EMAGE mouse gene expression spatial database

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EMAGE:7329

Trim21 tripartite motif-containing 21 ( MGI:106657)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004419. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004419_01	euxassay_004419_02	euxassay_004419_03	euxassay_004419_04

euxassay_004419_05	euxassay_004419_06	euxassay_004419_07	euxassay_004419_08	euxassay_004419_09

euxassay_004419_10	euxassay_004419_11	euxassay_004419_12	euxassay_004419_13	euxassay_004419_14

euxassay_004419_15	euxassay_004419_16	euxassay_004419_17	euxassay_004419_18	euxassay_004419_19

euxassay_004419_20	euxassay_004419_21	euxassay_004419_22	euxassay_004419_23	euxassay_004419_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7329_wholemount_strong.wlz

7329_wholemount_moderate.wlz

7329_wholemount_weak.wlz

7329_wholemount_possible.wlz

7329_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7329_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 12 weak expression: see section 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6429
Entity Detected:	Trim21, tripartite motif-containing 21 ( MGI:106657)
Sequence:	sense strand is shown >T6429 GGAGCACATAACTAAAGCTGAGGACCCTGGTTAGAAACTCGGTTTCCCCTTCTGCCTGGCTTGCTCAGTG GGTGAGGCCGGAGGAGAGGCTCCTGTCATTCAGATTCCACAGCAGATGTCACCCTCTACAACCTCAAAAA TGTCTCTGGAAAAGATGTGGGAGGAGGTCACCTGTTCTATCTGCCTGGATCCCATGGTGGAGCCTATGAG TATCGAATGTGGCCATTGCTTTTGCAAGGAATGCATTTTTGAAGTTGGGAAGAATGGGGGCAGTTCATGT CCCGAGTGCCGGCAACAGTTTCTGCTCCGAAACCTCAGGCCCAATAGACATATAGCCAACATGGTGGAAA ACCTTAAACAGATAGCCCAGAATACCAAGAAGAGTACCCAGGAAACGCACTGCATGAAGCATGGAGAGAA GCTTCACCTATTCTGTGAGGAAGATGGGCAGGCCCTTTGCTGGGTGTGTGCCCAGTCTGGGAAACACCGG GACCACACCAGGGTCCCTATTGAAGAGGCTGCTAAGGTATACCAGGAGAAGATCCACGTGGCTTTAGAAA AACTGAGAAAGGGGAAAGAGTTGGCCGAGAAGATGGAAATGGATCTCACGATGCAAAGAACAGACTGGAA GAGGAACATTGACACCCAGAAGTCGAGGATTCACGCAGAGTTCGCACTTCAGAATAGCTTGCTGGCTCAG GAGGAGCAGAGGCAGCTGCAGAGGCTGGAGAAGGATCAAAGGGAGTACCTGAGACTCCTGGGGAAGAAGG AGGCTGAGCTGGCTGAGAAGAACCAGGCCCTGCAGGAGCTGATCTCAGAGCTGGAGAGGAGGATTCGTGG TTCAGAGCTGGAGCTACTGCAGGAGGTGAGGATCATCCTGGAAAGGAGTGGATCCTGGAACCTGGACACG TTAGATATTGACGCCCCAGACCTAACAAGCACATGCCCTGTGCCAGGGCGGAAGAAGATGCTGAGGACGT GTTGGGTTCATATTACTCTGGATCGCAACACCGCCAACTCATGGCTCATCATCTCAAAGGATCGGAGACA AGTGAGGATGGGAGACACCCATCAGAACGTGTCTGACAATAAGGAGAGGTTTAGTAATTACCCCATGGTG CTAGGTGCCCAGAGATTCTCCTCTGGGAAGATGTACTGGGAGGTAGATGTGACTCAAAAGGAGGCCTGGG ATCTGGGGGTTTGCAGAGATTCTGTTCAGAGGAAAGGGCAGTTTTCACTCAGTCCCGAGAATGGCTTCTG GACCATTTGGTTATGGCAAGACAGCTATGAGGCTGGTACCAGTCCTCAGACCACCCTCCACATTCAAGTA CCTCCATGCCAAATTGGGATCTTTGTGGACTATGAGGCTGGCGTTGTCTCCTTCTACAACATAACTGACC ATGGCTCCCTCATTTACACCTTCTCGGAGTGTGTTTTTGCTGGACCTCTGCGACCTTTCTTCAATGTTGG TTTCAATTATAGTGGGGGAAATGCAGCGCCTCTAAAGCTCTGTCCACTAAAGATGTGATGGTCAGGAGCC AGTGCCTACTGACGGTACTTCCCGGACACTTACCTCCTCCTGTCCTGATCAAGATCCAGTGACTCCTGGG GACCATTTCTGGATACTGCTGCTCACTTCCTGTTGGTGTTCTTCAGCCATGCACTTTGTCATTTTGACCC ATCTAGTTGGAACTACCCTAAACCCTCATCTCTTTAAAGGAGTCAGGGTCCCAGAATGAAGGCATCAGCT GGACATAACTGAAAATCAATGTCAAATGACCTTCACCATCAATATACCTGACATAGATTCCGCAATGATT CCTCAATGAAACAGACTTCCTCACATTGGACCCAAACTCATCTAACACAGACAAACAAACATTTCTGCCT TTATGTGACTTCCTTTGTTTTCTCTGTCCTTGGTTGTTACCTTTGGCTAGATACCTGTCTACTCCTATAT CTTTTGTGGCTTACAGAAGGACTTCCTAGAAATTGTGCTTGAACTATCCTGTTCAGTTATTATTGGTAAA ATATATATATATATATGTCTTAACATTATACATCTTCTGGTTTGGTTTGTTGTTTTGTTGTTACTGGGGG ATTTTAGGGTTTTATTGTTCGTTGTTTGTTTGTTTTCCCTTCCTGGAATGCTCTATGCACTTCTTCTGTA AACCCAGGTCAAGAATATCTTCCATGGGAACAAACGAAAGCCAGGATAAAGTAGTGAAAGTGGTTCAGAC AAGAGCTGAATGTTACACTGACAGTATGCTCAGTATCCCCAGTGATAGCCAAAGTCTGGGTGCTGTGCTG GGTCTGGTCACTGAACCCCTGCTGTTGACTCTCACAAGGCTACAACAGCTGTGCTCAGACACTGCCTGCT GTCATTTTGTGGATATGTTGGTTTCTGGTTCCATTGCCTTGAAGAAAGTGAAATTAGTAGCTGAACTAGT ATACAAATATTTTGAGAGCTTCAAAAATACTACCTGGAGATATATGTTACATAGTTAATAAACCCCTTAT TCTCCACTGAATGGAATTGGGAGGTTTCATACTATCTCAGCTTTCCCCCATAGTCAATAACAAATGGTCT GGATACAAGTGTCTTTGTTACATAAAAGTTACCTTGAGAAAGATCTCCAACTATTGGTTCCAATTCTGAC TAATATGTCTTTTAAGATCAAATAAAGCCTGTTGATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3584654 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3584654 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7328 same embryo
	EMAGE:7331 same embryo
	EMAGE:7326 same embryo
	EMAGE:7330 same embryo
	EMAGE:7327 same embryo
	EurExpress:euxassay_004419 same experiment
	MGI:4828909 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source