EMAGE mouse gene expression spatial database

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EMAGE:7340

5730559C18Rik RIKEN cDNA 5730559C18 gene ( MGI:1921579)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005271. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005271_01	euxassay_005271_02	euxassay_005271_03	euxassay_005271_04

euxassay_005271_05	euxassay_005271_06	euxassay_005271_07	euxassay_005271_08	euxassay_005271_09

euxassay_005271_10	euxassay_005271_11	euxassay_005271_12	euxassay_005271_13	euxassay_005271_14

euxassay_005271_15	euxassay_005271_16	euxassay_005271_17	euxassay_005271_18	euxassay_005271_19

euxassay_005271_20	euxassay_005271_21	euxassay_005271_22	euxassay_005271_23	euxassay_005271_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7340_wholemount_strong.wlz

7340_wholemount_moderate.wlz

7340_wholemount_weak.wlz

7340_wholemount_possible.wlz

7340_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7340_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 17 18 19 20

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 16 17

esophagus epithelium

moderate

regional

moderate expression: see section 13

pharynx epithelium

moderate

regional

moderate expression: see section 13 14 15

stomach

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09

rectum

moderate

regional

moderate expression: see section 09 10

midgut

strong

regional

strong expression: see section 13 moderate expression: see section 08 09 10 11 12 14 15 16 17 18

metanephros

moderate

regional

moderate expression: see section 05 06 07 08 14 15 16 17

lung

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

trachea

moderate

regional

moderate expression: see section 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8706
Entity Detected:	5730559C18Rik, RIKEN cDNA 5730559C18 gene ( MGI:1921579)
Sequence:	sense strand is shown >T8706 GGGAGACAGGGAGCAGCGTCGCCCCTCGCGCTGTTGCTGCCGCAGGATGCACACTTGGGCTGGCATTGCC CCAACCCAACGTGAGGCCCCGCCCCGTCTCTGGGTTCAGAATCTCGAATCCCTTCTAGCATCTTCCCCTC TCTTCACTGGGCCTTTGGTATGCCCCAGGGGACAGATGTCTTGGGATCTTTAACATTTGGGATGCTGCAA ATGCCGAAGTTAAATGAAATACCTCCGGGGAGGGGAGGCCCGGGGGAGCCTTGGGGAGAGGGAAGATGGG CGGGACCAACAGGTCCTGAAGCTGCGCGACCGGCGCGGGGGGCGCGGGGGCAGGCGCGAGGCGCCAGAGC TCGGTGGGACAGCTGGGAACACTCCAGACTACCTACACATCCTGGCCCAGGCTGGGACCAGTGCAGCCCC TCCTTCCTCTGTGCCCCCTCTTCCCAGAAACTCATCATGGAGAGTAAGGATGAAGTCAGTGACTCCGACA GCGGCATCATCCTGCAGTCTGGCCCGGACAGCCCTGTCTCTCCAATGAAAGAGCTGACCAATGCAGTACG CAAGCAGCAGAGGGCTCTGGAAGCGAGGCTGGAGGCCTGCCTGGAGGAGCTGCGCAGACTCTGCCTGAGG GAGGCGGAGCTGACTGGGACTTTGCCGGCAGAGTATCCCCTTAAGCCTGGTGAGAAGGCCCCCAAGGTCC GGCGCAGGATCGGAGCAGCGTACAAGCTGGATGAGTGGGCCCTCCACAGAGAGGACCCCCTGAGCAGCCT AGAGCGCCAGTTGGCTCTGCAGCTTCAGATCACCGAGGCGGCTCGAAGGCTGTGTGCAGAGGAGAACCTC AGCAGACAGGCACGCAGGCAGCGGAAGCACGCTGCGCTGCAGGAGGAGAAGAAGCTGCGGGACCTTCAGC GTTGCCTGGGTGATCGGCGGCGGAACAGTGAGCCCCCTCCTACCACTGTGCCCTCCCTGGGCAGAGAACT CAGTGCCTCTGACGACAGCTCCCTGTCTGATGGGCTGCTCCTGGAGGAAGAAGACTCCCAAGCGCCAAAA CCTCCCCCAGAGTCACCGGCACCACCTTCGCGGCCTCTCCCACCCCAGAGCCTTGAGGGCCTACAGCCAA CAGGACCTGAGTCTGGCGGCCAGGAACGGGCTCCCATCCAAAACAGTCCCTGGAAGGAGACCAGCCTGGA CCACCCCTATGAGAAGCCCAGGAAGTCTTCTGAGCTCAGTAGCGAGTCCAGCAGCCCGGCCACCACTCCT CAGGACCAGCCCAACCCTTCCAGCCTGTGGGTGCTGGATGCTGCCTCTTACCACGTGGTCCCCATCCGAA ATGTTCCCGGCCAGCGTCAGGGCCGCACCAGTGCCCCAGCCACCCCTGAGATGCAGGGCAGGAGGGGCCA GTCACAGTCTCTGAGGGTGGACTCCTTCCGAGCAGGCGCGGAGGGCCGAGGTCGCAGTGCCTTTCCCCGC CGCCGCCCCACTCATTACACAGTGACAGTGCCAGACTCCTGCTTCACACCCGGCAAGCCCCCACTGCCAC ACCCTGCCTGCCATTCCTGCTCTGAAGACAGTGGCTCTGATGTCTCCAGCATCTCCCATCCCACCTCACC TGGCAGCAGCAGTCCAGACATCTCCTTTCTGAGGCCCCTCTGTCTCCCTGAGCCACCTCGCCACCGTGGG GCCTGGGGCCCAGCCTGTGGCAGAGAGCTGGCCCCTCATTACTCTAAACTTCTGCTACCCGCTGGATACT TCCCAACGGGGCGCTATGTGATGGTGGCCGAGGGCCACCTGCCGCCGGGCGAGTGGGAGCTGTGTCGTGC AGCCGTGGGTGCTGCCTATGACGAGGAAGGTGCACCCCTGCGTTACCAGCGACTGGTACCCTCCCACAGC CGCATTGTGCGCACACCCTCACTAAAGGACAGTCCTGCGGGCCGGGGACTCAGCAAGGCAGCAGTCTCGG AGGAGCTCAAGTGGTGGCATGAACGCGCACGTCTCCGGAGCAGCCGCCCCCACTCGCTCGACCGCCAAGG GGCGTTCCGTGTTAGGAGTCTGCCTCCTGGGAGGGAGAGCTTCGGGCGGGCCTCAGGACCCAGGACACAG GTGCCCCCCGTGTACGTACTCCGGAGGTCGACGGACGGGGCCCCAGTGCAAGTCTTTGTGCCTGAGAACG GAGAGATTATCAGCCAAGTGTAACTCTTCTGAACGCCTTGCCTCGTGCGGGAAAAGACTTTTCTATTGGA GCTGGGGTCGAGGACCCCCTCACCCTTAAGTGCTTCTTTCATCTCCCCTCCCCTCATCGCAGGCCGATGA TGCAGAGCTGAGACCTTTCGGTTTTTTTACATGTGTTTCCCAGAAGTCCTGACCTGAGGATTTATATCTG TGATTTGTCTTATTCTTTAATAACATGATTATGCTTTATCCCCCAGAGTCTGGCCTTTTGGACTAATGTC GAGCTGGACTAATCGACAGCATTTGTCTCCTTATGTAGGACAAGTGGCATAGAGGGCCCCTTGAGACGTC CCATATGCTCTGGGCCCTCTTTCTGAGATACCTGTTGCTTCTTAAGGCTGGAGGCCGGATGTGGGACTCC CTCAACAGGGACTCTTCCCTTGTTTCCCAGATGCTGGGCAGAACTCTCCTCTCCCCTTCAGGGCACAGGC ATTTCTGTCATGGTGTTCTTCCCATACCCTACAGTAGTCCCAACCTCCACTTGCCATATTGTATGGCTTC TGAGCCCCTTCCCCAGTGCATAGATGCCCCCCCCCCAACCCAGTGTATTCTCTGGATCCCTAAGTGCCTT AGATCCTGAGCCACAGAGCTGAGAGACTTCTGAGTGCTAGTGCCTCCCCACACCTGAGCATCCTTCACAT TAATTAACTTATTGGTCTTACCTAACAGCTATCTCGTGTGGTTGCACCGTGGCGGTGCCCTCCGTTACAG GGCAACCTTGTGCCAAATACTTCAATAAAATCTGATTGGCCCAGCTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6402346 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6402346 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7341 same embryo
	EMAGE:7342 same embryo
	EMAGE:7343 same embryo
	EMAGE:7339 same embryo
	EMAGE:7338 same embryo
	EurExpress:euxassay_005271 same experiment
	MGI:4822769 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source