GCGGCTGGGGCCCCTGGGGACCATGGGGTGACTGCTCCAGGACTTGTGGGGGTGGTGTCCAGTTCTCCTC CCGGGATTGCACGAGGCCCGTCCCCCGGAACGGTGGCAAGTATTGTGAGGGCCGCCGGACTCGCTTCCGG TCCTGCAACACGGAGAACTGCCCACACGGCTCAGCATTGACCTTCCGTGAAGAGCAGTGTGCTGCCTACA ACCACCGAACCGACCTCTTCAAGAGCTTTCCAGGGCCCATGGACTGGGTTCCGCGCTACACAGGTGTGGC CCCTCGAGACCAATGCAAACTCACTTGCCAGGCCCGGGCACTGGGCTACTACTACGTACTGGAGCCCCGG GTGGCAGATGGGACTCCCTGCTCCCCAGACACCTCCTCTGTCTGTGTCCAGGGCCGCTGTATCCATGCTG GCTGTGACCGGATCATTGGCTCCAAAAAGAAATTTGACAAGTGCATGGTGTGCGGCGGGGATGGCTCTCG CTGCAGCAAGCAGTCGGGCTCCTTCAAAAAATTCAGGTATGGATACAGCGATGTGGTCACGATCCCTGCG GGGGCCACCCATATCCTTGTACGGCAGCAGGGGGGGTCTGGTCTCAAGAGCATCTACCTGGCCCTGAAGC TTTCTGACGGTTCTTACGCCCTCAATGGTGAATACACGCTGATGCCCTCCCCAACAGATGTGGTTCTTCC TGGGGCAGTCAGCTTGCGCTACAGCGGAGCCACAGCAGCCTCAGAGACACTGTCTGGACATGGGCCGCTG GCCCAGCCCTTGACGCTGCAAGTCCTGGTGGCTGGCAACCCACAGAATGCACGTCTGCGGTACAGTTTCT TTGTCCCGCGGCCAGTCCCTTCAACACCACGCCCTCCTCCCCAAGACTGGCTGCAACGCAGGGCAGAGAT ACTGAAAATCCTTCGGAAGCGTCCCTGGGCAGGCCGGAAATAACCTCACTGTCCCGGCTGCCCTTTTTGG GCGCCGGGGCCTCGGACTCATCTGGGAGAATGAGCAGGCTTCTGCAACTGCCTCCTGCTAAAACACAGTA GGGAGGTGTAGAGGGTGAGATCTGCCTGCCTCACTGCCCCAAACCGCAGGCTGGCCCTGCCCTGGCTTCC TGCCCTGGGAGGCAGTGATGTCTTGGTGAATGGAAAGGGGCTAGGTGACAGTACCCTATCTACTAAACTG CCCCCTCTACCCTGCAGGTCACAGGAGGAATGGGGGAAAGACAGGGTGGGTCCTGGGCCCTAGTTGTATT TATTTGGTATTTATTCATTTTTATTTAGCACCAGGAAAGGGGACTAGGGTCTTGGGGAAACTCACCCATT ATAGCCCTAACCTAGCTATGAAATCCAGGGTGTTGGTGACAAATATGAGTGGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTGTGTGTGTTTATGTATGAGGTACAACCTGCCCTGCTTTCCTCTCCCCTAATTTTTTTTT TTTTTTCTGGGAAAAGGAAAGTCAAAGGTAGGACTGCCTTCAGGGAGTAAGGGATGATTGTGTTTTTAAA TTGAAGTTTGCTATTTATATGCTCTTTTTGGAGTCAGACAAATGTGGGTTATATTCTGGCCCCGCATCTT TGAGCATTAGTTTTCTCATGTGCCAATAATAATCCCTTAGAAATTGGTTGTAAGGATTAAATGATGTAAA TAAAGAACTAGCATAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5345809 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5345809 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7277 same embryo
	EMAGE:7276 same embryo
	EMAGE:7279 same embryo
	EMAGE:7275 same embryo
	EMAGE:7278 same embryo
	EurExpress:euxassay_004320 same experiment
	MGI:4822989 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source