EMAGE mouse gene expression spatial database

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EMAGE:7339

Llgl1 lethal giant larvae homolog 1 (Drosophila) ( MGI:102682)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005282. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005282_01	euxassay_005282_02	euxassay_005282_03	euxassay_005282_04

euxassay_005282_05	euxassay_005282_06	euxassay_005282_07	euxassay_005282_08	euxassay_005282_09

euxassay_005282_10	euxassay_005282_11	euxassay_005282_12	euxassay_005282_13	euxassay_005282_14

euxassay_005282_15	euxassay_005282_16	euxassay_005282_17	euxassay_005282_18	euxassay_005282_19

euxassay_005282_20	euxassay_005282_21	euxassay_005282_22	euxassay_005282_23	euxassay_005282_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7339_wholemount_strong.wlz

7339_wholemount_moderate.wlz

7339_wholemount_weak.wlz

7339_wholemount_possible.wlz

7339_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7339_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8931
Entity Detected:	Llgl1, lethal giant larvae homolog 1 (Drosophila) ( MGI:102682)
Sequence:	sense strand is shown >T8931 CGGAGGCGGGCGCAAGATGATGAAGTTTCGGTTCCGGCGGCAGGGCGCCGACCCGCAGCGCGAGAAGCTT AAGCAGGAGCTCTTCGCCTTCCACAAGACTGTGGAGCATGGCTTCCCCAATCAGCCCAGCGCCTTGGCCT TCGATCCTGAGCTCCGCATCATGGCCATCGGCACCAGATCTGGGGCCGTCAAGATCTATGGTGCACCCGG AGTGGAATTTACAGGCCTACATCGGGACGCAGCCACCGTCACCCAGATGCATTTCCTCCCTGGTCAGGGC CGCCTCCTGACCCTGCTAGATGACAGCAGCTTGCATCTCTGGGAGATCATCCATCATAATGGCTGCGCCC ACCTGGAGGAAGGCCTCAGCTTCCACCCACCCAGCAGACCCAGTTTTGACAATGCCAGTTTCCCTGCCAG TCTAACACGTGTCACTGTGGTCCTGCTCGTAGCTGGCAATACAGCAGCCCTGGGAACTGAGAGTGGTAGC ATATTCTTCCTGGATGTAGCCACCCTGGCACTGCTGGAGGGGCAGACTCTCAGCCCAGATGTGGTTCTGC GCAGTGTGCCAGATGATTACCGGTGTGGAAAGGCCTTGGGCCCTGTGGAGTCACTCCAGGGACATCTGCA AGACCCCAGCAAGATCCTCATAGGCTACAGTCGGGGTTTACTGGTCATCTGGAGCCAGGCCACACAGTCT GTGGACAACGTTTTCCTAGGTAACCAGCAGCTGGAGAGCCTGTGTTGGGGCCGTGATGGCAGCAGCATTA TCAGCTCACACAGTGATGGCAGCTATGCCATCTGGTCCACAGACACTGGCAGCCCCCCAACGCTGCAGCC CACTGTAGTGACCACACCCTACGGCCCCTTCCCCTGCAAGGCCATCAACAAGATTCTGTGGCGGAGCTGT GAGTCAGGAGACCACTTTATCATCTTCAGTGGTGGCATGCCTCGAGCCAGCTATGGTGACCGCCACTGTG TGAGTGTACTGCGAGCAGAGACACTGGTGACCCTGGACTTCACCTCTCGTGTCATTGACTTCTTCACGGT GCACAGCACACAGCCAGAGGATGAGTGTGACAACCCCCAGGCCTTAGCCGTGCTTCTGGAGGAGGAGCTG GTGGTGCTGGACCTGCAGACACCAGGCTGGCCAGCTGTGCCCGCCCCTTACCTGGCCCCACTGCATTCGT CAGCTATCACCTGCTCTGCCCATGTTGCCAATGTCCCCAGCAAGCTGTGGGCCCGCATTGTAAGTGCTGG TGAGCAGCAGAGCCCACAGCCTGCCTCCAGTGCCTTGAGTTGGCCCATTACCGGGGGCCGGAACTTGGCC CAGGAACCCTCGCAGCGTGGGCTGCTGCTCACTGGCCATGAGGATGGCACTGTGCGGTTCTGGGACGCCT CTGGTGTGGCGCTAAGGCCACTCTACAAACTGAGCACAGCTGGCCTCTTCCAGACGGACTGTGAACATGC TGACAGCCTGGCCCAGGCTGTGGAGGATGACTGGCCGCCCTTCCGCAAGGTGGGCTGCTTTGATCCCTAC AGTGATGACCCCCGGCTAGGAATCCAGAAGGTTGCGCTTTGCAAGTACACAGCCCAGATGGTGGTAGCTG GCACTGCAGGCCAGGTGCTGGTGCTGGAGCTCAGTGAGGTCCCAGCAGAGCATGCCGTCAGTGTGGCCAA CGTGGATCTTCTTCAGGATCGCGAGGGCTTCACGTGGAAGGGTCACGAGCGGCTGAACCCACACACGGGG CTGCTGCCGTGGCCTGCCGGATTCCAGCCCCGCATGCTGATACAGTGCCTCCCACCCGCCGCTGTCACTG CTGTCACACTCCATGCTGAGTGGAGCCTCGTGGCCTTTGGTACCAGTCATGGCTTTGGCCTTTTTGACTA CCAGCGCAAGAGCCCTGTGCTGGCTAGGTGCACCCTTCACCCCAATGATTCTTTGGCCATGGAGGGGCCA CTGTCACGGGTGAAGTCCCTCAAGAAGTCACTGAGACAGTCATTCCGGCGAATCCGCAAGAGCCGTGTCT CAGGCAAAAAACGGACTCCTGCTGCCAGTAGCAAGTTGCAGGAGGCCAATGCCCAGCTGGCCGAGCAGAC CTGCCCACACGACCTGGAGATGACACCCGTGCAGCGCCGCATTGAGCCTCGGTCTGCTGACGACTCGCTC TCCGGTGTTGTACGCTGCCTCTACTTTGCTGACACGTTCCTTCGAGATGCGACCCACCACGGGCCCACCA TGTGGGCGGGCACCAACTCGGGCTCTGTGTTCGCCTATGCGCTGGAGGTTCCAGCAGCCACAGCAGGCGG AGAGAAGCGGCCTGAGCAGGCAGTGGAGGCGGTGCTGGGCAAGGAGGTGCAGCTAATGCACCGAGCACCT GTGGTGGCCATTGCTGTGCTGGATGGTCGTGGCCGGCCACTGCCTGAGCCCTATGAGGCTTCCCGGGACC TGGCCCAGGCGCCAGACATGCAAGGCGGCCATGCTGTGCTCATTGCATCTGAGGAACAATTCAAGGTGTT CACACTACCCAAGGTGAGTGCTAAGACTAAATTCAAGCTTACAGCCCATGAAGGCTGTCGTGTGCGGAAG GTAGCCCTGGCTACATTTGCCAGCGTGATGTCTGAGGACTATGCCGAGACCTGCCTTGCCTGCCTCACCA ACCTGGGTGATGTCCACGTCTTCTCGGTGCCTGGCCTGAGGCCTCAGGTGCACTACTCCTGTATCCGGAA GGAGGACATCAGTGGCATCGCTTCCTGTGTCTTCACACGTCACGGCCAGGGCTTTTACTTGATTTCTCCA TCGGAATTTGAGCGCTTCTCACTGAGTGCTCGCAACATCACAGAACCACTATGTTCTCTGGATATAAGCT GGCCCCAAAATGCCACCCAGCCCAGGCTTCAAGAGTCACCCAAGCTGAGCCAGGCTAATGGGACCAGAGA CATCATTCTGGCCCCAGAGAGCTGCGAAGGAAGCCCTAGCTCTGCCCACAGCAAGCGAGCTGATACCATG GAACCACCCGAGGCCGCTCTCTCGCCTGTGTCCATTGATTCAGCTGCTAGTGGGGATACCATGCTGGACA CAACAGGGGATGTCACCGTGGAATATGTGAAGGATTTTCTGGGGTGAGGCCAGCTGCTCTCCCAAGCCGC CATCCCCAGGGCAACTAGGGGTCTTCTTACCCCAGCCCTTCTGATTACTGCCCTCCCCAGCTCTCCTGAG GACTCTGAGAAGAACCTGCGGAACCTGGAGGCAGATGATGCTTGTCGGGCCTACACCCTTCTGATTAAAT GAGGTGCTGTGGGGCAGCCTGGCCTTACCACACAGCAGCTGCCATCCCTCACTCCAGCTGGAACCCTGCA GGCTTCTCTAATGGTGTCATTCCTCGAAGCCTAGCTGCCTTCCTCTGGCTGTCAGGCATAGGGGATCCAC AGTCATGAAAGGGCCTGGCCTGGCAGCACTGAAATCCTGTGCATTTAGAAGGCCACCTCTGACCCTGTGG GTGTAGGAGTTGATGCTTATCCAGTCCACAGAGCCCATTTCACAGGAGAGTCACTGAGGCTGATAGGTCC TGCCTATTGAGCCAGCACATGGACCTTATGTGCCCCTACCGTATCTTGCTATGGACCCAGCCTAGCTGGA GACTGGAGCTCTCTGGACCTTAGTAGCTAGTGCCTCTGCAGCCTACATTGGGCATAGATAACTAACCATA CCCTCTTCTACTTGGTACCCTCCTAGCTCCCCAGCAAGCCCATCTTGGGCCATCACTGAAAGGGGCTGCC TACCCCGCAGGCAGGCATGACCTGGGTTTGCTCCCCTTAGTCGTCACCATCTGTCGGTCTTGCCTTGCCC CTTAACACGGACCCGCTGACTGATGCTGGGCCCTGAACACATCCCAGCCTCAAGCTCAGCCGGAGGGGTG CGAGCATCCTTTTGCACTCCATACTGTACTTCCTTTCTCCTCAAGCAGAGTATTTTTCTAACGCCTGGAC TGAGCTGGCCAGTCATTTCTAAAGCAGTTTTGGTACTTGCTCCTGGCCTGTGTGTATTGTGTGCTCCTGT GACTAGAGACTGCTGGGCGTCATGACTGAAGTCAGTCAGAGGTGTGTGTGTGCGTGTGTGTGCACGTGTG TGTGCGGCAGTGAGGATGGGCTTCCCTGTAGATGCCACCTTCGGCTTTTCTGTCTTTTTTTAAAAACTAG TTGTTTTAATATTAAAAGTACTGATTTTTTAATATTGAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6418169 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6418169 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7340 same embryo
	EMAGE:7341 same embryo
	EMAGE:7342 same embryo
	EMAGE:7343 same embryo
	EMAGE:7338 same embryo
	EurExpress:euxassay_005282 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source