EMAGE mouse gene expression spatial database

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EMAGE:7419

Lypla1 lysophospholipase 1 ( MGI:1344588)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004439_01	euxassay_004439_02	euxassay_004439_03	euxassay_004439_04

euxassay_004439_05	euxassay_004439_06	euxassay_004439_07	euxassay_004439_08	euxassay_004439_09

euxassay_004439_10	euxassay_004439_11	euxassay_004439_12	euxassay_004439_13	euxassay_004439_14

euxassay_004439_15	euxassay_004439_16	euxassay_004439_17	euxassay_004439_18	euxassay_004439_19

euxassay_004439_20	euxassay_004439_21	euxassay_004439_22	euxassay_004439_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7419_wholemount_strong.wlz

7419_wholemount_moderate.wlz

7419_wholemount_weak.wlz

7419_wholemount_possible.wlz

7419_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7419_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7547
Entity Detected:	Lypla1, lysophospholipase 1 ( MGI:1344588)
Sequence:	sense strand is shown >T7547 GCACTGTCCGCCAGCCGGTGGATGTGCGGCAACAACATGTCCGCTCCGATGCCCGCCGTTGTGCCGGCCG CCCGGAAGGCCACCGCCGCGGTTATTTTCCTTCACGGATTGGGAGATACAGGGCATGGATGGGCAGAAGC CTTTGCAGGTATCAAAAGTCCCCACATCAAATACATCTGTCCACATGCCCCTGTGATGCCAGTCACATTA AATATGAATATGGCTATGCCTTCTTGGTTTGATATCGTTGGACTTTCACCAGATTCCCAGGAAGATGAAT CTGGAATTAAACAGGCAGCAGAAACCGTAAAAGCCTTGATAGATCAAGAAGTGAAGAATGGCATTCCTTC TAACAGGATTATTTTGGGAGGATTTTCTCAGGGAGGCGCCTTGTCTTTATACACTGCTCTCACCACACAG CAGAAACTGGCTGGTGTCACTGCACTCAGTTGCTGGCTTCCACTTCGGGCTTCGTTTTCACAGGGGCCGA TCAACAGTGCTAATCGAGATATTTCCGTTCTCCAGTGCCATGGAGATTGTGACCCTTTAGTTCCCCTAAT GTTTGGTTCTCTTACTGTTGAAAGACTAAAAGCATTGATAAATCCAGCCAATGTAACCTTCAAAATCTAT GAAGGCATGATGCACAGCTCATGTCAGCAGGAAATGATGGATGTCAAGCACTTCATTGATAAGCTCCTAC CTCCAATTGATTGACATCACTAAGAGGCCTTGAGTAGAAGTTCACCAGCATCACAGTAGTAGAGTATAAA CCTCTTCCCATGACTGACCATAGAACTTCTAATGTGTGCAGTGTTAAAATGTTTTGCAAATACATACCAA TGATACAGACTAAATGATGTTACCCTCATGGGAAATATATGATCCTTTTAAGTGTCTATGCATATATTTG TATTATACAATTCAGAATATACTAAGTATTACAGTAGGTAAGTGAAGCAACTAGATTCTTTGTCTCAAAT TCAGCAAAATAAGATAACAAACAGTTTTATTACATCATTTGAAAATTACTAGTATGTTCTGTGAAGATGT GTTCAGGTAAGATGTAAGTGACTTCTTAGTTCTGTGAGTTAGACTATAGTTTTACTCCACAATTGTTCAG TCTCCATGCAGTGTCTGTATTTTTCTGTTTGTATTCACATAGATGATACTATAATAGCTAAGAATCAGAT GGTATTACATTATCCCTAATAATGGGGATAATGCTTTTGAGTGTCAAAAAGTAATACTGCATTCTTCAAT TAATGGCCCTTCTATTGGGAGACCAGGCTTTTTTTCCCTTCTATGTAATTTTTCTCAAGAAAAAACAAGG TTCTTTTTTGATTTTCCGTAGAAAACTAATATTGCCTCTCTGTCATATATTTTATTTTGACATTTTGAGA TACAGACATAGCCGGGCGTGGTGGTGCATGCCTTTAATCCCAGCACTCGGGAGGCAGAGGCAGGCGGATC TCTGTCAATAGCCTGGTCCACAGAGTGAGTTCCAGGACAGCCAGGGCTACACAGAGAAACCCTGTCTCGG AAACAAACAAACAAACAAACCGAAACACAGACATACAGTATTTCCAACCTAGGTAATATGAAAAGAAATC AAACATTACTTAAGTTTATATATTTCAAATAACTGCACCTTAGTCCAACTTTAGACTGTAGAGTTATAGA CTCTCCAGTCCTGGTAATAAATCTGTGGTTTTATTTTTCTGTAAAACACTTAAAGGGGAAGTAATATCTT GGCATATTTAGAAATACTTGTGACTGGCTTCAAAATATAATTTCTTAAATATCTTATAGCCGATTTTAAG TTTACATTTTCTGTACATGTTATGCTTAAAACCAAATACTGTTCAAGATGAGCAATCAGACTTATGTTTT ATTTTTATCTGGGTAACATATTATTTTGAAATTTTCATCTTAAGTAAAGGAGCTTTTGATAATCAGTCTC CTGTACAAATATTAAAATAATAATTTGATTTTAAATCATCAGCATGTTCAAAATACTGGTGAAAATTATT TAATATAAGGACTGGTTAGAACATTATATATATATCCCATATTTATAACTGTTACCACACAGTTGAATAT GTTGCAAAAATTATGGTTATTTCTGAACGGTTGCTTTTCTTGCTGTTTTCCAACCAACCAAGAAGCACCT TTATAAACAGGTGCTAATTGATATGAAGATCATTTTGGTATGTCCTAGATAGAATGAAGCCAAATGTCTG GATTTAAAACAATTTCAAACAATCATTTACTATTTTGCTTTACTTTAAAGAACATAAATTTACTTCAGTT ATTCTATCTGTAAAGATTTATTTGGTATAAGAAATATTCCATTGACTGTGTGCTGTAATGTGGGAAAATG TCATGTTTTTCACAGTTTCTATCAATGTGAAATAAAATTTAATTTTGGCTTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4240573 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240573 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7422 same embryo
	EMAGE:7420 same embryo
	EMAGE:7423 same embryo
	EMAGE:7418 same embryo
	EMAGE:7421 same embryo
	EurExpress:euxassay_004439 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source