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EMAGE:7420

Hist2h3c2 histone cluster 2, H3c2 ( MGI:2448357)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420
"Pseudo-wholemount" of euxassay_004437. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004437_01 euxassay_004437_02 euxassay_004437_03 euxassay_004437_04
EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420
euxassay_004437_05 euxassay_004437_06 euxassay_004437_07 euxassay_004437_08 euxassay_004437_09
EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420
euxassay_004437_10 euxassay_004437_11 euxassay_004437_12 euxassay_004437_13 euxassay_004437_14
EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420
euxassay_004437_15 euxassay_004437_16 euxassay_004437_17 euxassay_004437_18 euxassay_004437_19
EMAGE:7420 EMAGE:7420 EMAGE:7420 EMAGE:7420
euxassay_004437_20 euxassay_004437_21 euxassay_004437_22 euxassay_004437_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7420Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7420_wholemount_strong.wlz
7420_wholemount_moderate.wlz
7420_wholemount_weak.wlz
7420_wholemount_possible.wlz
7420_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7420_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7550
Entity Detected:Hist2h3c2, histone cluster 2, H3c2 ( MGI:2448357)
Sequence:sense strand is shown

>T7550
GTGGCGGGCCCGAGCCAATGGGCGAGGTCGGGGCCGGTGACGCCACGGCCAATGGCGCGGCAGCGCGGGA
GTTTCAAGTCGCTGTCTCCGCCCCGCCGCGGGGAAGACTGCGCCTATAAAGGCGGCCGGCTCGGGCCGGT
ATCAGGTCCCCGAGTGCTCCCCGTTTGGGTGTCTTCCGTCTTCGCCATGGCCCGTACGAAGCAGACTGCC
CGCAAGTCCACCGGCGGCAAGGCCCCGCGCAAGCAGCTGGCCACCAAGGCCGCCCGCAAGAGCGCCCCGG
CCACGGGCGGCGTGAAGAAGCCGCACCGCTACCGGCCCGGCACCGTGGCGCTGCGGGAGATCCGGCGCTA
CCAGAAGTCGACCGAGCTGCTGATCCGCAAGCTGCCGTTCCAGCGCCTGGTGCGCGAGATCGCGCAGGAC
TTCAAGACGGACCTGCGCTTCCAGAGCTCGGCCGTCATGGCGCTGCAGGAGGCGAGCGAGGCCTATCTGG
TGGGGCTGTTCGAGGACACCAACCTGTGCGCCATCCACGCCAAACGCGTCACCATCATGCCCAAGGACAT
CCAGTTGGCCCGCCGCATCCGTGGGGAGCGCGCTTAAGCGCCCTGTCTCCCATCCATTCCCCACAAAGGC
TCTTTTCAGAGCCACCACAGCTTCCCGGAAAGAGCTGTTTACTTCGCTTAGAGCTTTGTCCGCACATTAT
CCCGCTCCATAGCTCTAGGTTGGTATCTGACTTATACCTGTTATGTCTACATAATGGTTGCTTTCAAGGA
AGGTTTGAATGCCCTTAGTGTAAGTTGTTTAACGTTAATTGCGGTCTTATTCAGACTCCTAGCTTGTTAA
CCCGAAGTAGGTTCACCACCTCAGTCTAAGGGGAGGGGATAATCTTACGGAGGGTTGGGGCTTGGTGGAT
ACTATGTAATGTTCCTACCCAGCTGGAGCAGAGAAGGCTTTGTAAGTAGCTGAAGGCTGAGATCTAGCTT
ACAGCTCATAATTACATCACCAGACTTCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAA
Notes:The probe template was PCR amplified from IMAGE:4240748 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4240748 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7422 same embryo
 EMAGE:7423 same embryo
 EMAGE:7419 same embryo
 EMAGE:7418 same embryo
 EMAGE:7421 same embryo
 EurExpress:euxassay_004437 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS