EMAGE mouse gene expression spatial database

EMAGE:7292

Gmfg glia maturation factor, gamma ( MGI:1927135)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004389. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004389_01	euxassay_004389_02	euxassay_004389_03	euxassay_004389_04

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euxassay_004389_10	euxassay_004389_11	euxassay_004389_12	euxassay_004389_13	euxassay_004389_14

euxassay_004389_15	euxassay_004389_16	euxassay_004389_17	euxassay_004389_18	euxassay_004389_19

euxassay_004389_20	euxassay_004389_21	euxassay_004389_22	euxassay_004389_23

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

single cell

moderate expression: see section 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6395
Entity Detected:	Gmfg, glia maturation factor, gamma ( MGI:1927135)
Sequence:	sense strand is shown >T6395 CCAACACTGAGGTCAAGACCATTGCCCCACCTCGGGCACAAATTGAGGCTGGAGACCAGGATTTAAGGAT CATTAGAGATGTCGGACTCCCTGGTGGTGTGTGAAGTGGACCCGGAGCTAAAGGAAACATTGAGGAAATT TCGTTTCCGAAAAGAGACCAACAATGCCGCCATCATAATGAAAGTGGACAAAGACCGGCAGATGGTGGTG CTGGAGGATGAACTCCAGAACATCTCCCCAGAAGAACTTAAGTTGGAATTGCCAGAGAGACAGCCCAGGT TTGTGGTCTACAGCTACAAGTACGTGCATGATGACGGCAGGGTGTCCTATCCTTTGTGCTTCATCTTCTC CAGCCCCGTGGGCTGCAAGCCTGAACAACAGATGATGTACGCAGGAAGTAAAAACAGGCTGGTGCAGACG GCGGAGCTTACCAAGGTGTTTGAAATCCGCACCACAGACGACCTCACCGAGACCTGGCTTAAGGAAAAGT TAGCTTTCTTTCGTTGACCTTTGAGCCAGGGATTTGGAATCCTTACTTCTGAAGGCTCAGATTGATAGGA TATGAACATCCATGAGCGAAATAAAGGTTAAAATTTCAGGAACTTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3581858 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3581858 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7295 same embryo
	EMAGE:7297 same embryo
	EMAGE:7296 same embryo
	EMAGE:7293 same embryo
	EMAGE:7294 same embryo
	EurExpress:euxassay_004389 same experiment
	MGI:4825123 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source